Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,165 T→G 15.4% V125V (GTT→GTG cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,1650TG15.4% 117.5 / 24.9 65V125V (GTT→GTGcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (21/34);  new base G (3/7);  total (24/41)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

TGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGT  >  minE/385095‑385234
                                                                      |                                                                     
tggtggGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGtt                                                                       >  1:1105524/1‑71 (MQ=255)
     gggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGc                                                                  <  1:3899209/71‑1 (MQ=255)
        ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                                               >  1:1007658/1‑71 (MQ=255)
        ccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                                               >  1:642165/1‑71 (MQ=255)
        ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                                               <  1:887719/71‑1 (MQ=255)
        ccGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcgg                                                               <  1:1456049/71‑1 (MQ=255)
         cGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcgg                                                               <  1:1106663/70‑1 (MQ=255)
             aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcgg                                                          >  1:3593323/1‑71 (MQ=255)
              cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                                         >  1:51912/1‑71 (MQ=255)
              cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggt                                                         <  1:2969541/71‑1 (MQ=255)
                ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                        >  1:2952552/1‑70 (MQ=255)
                 gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                        <  1:920041/69‑1 (MQ=255)
                 gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                        >  1:3180320/1‑69 (MQ=255)
                  ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                                                     <  1:1723176/69‑1 (MQ=255)
                  ccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAg                                                     <  1:2199469/69‑1 (MQ=255)
                    gcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGa                                                   <  1:31942/71‑1 (MQ=255)
                     cgcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGa                                                   <  1:353451/70‑1 (MQ=255)
                      gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTCGTTCTCGCCGGTGCGGTGCAg                                                     <  1:2034086/67‑1 (MQ=255)
                       cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                >  1:3917260/1‑71 (MQ=255)
                        aaaTGGGCTACCTGCCGGGGATGATCTGGGTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                >  1:1502356/1‑70 (MQ=255)
                        aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCggtgcggtgc                                                       <  1:1083019/63‑1 (MQ=255)
                        aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAg                                                     <  1:1766098/65‑1 (MQ=255)
                        aaaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                >  1:2676202/1‑70 (MQ=255)
                         gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                              <  1:5334/70‑3 (MQ=255)
                         aaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc                                                                    >  1:3525463/1‑49 (MQ=255)
                          aTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcggtgcggtt                                                        <  1:3770706/60‑2 (MQ=255)
                          aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                                             >  1:2791340/1‑71 (MQ=255)
                          aTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCAt                                             >  1:221712/1‑71 (MQ=255)
                           tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                                            >  1:2352343/1‑71 (MQ=255)
                              gCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTg                                         >  1:21204/1‑71 (MQ=255)
                               cTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                                                 >  1:1403815/1‑62 (MQ=255)
                                tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTga                                        >  1:1253979/1‑69 (MQ=255)
                                  ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTc                                               <  1:2928980/61‑1 (MQ=255)
                                  ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                     >  1:1932713/1‑71 (MQ=255)
                                  ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCt                                       <  1:2920578/69‑1 (MQ=255)
                                  ccTGCCGGGGATGATATGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                     >  1:5547/1‑71 (MQ=255)
                                  ccTGCAGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAt                                                  >  1:1525154/1‑58 (MQ=255)
                                     gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt                                   <  1:1753348/70‑1 (MQ=255)
                                     gCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt                                   <  1:3784738/70‑1 (MQ=255)
                                      gcGGGGATGATGTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                <  1:1342292/55‑1 (MQ=255)
                                      ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                 <  1:2643513/71‑1 (MQ=255)
                                      ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                 >  1:2787022/1‑71 (MQ=255)
                                      ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgttt                                   <  1:2164562/69‑1 (MQ=255)
                                        ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                        <  1:3786238/46‑1 (MQ=255)
                                        ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                               <  1:3854765/71‑1 (MQ=255)
                                         gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                               <  1:3092309/70‑1 (MQ=255)
                                          ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                                            <  1:1541613/40‑1 (MQ=255)
                                          ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgc                                                            <  1:2088594/40‑1 (MQ=255)
                                            atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                           <  1:61701/71‑1 (MQ=255)
                                              gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                 <  1:3757285/63‑1 (MQ=255)
                                                 cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                      >  1:1292221/1‑71 (MQ=255)
                                                    gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg                   <  1:1496482/71‑1 (MQ=255)
                                                     ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGtaa                  <  1:1499100/71‑3 (MQ=255)
                                                     ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                  <  1:541963/71‑1 (MQ=255)
                                                       gctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAt                                                  <  1:3669093/37‑1 (MQ=255)
                                                       gctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                              <  1:3721454/41‑1 (MQ=255)
                                                       gctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                              <  1:1910038/41‑1 (MQ=255)
                                                        ctCGCTGGGGTGGTTCTCGCCTGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                           <  1:3927998/59‑1 (MQ=255)
                                                         tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                                            >  1:1995825/1‑41 (MQ=255)
                                                         tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATg                                            >  1:3812814/1‑41 (MQ=255)
                                                         tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                 >  1:1666203/1‑52 (MQ=255)
                                                           gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc             >  1:1387997/1‑70 (MQ=255)
                                                           gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc             <  1:1018140/70‑1 (MQ=255)
                                                               gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                     <  1:568432/42‑1 (MQ=255)
                                                               gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc         <  1:3735801/70‑1 (MQ=255)
                                                                 gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct      <  1:2587752/71‑1 (MQ=255)
                                                                    gtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttg                                  <  1:3948365/40‑1 (MQ=255)
                                                                     ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt              <  1:1524803/59‑1 (MQ=255)
                                                                     ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTg     <  1:2357919/68‑1 (MQ=255)
                                                                     ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt  <  1:1984576/71‑1 (MQ=255)
                                                                      |                                                                     
TGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGT  >  minE/385095‑385234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: