| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,069 | T→C | 10.9% | F93F (TTT→TTC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,069 | 0 | T | C | 10.9% | 169.4 / 10.8 | 64 | F93F (TTT→TTC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (25/32); new base C (2/5); total (27/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01 | |||||||||||
CAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGG > minE/384999‑385137 | cAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtttt > 1:2700143/1‑71 (MQ=255) aGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTg > 1:1512428/1‑71 (MQ=255) gCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGc < 1:3435647/71‑1 (MQ=255) gCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGc < 1:2525464/71‑1 (MQ=255) cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTTCTGTTCGGTCACCATTTTGCGGCCa < 1:1465084/70‑1 (MQ=255) cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCg > 1:2037583/1‑66 (MQ=255) cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc < 1:1564063/68‑1 (MQ=255) cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc < 1:543015/68‑1 (MQ=255) gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:3732035/71‑1 (MQ=255) gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:41723/71‑1 (MQ=255) gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:1943463/71‑1 (MQ=255) ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTg < 1:3269649/71‑1 (MQ=255) ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTg > 1:2884745/1‑71 (MQ=255) aTAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc < 1:3678626/71‑1 (MQ=255) tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCg > 1:3654825/1‑61 (MQ=255) aaCGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc > 1:1783773/1‑62 (MQ=255) aaCGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc > 1:3851878/1‑62 (MQ=255) cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg > 1:2086705/1‑71 (MQ=255) gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGc < 1:3376753/71‑1 (MQ=255) aggggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCgg < 1:3757942/65‑1 (MQ=255) cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:1576451/63‑1 (MQ=255) cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCa < 1:3265336/70‑1 (MQ=255) gCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCgg > 1:2801818/1‑53 (MQ=255) gCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCg > 1:3700973/1‑52 (MQ=255) gCTGGACTATGTGACGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCgg > 1:2326731/1‑53 (MQ=255) cttgACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGTCCATTGCCGGAGCAGGTcc < 1:1972803/68‑1 (MQ=255) gACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGc < 1:3340742/70‑1 (MQ=255) aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg > 1:3081190/1‑71 (MQ=255) aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg > 1:1799774/1‑71 (MQ=255) cTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtgg < 1:329394/71‑1 (MQ=255) cTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtgg < 1:784927/71‑1 (MQ=255) tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt > 1:3048428/1‑71 (MQ=255) tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtgg < 1:3058147/71‑1 (MQ=255) tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt < 1:3521552/69‑1 (MQ=255) gtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc < 1:2067321/51‑1 (MQ=255) gtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg < 1:435790/63‑1 (MQ=255) tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTgggg < 1:3075079/71‑1 (MQ=255) tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTT‑CGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGc < 1:525590/71‑1 (MQ=255) gCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAt > 1:128646/1‑45 (MQ=255) ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:700735/47‑1 (MQ=255) cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTg < 1:3448280/45‑1 (MQ=255) aCGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg > 1:666472/1‑49 (MQ=255) aCGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg > 1:2809642/1‑70 (MQ=255) ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc > 1:1463868/1‑71 (MQ=255) cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCg < 1:2910785/41‑1 (MQ=255) cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg < 1:29184/71‑1 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCt > 1:2987707/1‑53 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCt > 1:2453953/1‑53 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc > 1:1271111/1‑71 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg > 1:3370545/1‑70 (MQ=255) acgtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg > 1:2778902/3‑71 (MQ=255) aaGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggcggc < 1:952885/70‑1 (MQ=255) aGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc < 1:1889097/62‑1 (MQ=255) ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGAt < 1:2429710/70‑1 (MQ=255) cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCaaa > 1:1152441/1‑70 (MQ=255) ttCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATggg > 1:491594/1‑71 (MQ=255) ttCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATg < 1:2390870/69‑1 (MQ=255) gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTAc < 1:788121/71‑1 (MQ=255) caccacTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCtcg > 1:553342/1‑50 (MQ=255) aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg > 1:633270/1‑71 (MQ=255) aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTg < 1:3351358/71‑1 (MQ=255) ccATTTTGCGGCCATTGCCCGAGCAGGACCGCTGGTGGGGCCgct > 1:3282226/1‑43 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTTCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc < 1:3578533/71‑1 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc < 1:2743310/71‑1 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATAGGCTACCTGc < 1:1676088/71‑1 (MQ=255) tttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg > 1:2037630/1‑71 (MQ=255) ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg < 1:2008806/69‑1 (MQ=255) ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGAGGCGCAAATGGGCTACCTGCCggg < 1:938978/70‑1 (MQ=255) | CAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGG > minE/384999‑385137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |