Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,135 G→T 18.7% P115P (CCG→CCT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,1350GT18.7% 165.8 / 19.4 64P115P (CCG→CCTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (21/31);  new base T (5/7);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

TTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTT  >  minE/385067‑385205
                                                                    |                                                                      
tttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                                                                      >  1:2037630/1‑71 (MQ=255)
 ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                                                                       <  1:2008806/69‑1 (MQ=255)
 ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGAGGCGCAAATGGGCTACCTGCCggg                                                                      <  1:938978/70‑1 (MQ=255)
    cGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatg                                                                  <  1:3329337/71‑1 (MQ=255)
      gCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                                                <  1:2355728/71‑1 (MQ=255)
        cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATTGGCTACCTGCCGGGGATGATCt                                                              <  1:3008884/71‑1 (MQ=255)
        cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                              <  1:3106573/71‑1 (MQ=255)
        cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                              <  1:2075286/71‑1 (MQ=255)
        cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                              >  1:1832261/1‑71 (MQ=255)
                gAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctgct                                                       <  1:3736903/70‑1 (MQ=255)
                     ggTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTgg                                                 <  1:2535290/71‑1 (MQ=255)
                      gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGcgg                                                  <  1:2764584/69‑3 (MQ=255)
                      gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCgggg                                                >  1:3133188/1‑71 (MQ=255)
                      gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCgggg                                                >  1:696918/1‑71 (MQ=255)
                      gTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGc                                                    <  1:450810/67‑1 (MQ=255)
                          gCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                                            >  1:2151925/1‑71 (MQ=255)
                           cTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTg                                                  <  1:2541821/64‑1 (MQ=255)
                            tggtggGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGtt                                          >  1:1105524/1‑71 (MQ=255)
                                 gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctgc                                                        <  1:532101/52‑1 (MQ=255)
                                 gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGc                                     <  1:3899209/71‑1 (MQ=255)
                                    ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                  >  1:1007658/1‑71 (MQ=255)
                                    ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                  >  1:642165/1‑71 (MQ=255)
                                    ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                  <  1:1456049/71‑1 (MQ=255)
                                    ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                  <  1:887719/71‑1 (MQ=255)
                                     cGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                  <  1:1106663/70‑1 (MQ=255)
                                       gTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgct                                                          <  1:3381243/44‑1 (MQ=255)
                                         aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                <  1:3049909/52‑1 (MQ=255)
                                         aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                <  1:3414683/52‑1 (MQ=255)
                                         aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcgg                             >  1:3593323/1‑71 (MQ=255)
                                          cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgct                                                          >  1:2079447/1‑41 (MQ=255)
                                          cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                            >  1:51912/1‑71 (MQ=255)
                                          cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                            <  1:2969541/71‑1 (MQ=255)
                                            ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                           >  1:2952552/1‑70 (MQ=255)
                                             gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                           <  1:920041/69‑1 (MQ=255)
                                             gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                           >  1:3180320/1‑69 (MQ=255)
                                              ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                        <  1:1723176/69‑1 (MQ=255)
                                              ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                        <  1:2199469/69‑1 (MQ=255)
                                                gcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGa                      <  1:31942/71‑1 (MQ=255)
                                                 cgcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGa                      <  1:353451/70‑1 (MQ=255)
                                                  gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTCGTTCTCGCCGGTGCGGTGCAg                        <  1:2034086/67‑1 (MQ=255)
                                                   cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                   >  1:3917260/1‑71 (MQ=255)
                                                    aaaTGGGCTACCTGCCGGGGATGATCTGGGTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                   >  1:1502356/1‑70 (MQ=255)
                                                    aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCggtgcggtgc                          <  1:1083019/63‑1 (MQ=255)
                                                    aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAg                        <  1:1766098/65‑1 (MQ=255)
                                                    aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                   >  1:2676202/1‑70 (MQ=255)
                                                     gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                 <  1:5334/70‑3 (MQ=255)
                                                     aaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc                                       >  1:3525463/1‑49 (MQ=255)
                                                      aTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcggtgcggtt                           <  1:3770706/60‑2 (MQ=255)
                                                      aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                           <  1:3262511/44‑1 (MQ=255)
                                                      aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                           <  1:245569/44‑1 (MQ=255)
                                                      aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                >  1:2791340/1‑71 (MQ=255)
                                                      aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                >  1:221712/1‑71 (MQ=255)
                                                       tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg               >  1:2352343/1‑71 (MQ=255)
                                                          gCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTg            >  1:21204/1‑71 (MQ=255)
                                                           cTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                    >  1:1403815/1‑62 (MQ=255)
                                                            tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTga           >  1:1253979/1‑69 (MQ=255)
                                                              ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTc                  <  1:2928980/61‑1 (MQ=255)
                                                              ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt        >  1:1932713/1‑71 (MQ=255)
                                                              ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCt          <  1:2920578/69‑1 (MQ=255)
                                                              ccTGCCGGGGATGATATGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt        >  1:5547/1‑71 (MQ=255)
                                                              ccTGCAGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAt                     >  1:1525154/1‑58 (MQ=255)
                                                                 gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt      <  1:3784738/70‑1 (MQ=255)
                                                                 gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt      <  1:1753348/70‑1 (MQ=255)
                                                                  gcGGGGATGATGTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                   <  1:1342292/55‑1 (MQ=255)
                                                                  ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt    <  1:2643513/71‑1 (MQ=255)
                                                                  ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt    >  1:2787022/1‑71 (MQ=255)
                                                                  ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt      <  1:2164562/69‑1 (MQ=255)
                                                                    ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                           <  1:3786238/46‑1 (MQ=255)
                                                                    ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt  <  1:3854765/71‑1 (MQ=255)
                                                                    |                                                                      
TTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTT  >  minE/385067‑385205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: