Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,241	G→T	11.0%	L178L (CTG→CTT) ‡	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,241	0	G	T	11.0%	253.8 / 10.3	81	L178L (CTG→CTT) ‡	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base G (39/33);  new base T (5/4);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGG  >  minE/132171‑132310                                                                       |                                                                      tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                                       >  1:3699296/1‑71 (MQ=255) tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                                       >  1:1378704/1‑71 (MQ=255)      gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                   <  1:2961241/70‑1 (MQ=255)         atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                               >  1:312576/1‑71 (MQ=255)         atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                               >  1:524861/1‑71 (MQ=255)         atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                   <  1:232113/67‑1 (MQ=255)          tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                               >  1:3279009/1‑70 (MQ=255)          tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                               >  1:1589625/1‑70 (MQ=255)            aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                            <  1:3378901/71‑1 (MQ=255)            aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                            <  1:3916233/71‑1 (MQ=255)            aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                            <  1:1866093/71‑1 (MQ=255)             ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                                                  >  1:501805/1‑64 (MQ=255)             ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                            <  1:895428/70‑1 (MQ=255)             ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                            >  1:369444/1‑70 (MQ=255)             ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                                           >  1:3018776/1‑71 (MQ=255)              cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                          >  1:3459369/1‑71 (MQ=255)              cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                          >  1:1094608/1‑71 (MQ=255)              aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                          >  1:2295944/2‑71 (MQ=255)               aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                          >  1:1704122/1‑70 (MQ=255)                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                             >  1:413424/1‑66 (MQ=255)                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                               <  1:3129806/64‑1 (MQ=255)                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                                         >  1:2795204/1‑70 (MQ=255)                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                        <  1:822055/71‑1 (MQ=255)                 cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                                        <  1:353302/67‑1 (MQ=255)                 cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                             >  1:1445872/1‑65 (MQ=255)                  acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                        >  1:938210/1‑69 (MQ=255)                   cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                       <  1:1085283/69‑1 (MQ=255)                    acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                    >  1:3892751/1‑71 (MQ=255)                    acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                    >  1:1215807/1‑71 (MQ=255)                       ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                                 <  1:1739920/71‑1 (MQ=255)                          tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                    >  1:3322513/1‑65 (MQ=255)                           gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                   <  1:2582517/49‑1 (MQ=255)                           gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                   <  1:368264/49‑1 (MQ=255)                             cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                        <  1:2036055/58‑1 (MQ=255)                             cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                        <  1:3826494/58‑1 (MQ=255)                              gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                            <  1:2685332/66‑1 (MQ=255)                                aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                                <  1:3338305/47‑1 (MQ=255)                                aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                        >  1:74025/1‑71 (MQ=255)                                 aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                                  <  1:3299476/44‑1 (MQ=255)                                 aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                                >  1:2171812/1‑46 (MQ=255)                                  tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                      <  1:3405915/71‑1 (MQ=255)                                  tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                      >  1:477006/1‑71 (MQ=255)                                   cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                     >  1:643085/1‑71 (MQ=255)                                     tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                 >  1:2239041/1‑41 (MQ=255)                                      gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                           >  1:1559300/1‑62 (MQ=255)                                      gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                                  >  1:664640/1‑71 (MQ=255)                                       cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                                 <  1:1122052/71‑1 (MQ=255)                                        aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                <  1:1854168/71‑1 (MQ=255)                                           aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                             >  1:1491341/1‑71 (MQ=255)                                            tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                             >  1:507433/1‑70 (MQ=255)                                            tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                            <  1:589586/71‑1 (MQ=255)                                            tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                            <  1:3760758/71‑1 (MQ=255)                                               gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                         <  1:1494823/71‑1 (MQ=255)                                                cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                        >  1:1326631/1‑71 (MQ=255)                                                 ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                       >  1:3369448/1‑71 (MQ=255)                                                 ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                       >  1:2282189/1‑71 (MQ=255)                                                   aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                    <  1:2972567/40‑1 (MQ=255)                                                   aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                     >  1:290260/1‑71 (MQ=255)                                                     tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                        >  1:3139451/1‑50 (MQ=255)                                                     tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa                   >  1:2096639/1‑71 (MQ=255)                                                     tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa                   >  1:3660091/1‑71 (MQ=255)                                                       cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                      >  1:3594428/1‑50 (MQ=255)                                                       cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                  <  1:1619129/70‑1 (MQ=255)                                                       cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                  <  1:1040486/70‑1 (MQ=255)                                                       cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACg                 >  1:2472892/1‑71 (MQ=255)                                                         aTTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                 <  1:2496046/53‑1 (MQ=255)                                                          ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                                  <  1:66698/51‑1 (MQ=255)                                                          ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt               >  1:960573/1‑70 (MQ=255)                                                          ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt               >  1:2838467/1‑70 (MQ=255)                                                           tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                             <  1:3322401/39‑1 (MQ=255)                                                            ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa             <  1:721548/70‑1 (MQ=255)                                                            ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa             <  1:1305193/70‑1 (MQ=255)                                                            ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt            >  1:110300/1‑71 (MQ=255)                                                             tcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                           >  1:545898/1‑39 (MQ=255)                                                               gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                >  1:1969613/1‑48 (MQ=255)                                                               gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcga         <  1:2385048/71‑1 (MQ=255)                                                                 actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacg       <  1:3109764/71‑1 (MQ=255)                                                                   tactGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                      >  1:3852576/1‑38 (MQ=255)                                                                     ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa     <  1:895279/69‑1 (MQ=255)                                                                     ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAgg   >  1:3032326/1‑71 (MQ=255)                                                                     ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg    <  1:3807875/70‑1 (MQ=255)                                                                      tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                        <  1:2901219/49‑1 (MQ=255)                                                                      tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAggg  >  1:1155576/1‑71 (MQ=255)                                                                       |                                                                      TGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGG  >  minE/132171‑132310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑