| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 356,222 | G→C | 6.2% | H199D (CAC→GAC) | folD ← | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 356,222 | 0 | G | C | 6.2% | 300.9 / 7.0 | 97 | H199D (CAC→GAC) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (46/45); new base C (4/2); total (50/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
ATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCC > minE/356155‑356286 | aTCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGtgat < 1:647548/70‑1 (MQ=255)aTCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGtgat > 1:750706/1‑70 (MQ=255)aTCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGtgat > 1:1230065/1‑70 (MQ=255) tCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGtgatga > 1:2841245/1‑71 (MQ=255) ccAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGAc > 1:2418920/1‑71 (MQ=255) ccAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGAc > 1:2186257/1‑71 (MQ=255) cAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGAc < 1:2221831/70‑1 (MQ=255) cAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACg > 1:1161135/1‑71 (MQ=255) cAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACg < 1:3303227/71‑1 (MQ=255) aGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACg > 1:1397475/1‑70 (MQ=255) tCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAg < 1:1766409/71‑1 (MQ=255) cACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAg < 1:938132/70‑1 (MQ=255) aCCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAt < 1:1173427/71‑1 (MQ=255) aCCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAt > 1:1614411/1‑71 (MQ=255) ccGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTGCGTCATCACGTAGaaa < 1:871102/71‑3 (MQ=38) ccGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTGCGTCATCACGTAGaaa < 1:3097243/71‑3 (MQ=38) gggAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGc < 1:3658331/64‑1 (MQ=255) gggAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt > 1:2089001/1‑71 (MQ=255) gggAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt < 1:1660627/71‑1 (MQ=255) ggAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAttttt < 1:1541796/71‑1 (MQ=255) ggAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt > 1:3431266/1‑70 (MQ=255) ggAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt > 1:1149837/1‑70 (MQ=255) aTAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAg < 1:371620/70‑1 (MQ=255) aTAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGt < 1:2377896/71‑1 (MQ=255) aaGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTa > 1:479060/1‑66 (MQ=255) aaGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTg > 1:3521415/1‑69 (MQ=255) aaGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGa < 1:70644/70‑1 (MQ=255) aaGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGa < 1:2973903/70‑1 (MQ=255) aGCCTGTCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAg < 1:2967062/71‑1 (MQ=255) aGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAg < 1:1609524/71‑1 (MQ=255) aGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAg < 1:147513/71‑1 (MQ=255) cTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGc > 1:1214753/1‑69 (MQ=255) gCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt > 1:2416302/1‑57 (MQ=255) gCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGt < 1:3223746/69‑1 (MQ=255) gCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGt > 1:1220474/1‑69 (MQ=255) gCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGa < 1:2655944/71‑1 (MQ=255) cTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTGCGTCATCACGTAGaa > 1:3724851/1‑52 (MQ=25) tGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGt > 1:3341468/1‑70 (MQ=255) gCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTc > 1:379525/1‑70 (MQ=255) ccAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGt < 1:904135/64‑1 (MQ=255) ccAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCa < 1:219120/70‑1 (MQ=255) cAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACt > 1:1783385/1‑71 (MQ=255) aaCGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTg < 1:2891818/71‑1 (MQ=255) aCGGCAACGATCAATAGATCGGCATTTTCTGCGTCATCACGTAGaa > 1:1398935/1‑45 (MQ=21) aCGGCAACGATCAATAGATCGGCATTTTCTGCGTCATCACGTAGaa > 1:3674418/1‑45 (MQ=21) aCGGCAACGATCAATAGATCGGCATTTTCTGCGTCATCACGTAGaa > 1:140988/1‑45 (MQ=21) aCGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTa > 1:333005/1‑51 (MQ=255) aCGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTa > 1:1558075/1‑51 (MQ=255) aCGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCa > 1:1769264/1‑67 (MQ=255) aCGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTg > 1:1197997/1‑70 (MQ=255) aCGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTg > 1:3836879/1‑70 (MQ=255) aCGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGt < 1:3110618/71‑1 (MQ=255) cGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTg > 1:771315/1‑69 (MQ=255) cGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTg > 1:530555/1‑69 (MQ=255) cGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTg > 1:229627/1‑69 (MQ=255) ggCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAg < 1:189688/71‑1 (MQ=255) ggCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAg < 1:501356/71‑1 (MQ=255) cAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt < 1:2545156/45‑1 (MQ=255) cAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt < 1:1317519/45‑1 (MQ=255) cAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAgtg < 1:1634308/71‑1 (MQ=255) aaCGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAgtgg > 1:3061742/1‑71 (MQ=255) cGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTg > 1:2888654/1‑47 (MQ=255) cGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTg > 1:24567/1‑47 (MQ=255) cGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTg > 1:2291782/1‑47 (MQ=255) cGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTg > 1:2097613/1‑47 (MQ=255) cGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAgtggtg > 1:301287/1‑71 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt < 1:3586129/41‑1 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt < 1:2470064/41‑1 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGAtttt < 1:2508337/41‑1 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTa > 1:2601648/1‑43 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTa > 1:1961045/1‑43 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAg > 1:3136976/1‑49 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAg < 1:3511481/65‑1 (MQ=255) gATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGc < 1:1283179/71‑1 (MQ=255) tCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTa > 1:24554/1‑41 (MQ=255) tCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCaa > 1:891079/1‑71 (MQ=255) aaTAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAAcc < 1:3683357/71‑1 (MQ=255) aaTAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAAc < 1:2640658/70‑1 (MQ=255) gATCGGCATTTTCTACGTGATGACCCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCt > 1:3896844/1‑68 (MQ=255) aTCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCa > 1:1118347/1‑63 (MQ=255) aTCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCa > 1:1068672/1‑63 (MQ=255) cGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcag > 1:502660/1‑70 (MQ=255) ggCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagca < 1:2399872/71‑1 (MQ=255) ggCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagca < 1:1812996/71‑1 (MQ=255) ggCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagca < 1:271204/71‑1 (MQ=255) ggCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagca < 1:1421071/71‑1 (MQ=255) ggCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagca < 1:1300857/71‑1 (MQ=255) gCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagcag > 1:3922411/1‑71 (MQ=255) gCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagcag < 1:1488086/71‑1 (MQ=255) gCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagcag < 1:1267342/71‑1 (MQ=255) cATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagcagc < 1:1685698/71‑1 (MQ=255) aTTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagcagca < 1:1672339/71‑1 (MQ=255) aTTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCcagcag > 1:1810453/1‑69 (MQ=255) ttttCTACGTGATGACGCAGATTTTTAGTGAAGCgg > 1:3148232/1‑36 (MQ=255) ttttCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAAc < 1:1427141/58‑1 (MQ=255) ttCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCa > 1:1340208/1‑41 (MQ=255) tCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAgtggt > 1:1441213/1‑50 (MQ=255) tCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGtt < 1:722935/70‑1 (MQ=255) tACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAgtggt > 1:445424/1‑48 (MQ=255) gTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTcc < 1:515493/67‑1 (MQ=255) | ATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCC > minE/356155‑356286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |