| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,075 | C→T | 12.3% | A95A (GCC→GCT) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,075 | 0 | C | T | 12.3% | 149.7 / 10.7 | 57 | A95A (GCC→GCT) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base C (18/32); new base T (2/5); total (20/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
CGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCT > minE/385007‑385145 | cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTTCTGTTCGGTCACCATTTTGCGGCCa < 1:1465084/70‑1 (MQ=255) gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:41723/71‑1 (MQ=255) gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:1943463/71‑1 (MQ=255) gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:3732035/71‑1 (MQ=255) ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTg < 1:3269649/71‑1 (MQ=255) ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTg > 1:2884745/1‑71 (MQ=255) aTAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc < 1:3678626/71‑1 (MQ=255) cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg > 1:2086705/1‑71 (MQ=255) gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGc < 1:3376753/71‑1 (MQ=255) aggggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCgg < 1:3757942/65‑1 (MQ=255) cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:1576451/63‑1 (MQ=255) cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCa < 1:3265336/70‑1 (MQ=255) cttgACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGTCCATTGCCGGAGCAGGTcc < 1:1972803/68‑1 (MQ=255) gACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGc < 1:3340742/70‑1 (MQ=255) aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg > 1:1799774/1‑71 (MQ=255) aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg > 1:3081190/1‑71 (MQ=255) cTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtgg < 1:329394/71‑1 (MQ=255) cTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtgg < 1:784927/71‑1 (MQ=255) tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt > 1:3048428/1‑71 (MQ=255) tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtgg < 1:3058147/71‑1 (MQ=255) tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt < 1:3521552/69‑1 (MQ=255) gtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc < 1:2067321/51‑1 (MQ=255) gtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg < 1:435790/63‑1 (MQ=255) tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTgggg < 1:3075079/71‑1 (MQ=255) tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTT‑CGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGc < 1:525590/71‑1 (MQ=255) gCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAt > 1:128646/1‑45 (MQ=255) ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:700735/47‑1 (MQ=255) cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTg < 1:3448280/45‑1 (MQ=255) aCGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg > 1:666472/1‑49 (MQ=255) aCGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg > 1:2809642/1‑70 (MQ=255) ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc > 1:1463868/1‑71 (MQ=255) cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCg < 1:2910785/41‑1 (MQ=255) cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg < 1:29184/71‑1 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCt > 1:2987707/1‑53 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCt > 1:2453953/1‑53 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc > 1:1271111/1‑71 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg > 1:3370545/1‑70 (MQ=255) acgtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg > 1:2778902/3‑71 (MQ=255) aaGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggcggc < 1:952885/70‑1 (MQ=255) aGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc < 1:1889097/62‑1 (MQ=255) ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGAt < 1:2429710/70‑1 (MQ=255) cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCaaa > 1:1152441/1‑70 (MQ=255) ttCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATggg > 1:491594/1‑71 (MQ=255) ttCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATg < 1:2390870/69‑1 (MQ=255) gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTAc < 1:788121/71‑1 (MQ=255) caccacTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCtcg > 1:553342/1‑50 (MQ=255) aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg > 1:633270/1‑71 (MQ=255) aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTg < 1:3351358/71‑1 (MQ=255) ccATTTTGCGGCCATTGCCCGAGCAGGACCGCTGGTGGGGCCgct > 1:3282226/1‑43 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTTCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc < 1:3578533/71‑1 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc < 1:2743310/71‑1 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATAGGCTACCTGc < 1:1676088/71‑1 (MQ=255) tttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg > 1:2037630/1‑71 (MQ=255) ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg < 1:2008806/69‑1 (MQ=255) ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGAGGCGCAAATGGGCTACCTGCCggg < 1:938978/70‑1 (MQ=255) cGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatg < 1:3329337/71‑1 (MQ=255) gCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat < 1:2355728/71‑1 (MQ=255) ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGcc < 1:1011411/61‑1 (MQ=255) cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATTGGCTACCTGCCGGGGATGATCt < 1:3008884/71‑1 (MQ=255) cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt < 1:2075286/71‑1 (MQ=255) cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt > 1:1832261/1‑71 (MQ=255) cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt < 1:3106573/71‑1 (MQ=255) | CGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCT > minE/385007‑385145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |