Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,162 G→C 16.9% V124V (GTG→GTC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,1620GC16.9% 149.5 / 20.7 65V124V (GTG→GTCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (23/31);  new base C (3/8);  total (26/39)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCT  >  minE/385093‑385230
                                                                     |                                                                    
gCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                                                                     >  1:2151925/1‑71 (MQ=255)
  tggtggGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGtt                                                                   >  1:1105524/1‑71 (MQ=255)
       gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGc                                                              <  1:3899209/71‑1 (MQ=255)
          ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                                           >  1:642165/1‑71 (MQ=255)
          ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                                           >  1:1007658/1‑71 (MQ=255)
          ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                                           <  1:887719/71‑1 (MQ=255)
          ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                                           <  1:1456049/71‑1 (MQ=255)
           cGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                                           <  1:1106663/70‑1 (MQ=255)
               aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcgg                                                      >  1:3593323/1‑71 (MQ=255)
                cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                                     <  1:2969541/71‑1 (MQ=255)
                cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                                     >  1:51912/1‑71 (MQ=255)
                  ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                    >  1:2952552/1‑70 (MQ=255)
                   gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                    <  1:920041/69‑1 (MQ=255)
                   gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                    >  1:3180320/1‑69 (MQ=255)
                    ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                                                 <  1:2199469/69‑1 (MQ=255)
                    ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                                                 <  1:1723176/69‑1 (MQ=255)
                      gcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGa                                               <  1:31942/71‑1 (MQ=255)
                       cgcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGa                                               <  1:353451/70‑1 (MQ=255)
                        gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTCGTTCTCGCCGGTGCGGTGCAg                                                 <  1:2034086/67‑1 (MQ=255)
                         cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                            >  1:3917260/1‑71 (MQ=255)
                          aaaTGGGCTACCTGCCGGGGATGATCTGGGTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                            >  1:1502356/1‑70 (MQ=255)
                          aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCggtgcggtgc                                                   <  1:1083019/63‑1 (MQ=255)
                          aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAg                                                 <  1:1766098/65‑1 (MQ=255)
                          aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                            >  1:2676202/1‑70 (MQ=255)
                           gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                          <  1:5334/70‑3 (MQ=255)
                           aaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc                                                                >  1:3525463/1‑49 (MQ=255)
                            aTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcggtgcggtt                                                    <  1:3770706/60‑2 (MQ=255)
                            aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                                                    <  1:245569/44‑1 (MQ=255)
                            aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtggt                                                                    <  1:3262511/44‑1 (MQ=255)
                            aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                                         >  1:221712/1‑71 (MQ=255)
                            aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                                         >  1:2791340/1‑71 (MQ=255)
                             tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                                        >  1:2352343/1‑71 (MQ=255)
                                gCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTg                                     >  1:21204/1‑71 (MQ=255)
                                 cTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                                             >  1:1403815/1‑62 (MQ=255)
                                  tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTga                                    >  1:1253979/1‑69 (MQ=255)
                                    ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTc                                           <  1:2928980/61‑1 (MQ=255)
                                    ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                 >  1:1932713/1‑71 (MQ=255)
                                    ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCt                                   <  1:2920578/69‑1 (MQ=255)
                                    ccTGCCGGGGATGATATGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                 >  1:5547/1‑71 (MQ=255)
                                    ccTGCAGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAt                                              >  1:1525154/1‑58 (MQ=255)
                                       gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt                               <  1:1753348/70‑1 (MQ=255)
                                       gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt                               <  1:3784738/70‑1 (MQ=255)
                                        gcGGGGATGATGTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                            <  1:1342292/55‑1 (MQ=255)
                                        ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                             >  1:2787022/1‑71 (MQ=255)
                                        ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                             <  1:2643513/71‑1 (MQ=255)
                                        ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt                               <  1:2164562/69‑1 (MQ=255)
                                          ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                    <  1:3786238/46‑1 (MQ=255)
                                          ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                           <  1:3854765/71‑1 (MQ=255)
                                           gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                           <  1:3092309/70‑1 (MQ=255)
                                            ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                                        <  1:1541613/40‑1 (MQ=255)
                                            ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                                        <  1:2088594/40‑1 (MQ=255)
                                              atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                       <  1:61701/71‑1 (MQ=255)
                                                gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                             <  1:3757285/63‑1 (MQ=255)
                                                   cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                  >  1:1292221/1‑71 (MQ=255)
                                                      gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg               <  1:1496482/71‑1 (MQ=255)
                                                       ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGtaa              <  1:1499100/71‑3 (MQ=255)
                                                       ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa              <  1:541963/71‑1 (MQ=255)
                                                         gctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAt                                              <  1:3669093/37‑1 (MQ=255)
                                                         gctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                          <  1:3721454/41‑1 (MQ=255)
                                                         gctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                          <  1:1910038/41‑1 (MQ=255)
                                                          ctCGCTGGGGTGGTTCTCGCCTGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                       <  1:3927998/59‑1 (MQ=255)
                                                           tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                                        >  1:3812814/1‑41 (MQ=255)
                                                           tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                                        >  1:1995825/1‑41 (MQ=255)
                                                           tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                             >  1:1666203/1‑52 (MQ=255)
                                                             gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc         >  1:1387997/1‑70 (MQ=255)
                                                             gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc         <  1:1018140/70‑1 (MQ=255)
                                                                 gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                 <  1:568432/42‑1 (MQ=255)
                                                                 gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc     <  1:3735801/70‑1 (MQ=255)
                                                                   gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct  <  1:2587752/71‑1 (MQ=255)
                                                                     |                                                                    
GCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCT  >  minE/385093‑385230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: