Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,247	A→G	10.6%	K180K (AAA→AAG)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,247	0	A	G	10.6%	199.5 / 12.5	75	K180K (AAA→AAG)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base A (38/29);  new base G (4/4);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACAC  >  minE/132179‑132315                                                                     |                                                                     atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                                    >  1:312576/1‑71 (MQ=255) atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                                    >  1:524861/1‑71 (MQ=255)  tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                                    >  1:3279009/1‑70 (MQ=255)  tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                                    >  1:1589625/1‑70 (MQ=255)    aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                                 <  1:3378901/71‑1 (MQ=255)    aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                                 <  1:1866093/71‑1 (MQ=255)    aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                                 <  1:3916233/71‑1 (MQ=255)     ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                                 >  1:369444/1‑70 (MQ=255)     ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                                 <  1:895428/70‑1 (MQ=255)     ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                                                >  1:3018776/1‑71 (MQ=255)      cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                               >  1:1094608/1‑71 (MQ=255)      cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                               >  1:3459369/1‑71 (MQ=255)      aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                               >  1:2295944/2‑71 (MQ=255)       aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                               >  1:1704122/1‑70 (MQ=255)        tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                                  >  1:413424/1‑66 (MQ=255)        tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                                    <  1:3129806/64‑1 (MQ=255)        tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                                              >  1:2795204/1‑70 (MQ=255)        tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                             <  1:822055/71‑1 (MQ=255)         cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                                             <  1:353302/67‑1 (MQ=255)         cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                                  >  1:1445872/1‑65 (MQ=255)          acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                             >  1:938210/1‑69 (MQ=255)           cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                            <  1:1085283/69‑1 (MQ=255)            acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                         >  1:3892751/1‑71 (MQ=255)            acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                         >  1:1215807/1‑71 (MQ=255)               ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                                      <  1:1739920/71‑1 (MQ=255)                  tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                         >  1:3322513/1‑65 (MQ=255)                     cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                             <  1:3826494/58‑1 (MQ=255)                     cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                             <  1:2036055/58‑1 (MQ=255)                      gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                                 <  1:2685332/66‑1 (MQ=255)                        aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                                     <  1:3338305/47‑1 (MQ=255)                        aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                             >  1:74025/1‑71 (MQ=255)                         aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                                     >  1:2171812/1‑46 (MQ=255)                          tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                           <  1:3405915/71‑1 (MQ=255)                          tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                           >  1:477006/1‑71 (MQ=255)                           cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                          >  1:643085/1‑71 (MQ=255)                             tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                      >  1:2239041/1‑41 (MQ=255)                              gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                >  1:1559300/1‑62 (MQ=255)                              gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                                       >  1:664640/1‑71 (MQ=255)                               cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                                      <  1:1122052/71‑1 (MQ=255)                                aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                     <  1:1854168/71‑1 (MQ=255)                                   aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                                  >  1:1491341/1‑71 (MQ=255)                                    tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                                  >  1:507433/1‑70 (MQ=255)                                    tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                 <  1:589586/71‑1 (MQ=255)                                    tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                 <  1:3760758/71‑1 (MQ=255)                                       gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                              <  1:1494823/71‑1 (MQ=255)                                        cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                             >  1:1326631/1‑71 (MQ=255)                                         ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                            >  1:2282189/1‑71 (MQ=255)                                         ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                            >  1:3369448/1‑71 (MQ=255)                                           aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                                         <  1:2972567/40‑1 (MQ=255)                                           aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                          >  1:290260/1‑71 (MQ=255)                                             tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                             >  1:3139451/1‑50 (MQ=255)                                             tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa                        >  1:2096639/1‑71 (MQ=255)                                             tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa                        >  1:3660091/1‑71 (MQ=255)                                               cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                           >  1:3594428/1‑50 (MQ=255)                                               cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                       <  1:1619129/70‑1 (MQ=255)                                               cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                       <  1:1040486/70‑1 (MQ=255)                                               cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACg                      >  1:2472892/1‑71 (MQ=255)                                                 aTTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                      <  1:2496046/53‑1 (MQ=255)                                                  ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                                       <  1:66698/51‑1 (MQ=255)                                                  ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt                    >  1:960573/1‑70 (MQ=255)                                                  ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt                    >  1:2838467/1‑70 (MQ=255)                                                   tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                                  <  1:3322401/39‑1 (MQ=255)                                                    ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                  <  1:1305193/70‑1 (MQ=255)                                                    ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                  <  1:721548/70‑1 (MQ=255)                                                    ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                 >  1:110300/1‑71 (MQ=255)                                                     tcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                >  1:545898/1‑39 (MQ=255)                                                       gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                     >  1:1969613/1‑48 (MQ=255)                                                       gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcga              <  1:2385048/71‑1 (MQ=255)                                                         actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacg            <  1:3109764/71‑1 (MQ=255)                                                           tactGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                           >  1:3852576/1‑38 (MQ=255)                                                             ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa          <  1:895279/69‑1 (MQ=255)                                                             ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAgg        >  1:3032326/1‑71 (MQ=255)                                                             ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg         <  1:3807875/70‑1 (MQ=255)                                                              tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                             <  1:2901219/49‑1 (MQ=255)                                                              tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAggg       >  1:1155576/1‑71 (MQ=255)                                                                cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa          <  1:3171546/66‑1 (MQ=255)                                                                 aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa     >  1:1870577/1‑70 (MQ=255)                                                                  aaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTac    <  1:833872/70‑1 (MQ=255)                                                                   aaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                       <  1:3285730/50‑1 (MQ=255)                                                                     aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                              >  1:1998915/1‑41 (MQ=255)                                                                     aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac  >  1:1324391/1‑69 (MQ=255)                                                                     |                                                                     ATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACAC  >  minE/132179‑132315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑