Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,144 C→G 18.9% I118M (ATC→ATG) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,1440CG18.9% 145.1 / 26.4 69I118M (ATC→ATG) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base C (20/35);  new base G (5/8);  total (26/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

CATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCC  >  minE/385075‑385214
                                                                     |                                                                      
cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATTGGCTACCTGCCGGGGATGATCt                                                                       <  1:3008884/71‑1 (MQ=255)
cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                                       <  1:2075286/71‑1 (MQ=255)
cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                                       <  1:3106573/71‑1 (MQ=255)
cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCt                                                                       >  1:1832261/1‑71 (MQ=255)
        gAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctgct                                                                <  1:3736903/70‑1 (MQ=255)
             ggTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTgg                                                          <  1:2535290/71‑1 (MQ=255)
              gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGcgg                                                           <  1:2764584/69‑3 (MQ=255)
              gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCgggg                                                         >  1:696918/1‑71 (MQ=255)
              gTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCgggg                                                         >  1:3133188/1‑71 (MQ=255)
              gTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGc                                                             <  1:450810/67‑1 (MQ=255)
                  gCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                                                     >  1:2151925/1‑71 (MQ=255)
                   cTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTg                                                           <  1:2541821/64‑1 (MQ=255)
                    tggtggGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGtt                                                   >  1:1105524/1‑71 (MQ=255)
                         gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctgc                                                                 <  1:532101/52‑1 (MQ=255)
                         gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGc                                              <  1:3899209/71‑1 (MQ=255)
                            ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                           >  1:1007658/1‑71 (MQ=255)
                            ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                           >  1:642165/1‑71 (MQ=255)
                            ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                           <  1:1456049/71‑1 (MQ=255)
                            ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                           <  1:887719/71‑1 (MQ=255)
                             cGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                           <  1:1106663/70‑1 (MQ=255)
                               gTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgct                                                                   <  1:3381243/44‑1 (MQ=255)
                                 aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                         <  1:3049909/52‑1 (MQ=255)
                                 aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                         <  1:3414683/52‑1 (MQ=255)
                                 aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcgg                                      >  1:3593323/1‑71 (MQ=255)
                                  cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgct                                                                   >  1:2079447/1‑41 (MQ=255)
                                  cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                     >  1:51912/1‑71 (MQ=255)
                                  cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                     <  1:2969541/71‑1 (MQ=255)
                                    ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                    >  1:2952552/1‑70 (MQ=255)
                                     gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                    >  1:3180320/1‑69 (MQ=255)
                                     gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                    <  1:920041/69‑1 (MQ=255)
                                      ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                                 <  1:2199469/69‑1 (MQ=255)
                                      ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                                 <  1:1723176/69‑1 (MQ=255)
                                        gcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGa                               <  1:31942/71‑1 (MQ=255)
                                         cgcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGa                               <  1:353451/70‑1 (MQ=255)
                                          gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTCGTTCTCGCCGGTGCGGTGCAg                                 <  1:2034086/67‑1 (MQ=255)
                                           cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                            >  1:3917260/1‑71 (MQ=255)
                                            aaaTGGGCTACCTGCCGGGGATGATCTGGGTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                            >  1:1502356/1‑70 (MQ=255)
                                            aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCggtgcggtgc                                   <  1:1083019/63‑1 (MQ=255)
                                            aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAg                                 <  1:1766098/65‑1 (MQ=255)
                                            aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                            >  1:2676202/1‑70 (MQ=255)
                                             gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                          <  1:5334/70‑3 (MQ=255)
                                             aaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc                                                >  1:3525463/1‑49 (MQ=255)
                                              aTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcggtgcggtt                                    <  1:3770706/60‑2 (MQ=255)
                                              aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                                    <  1:245569/44‑1 (MQ=255)
                                              aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                                    <  1:3262511/44‑1 (MQ=255)
                                              aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                         >  1:2791340/1‑71 (MQ=255)
                                              aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                         >  1:221712/1‑71 (MQ=255)
                                               tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                        >  1:2352343/1‑71 (MQ=255)
                                                  gCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTg                     >  1:21204/1‑71 (MQ=255)
                                                   cTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                             >  1:1403815/1‑62 (MQ=255)
                                                    tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTga                    >  1:1253979/1‑69 (MQ=255)
                                                      ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTc                           <  1:2928980/61‑1 (MQ=255)
                                                      ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                 >  1:1932713/1‑71 (MQ=255)
                                                      ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCt                   <  1:2920578/69‑1 (MQ=255)
                                                      ccTGCCGGGGATGATATGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                 >  1:5547/1‑71 (MQ=255)
                                                      ccTGCAGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAt                              >  1:1525154/1‑58 (MQ=255)
                                                         gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt               <  1:1753348/70‑1 (MQ=255)
                                                         gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt               <  1:3784738/70‑1 (MQ=255)
                                                          gcGGGGATGATGTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                            <  1:1342292/55‑1 (MQ=255)
                                                          ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt             >  1:2787022/1‑71 (MQ=255)
                                                          ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt             <  1:2643513/71‑1 (MQ=255)
                                                          ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt               <  1:2164562/69‑1 (MQ=255)
                                                            ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                    <  1:3786238/46‑1 (MQ=255)
                                                            ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt           <  1:3854765/71‑1 (MQ=255)
                                                             gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt           <  1:3092309/70‑1 (MQ=255)
                                                              ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                        <  1:1541613/40‑1 (MQ=255)
                                                              ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                        <  1:2088594/40‑1 (MQ=255)
                                                                atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc       <  1:61701/71‑1 (MQ=255)
                                                                  gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt             <  1:3757285/63‑1 (MQ=255)
                                                                     cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc  >  1:1292221/1‑71 (MQ=255)
                                                                     |                                                                      
CATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCC  >  minE/385075‑385214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: