Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,239	C→A	11.7%	L178M (CTG→ATG) ‡	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,239	0	C	A	11.7%	240.3 / 10.2	76	L178M (CTG→ATG) ‡	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base C (38/29);  new base A (5/4);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGG  >  minE/132169‑132309                                                                       |                                                                       gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                                                                        >  1:3282126/1‑71 (MQ=255)   tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                                      >  1:3699296/1‑71 (MQ=255)   tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                                      >  1:1378704/1‑71 (MQ=255)        gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                  <  1:2961241/70‑1 (MQ=255)           atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                              >  1:312576/1‑71 (MQ=255)           atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                              >  1:524861/1‑71 (MQ=255)           atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                  <  1:232113/67‑1 (MQ=255)            tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                              >  1:3279009/1‑70 (MQ=255)            tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                              >  1:1589625/1‑70 (MQ=255)              aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                           <  1:3378901/71‑1 (MQ=255)              aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                           <  1:3916233/71‑1 (MQ=255)              aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                           <  1:1866093/71‑1 (MQ=255)               ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                                                 >  1:501805/1‑64 (MQ=255)               ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                           >  1:369444/1‑70 (MQ=255)               ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                           <  1:895428/70‑1 (MQ=255)               ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                                          >  1:3018776/1‑71 (MQ=255)                cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                         >  1:3459369/1‑71 (MQ=255)                cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                         >  1:1094608/1‑71 (MQ=255)                aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                         >  1:2295944/2‑71 (MQ=255)                 aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                         >  1:1704122/1‑70 (MQ=255)                  tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                            >  1:413424/1‑66 (MQ=255)                  tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                              <  1:3129806/64‑1 (MQ=255)                  tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                                        >  1:2795204/1‑70 (MQ=255)                  tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       <  1:822055/71‑1 (MQ=255)                   cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                                       <  1:353302/67‑1 (MQ=255)                   cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                            >  1:1445872/1‑65 (MQ=255)                    acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       >  1:938210/1‑69 (MQ=255)                     cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                      <  1:1085283/69‑1 (MQ=255)                      acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                                                                       <  1:2242153/51‑1 (MQ=255)                      acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                   >  1:1215807/1‑71 (MQ=255)                      acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                   >  1:3892751/1‑71 (MQ=255)                         ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                                <  1:1739920/71‑1 (MQ=255)                            tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                   >  1:3322513/1‑65 (MQ=255)                             gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                  <  1:368264/49‑1 (MQ=255)                             gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                  <  1:2582517/49‑1 (MQ=255)                               cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       <  1:3826494/58‑1 (MQ=255)                               cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       <  1:2036055/58‑1 (MQ=255)                                gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                           <  1:2685332/66‑1 (MQ=255)                                  aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                               <  1:3338305/47‑1 (MQ=255)                                  aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                       >  1:74025/1‑71 (MQ=255)                                   aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                                 <  1:3299476/44‑1 (MQ=255)                                   aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                               >  1:2171812/1‑46 (MQ=255)                                    tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                     >  1:477006/1‑71 (MQ=255)                                    tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                     <  1:3405915/71‑1 (MQ=255)                                     cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                    >  1:643085/1‑71 (MQ=255)                                       tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                >  1:2239041/1‑41 (MQ=255)                                        gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                          >  1:1559300/1‑62 (MQ=255)                                        gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                                 >  1:664640/1‑71 (MQ=255)                                         cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                                <  1:1122052/71‑1 (MQ=255)                                          aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                               <  1:1854168/71‑1 (MQ=255)                                             aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                            >  1:1491341/1‑71 (MQ=255)                                              tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                            >  1:507433/1‑70 (MQ=255)                                              tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                           <  1:589586/71‑1 (MQ=255)                                              tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                           <  1:3760758/71‑1 (MQ=255)                                                 gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                        <  1:1494823/71‑1 (MQ=255)                                                  cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                       >  1:1326631/1‑71 (MQ=255)                                                   ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                      >  1:3369448/1‑71 (MQ=255)                                                   ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                      >  1:2282189/1‑71 (MQ=255)                                                     aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                   <  1:2972567/40‑1 (MQ=255)                                                     aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                    >  1:290260/1‑71 (MQ=255)                                                       tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                       >  1:3139451/1‑50 (MQ=255)                                                       tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa                  >  1:3660091/1‑71 (MQ=255)                                                       tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa                  >  1:2096639/1‑71 (MQ=255)                                                         cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                     >  1:3594428/1‑50 (MQ=255)                                                         cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                 <  1:1619129/70‑1 (MQ=255)                                                         cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                 <  1:1040486/70‑1 (MQ=255)                                                         cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACg                >  1:2472892/1‑71 (MQ=255)                                                           aTTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                <  1:2496046/53‑1 (MQ=255)                                                            ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                                 <  1:66698/51‑1 (MQ=255)                                                            ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt              >  1:2838467/1‑70 (MQ=255)                                                            ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt              >  1:960573/1‑70 (MQ=255)                                                             tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                            <  1:3322401/39‑1 (MQ=255)                                                              ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa            <  1:1305193/70‑1 (MQ=255)                                                              ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa            <  1:721548/70‑1 (MQ=255)                                                              ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt           >  1:110300/1‑71 (MQ=255)                                                               tcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                          >  1:545898/1‑39 (MQ=255)                                                                 gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                               >  1:1969613/1‑48 (MQ=255)                                                                 gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcga        <  1:2385048/71‑1 (MQ=255)                                                                   actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacg      <  1:3109764/71‑1 (MQ=255)                                                                     tactGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                     >  1:3852576/1‑38 (MQ=255)                                                                       ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa    <  1:895279/69‑1 (MQ=255)                                                                       ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAgg  >  1:3032326/1‑71 (MQ=255)                                                                       ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg   <  1:3807875/70‑1 (MQ=255)                                                                       |                                                                       GCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGG  >  minE/132169‑132309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑