Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,142 A→C 17.9% I118L (ATC→CTC) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,1420AC17.9% 117.9 / 29.5 67I118L (ATC→CTC) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (21/34);  new base C (5/7);  total (26/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

GCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAC  >  minE/385073‑385209
                                                                     |                                                                   
gCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                                                    <  1:2355728/71‑1 (MQ=255)
  cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATTGGCTACCTGCCGGGGATGATCt                                                                  <  1:3008884/71‑1 (MQ=255)
  cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                                  <  1:2075286/71‑1 (MQ=255)
  cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                                  >  1:1832261/1‑71 (MQ=255)
  cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                                  <  1:3106573/71‑1 (MQ=255)
          gAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctgct                                                           <  1:3736903/70‑1 (MQ=255)
               ggTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTgg                                                     <  1:2535290/71‑1 (MQ=255)
                gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGcgg                                                      <  1:2764584/69‑3 (MQ=255)
                gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCgggg                                                    >  1:3133188/1‑71 (MQ=255)
                gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCgggg                                                    >  1:696918/1‑71 (MQ=255)
                gTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGc                                                        <  1:450810/67‑1 (MQ=255)
                    gCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                                                >  1:2151925/1‑71 (MQ=255)
                     cTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTg                                                      <  1:2541821/64‑1 (MQ=255)
                      tggtggGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGtt                                              >  1:1105524/1‑71 (MQ=255)
                           gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctgc                                                            <  1:532101/52‑1 (MQ=255)
                           gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGc                                         <  1:3899209/71‑1 (MQ=255)
                              ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                      >  1:1007658/1‑71 (MQ=255)
                              ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                      >  1:642165/1‑71 (MQ=255)
                              ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                      <  1:887719/71‑1 (MQ=255)
                              ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                      <  1:1456049/71‑1 (MQ=255)
                               cGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                      <  1:1106663/70‑1 (MQ=255)
                                 gTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgct                                                              <  1:3381243/44‑1 (MQ=255)
                                   aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                    <  1:3049909/52‑1 (MQ=255)
                                   aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                    <  1:3414683/52‑1 (MQ=255)
                                   aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcgg                                 >  1:3593323/1‑71 (MQ=255)
                                    cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgct                                                              >  1:2079447/1‑41 (MQ=255)
                                    cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                <  1:2969541/71‑1 (MQ=255)
                                    cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                >  1:51912/1‑71 (MQ=255)
                                      ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                               >  1:2952552/1‑70 (MQ=255)
                                       gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                               >  1:3180320/1‑69 (MQ=255)
                                       gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                               <  1:920041/69‑1 (MQ=255)
                                        ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                            <  1:2199469/69‑1 (MQ=255)
                                        ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                            <  1:1723176/69‑1 (MQ=255)
                                          gcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGa                          <  1:31942/71‑1 (MQ=255)
                                           cgcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGa                          <  1:353451/70‑1 (MQ=255)
                                            gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTCGTTCTCGCCGGTGCGGTGCAg                            <  1:2034086/67‑1 (MQ=255)
                                             cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                       >  1:3917260/1‑71 (MQ=255)
                                              aaaTGGGCTACCTGCCGGGGATGATCTGGGTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                       >  1:1502356/1‑70 (MQ=255)
                                              aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCggtgcggtgc                              <  1:1083019/63‑1 (MQ=255)
                                              aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAg                            <  1:1766098/65‑1 (MQ=255)
                                              aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                       >  1:2676202/1‑70 (MQ=255)
                                               gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                     <  1:5334/70‑3 (MQ=255)
                                               aaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc                                           >  1:3525463/1‑49 (MQ=255)
                                                aTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcggtgcggtt                               <  1:3770706/60‑2 (MQ=255)
                                                aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                               <  1:3262511/44‑1 (MQ=255)
                                                aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                               <  1:245569/44‑1 (MQ=255)
                                                aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                    >  1:2791340/1‑71 (MQ=255)
                                                aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                    >  1:221712/1‑71 (MQ=255)
                                                 tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                   >  1:2352343/1‑71 (MQ=255)
                                                    gCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTg                >  1:21204/1‑71 (MQ=255)
                                                     cTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                        >  1:1403815/1‑62 (MQ=255)
                                                      tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTga               >  1:1253979/1‑69 (MQ=255)
                                                        ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTc                      <  1:2928980/61‑1 (MQ=255)
                                                        ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt            >  1:1932713/1‑71 (MQ=255)
                                                        ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCt              <  1:2920578/69‑1 (MQ=255)
                                                        ccTGCCGGGGATGATATGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt            >  1:5547/1‑71 (MQ=255)
                                                        ccTGCAGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAt                         >  1:1525154/1‑58 (MQ=255)
                                                           gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt          <  1:1753348/70‑1 (MQ=255)
                                                           gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt          <  1:3784738/70‑1 (MQ=255)
                                                            gcGGGGATGATGTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                       <  1:1342292/55‑1 (MQ=255)
                                                            ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt        >  1:2787022/1‑71 (MQ=255)
                                                            ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt        <  1:2643513/71‑1 (MQ=255)
                                                            ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt          <  1:2164562/69‑1 (MQ=255)
                                                              ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                               <  1:3786238/46‑1 (MQ=255)
                                                              ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt      <  1:3854765/71‑1 (MQ=255)
                                                               gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt      <  1:3092309/70‑1 (MQ=255)
                                                                ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                   <  1:2088594/40‑1 (MQ=255)
                                                                ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                   <  1:1541613/40‑1 (MQ=255)
                                                                  atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc  <  1:61701/71‑1 (MQ=255)
                                                                    gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt        <  1:3757285/63‑1 (MQ=255)
                                                                     |                                                                   
GCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAC  >  minE/385073‑385209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: