| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 359,423 | T→A | 7.2% | W218R (TGG→AGG) | sfmD → | predicted outer membrane export usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 359,423 | 0 | T | A | 7.2% | 234.4 / 8.7 | 83 | W218R (TGG→AGG) | sfmD | predicted outer membrane export usher protein |
| Reads supporting (aligned to +/- strand): ref base T (39/38); new base A (4/2); total (43/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
GATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGC > minE/359354‑359491 | gATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTg > 1:3903480/1‑71 (MQ=255) tAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGa < 1:1345127/71‑1 (MQ=255) cTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACt < 1:2845743/71‑1 (MQ=255) cTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACt < 1:3310381/71‑1 (MQ=255) tttttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTAtt > 1:943216/1‑71 (MQ=255) tttttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTAtt > 1:2800852/1‑71 (MQ=255) ttttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTAttt > 1:3097954/1‑71 (MQ=255) tttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCt > 1:2677009/1‑60 (MQ=255) tttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCt > 1:2594310/1‑60 (MQ=255) ttttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTc > 1:3697154/1‑71 (MQ=255) tttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTc < 1:1952200/70‑1 (MQ=255) tttAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTc < 1:2370498/70‑1 (MQ=255) tttAGTGAGCTAAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTc < 1:1201137/70‑1 (MQ=255) aGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTg > 1:583824/1‑56 (MQ=255) aGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTa < 1:2406506/62‑1 (MQ=255) aGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcgcg < 1:3149208/70‑1 (MQ=255) gTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAAc < 1:1753766/59‑1 (MQ=255) gTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGa < 1:699160/71‑1 (MQ=255) tGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGaa < 1:306744/71‑1 (MQ=255) aGCTCAGCGTGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAAt < 1:2675801/71‑1 (MQ=255) aGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTc < 1:3205524/63‑1 (MQ=255) aGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGaaa < 1:3555589/70‑1 (MQ=255) aGCTAAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTg < 1:2537574/52‑1 (MQ=255) cTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATgg < 1:2468665/71‑1 (MQ=255) tCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTgg < 1:3075681/50‑1 (MQ=255) tCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTgg < 1:3045602/50‑1 (MQ=255) tCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGa > 1:2853392/1‑71 (MQ=255) cAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGa > 1:2623568/1‑70 (MQ=255) aGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTa < 1:1799914/53‑1 (MQ=255) aGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGata > 1:303934/1‑71 (MQ=255) aGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGat < 1:2332296/70‑1 (MQ=255) cGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGatat > 1:518109/1‑70 (MQ=255) aaTATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACt < 1:1604878/43‑1 (MQ=255) aaTATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAg > 1:960523/1‑70 (MQ=255) atatTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGaa > 1:3390950/1‑71 (MQ=255) ttGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAg > 1:3087913/1‑71 (MQ=255) ttGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAg > 1:1798006/1‑71 (MQ=255) ggCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcgc > 1:2818704/1‑45 (MQ=255) gCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcg < 1:2869156/43‑1 (MQ=255) gCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTg > 1:2443327/1‑70 (MQ=255) cccGTGGCGTTTACGCAACAATGGTTCCTGGAACCATTTTCGCGGAAATGGATATCATTCAGAACAGTgg > 1:3140493/1‑70 (MQ=255) cccGTGGCGTTTACGCAAAAATAGTTCCAGGAACCAttg < 1:2424985/39‑2 (MQ=11) cccGTGGCGTTTACGCAAAAATAGTTCCAGGAACCAttg < 1:697921/39‑2 (MQ=11) ccGTGGCGTTTACGCAACAATGGTTCCTGGAACTAtt < 1:3366346/37‑1 (MQ=255) ccGTGGCGTTTACGCAACAATGGTTCCTGGAACTAtt < 1:1482775/37‑1 (MQ=255) ccGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcgcg > 1:2234246/1‑43 (MQ=255) ccGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTcgcg > 1:2174738/1‑43 (MQ=255) cGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATgg > 1:3887220/1‑49 (MQ=255) cGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaa > 1:675094/1‑70 (MQ=255) cGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaa > 1:2917323/1‑70 (MQ=255) gTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCAtt > 1:951174/1‑56 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGatat < 1:2949179/49‑1 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAataata < 1:1103300/71‑1 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAataata < 1:587262/71‑1 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAataata < 1:2780740/71‑1 (MQ=255) gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAataata > 1:1568784/1‑71 (MQ=255) gTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATTGATATCATTCAGAACAGTGGAATAATAtt < 1:2253011/71‑1 (MQ=255) ttACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTgg > 1:268936/1‑71 (MQ=255) ttACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTgg > 1:80757/1‑71 (MQ=255) ttACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTg > 1:3409074/1‑70 (MQ=255) tACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATAtt > 1:228967/1‑68 (MQ=255) tACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTgg > 1:1499663/1‑70 (MQ=255) tACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTgg > 1:1988102/1‑70 (MQ=255) tACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTgg > 1:2350868/1‑70 (MQ=255) tACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTg > 1:1049441/1‑69 (MQ=255) cGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCa > 1:727291/1‑70 (MQ=255) cGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCAc < 1:3557522/71‑1 (MQ=255) gCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAatat < 1:3729971/64‑1 (MQ=255) caacaaTGGTTCCTGGAACTATTTTCGCGGAAATTGATATCATTCAGAACAGTGGAATAatat < 1:282161/63‑1 (MQ=255) caacaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCt < 1:2059998/71‑1 (MQ=255) aacaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaataat < 1:2185194/60‑1 (MQ=255) acaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaat < 1:1506794/56‑1 (MQ=255) caaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaataat > 1:110996/1‑58 (MQ=255) caaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTggg < 1:2045159/71‑1 (MQ=255) caaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTgg > 1:2165305/1‑70 (MQ=255) aaTAGTTCCAGGAACCATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCt > 1:1388056/1‑67 (MQ=38) aaTAGTTCCAGGAACCATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCt > 1:3434011/1‑67 (MQ=38) aaTAGTTCCAGGAACCATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCt > 1:3779825/1‑67 (MQ=38) aaTAGTTCCAGGAACCATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCt > 1:1832313/1‑67 (MQ=38) aTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGaataa < 1:2849541/55‑1 (MQ=255) aTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTa < 1:483774/71‑1 (MQ=255) tCCTGTAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATAtt > 1:3177618/1‑54 (MQ=255) tCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATAtt > 1:1883310/1‑54 (MQ=255) ccTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAgcgc > 1:860939/1‑71 (MQ=255) cTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATAtt > 1:2233101/1‑52 (MQ=255) tGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCt < 1:1441385/58‑1 (MQ=255) | GATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGC > minE/359354‑359491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |