Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,202 G→A 13.9% V138I (GTT→ATT) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2020GA13.9% 106.8 / 9.0 43V138I (GTT→ATT) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (12/25);  new base A (3/3);  total (15/28)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGG  >  minE/385133‑385271
                                                                     |                                                                      
ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       >  1:2787022/1‑71 (MQ=255)
ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       <  1:2643513/71‑1 (MQ=255)
  ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                                     <  1:3854765/71‑1 (MQ=255)
   gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                                     <  1:3092309/70‑1 (MQ=255)
      atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                 <  1:61701/71‑1 (MQ=255)
        gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       <  1:3757285/63‑1 (MQ=255)
           cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                                                            >  1:1292221/1‑71 (MQ=255)
              gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg                                                         <  1:1496482/71‑1 (MQ=255)
               ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGtaa                                                        <  1:1499100/71‑3 (MQ=255)
               ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                        <  1:541963/71‑1 (MQ=255)
                  ctCGCTGGGGTGGTTCTCGCCTGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                 <  1:3927998/59‑1 (MQ=255)
                   tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       >  1:1666203/1‑52 (MQ=255)
                     gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                   <  1:1018140/70‑1 (MQ=255)
                     gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                   >  1:1387997/1‑70 (MQ=255)
                         gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                              <  1:3735801/70‑1 (MQ=255)
                           gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgc‑tcgct                                           <  1:2587752/71‑1 (MQ=255)
                              gtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttg                                                                        <  1:3948365/40‑1 (MQ=255)
                               ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                    <  1:1524803/59‑1 (MQ=255)
                               ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTg                                          <  1:2357919/68‑1 (MQ=255)
                               ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                       <  1:1984576/71‑1 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttg                                                                        >  1:2178103/1‑37 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTggg                                        >  1:223380/1‑68 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                       >  1:1024244/1‑69 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                       >  1:1782952/1‑69 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                       >  1:2163028/1‑69 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTg                                      <  1:1044657/70‑1 (MQ=255)
                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                     <  1:1268447/68‑1 (MQ=255)
                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                     <  1:1010090/68‑1 (MQ=255)
                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                     <  1:3016120/68‑1 (MQ=255)
                                     ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTg                                 >  1:1857168/1‑71 (MQ=255)
                                      cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTgg                                <  1:3503223/71‑1 (MQ=255)
                                             gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                              <  1:73588/50‑1 (MQ=255)
                                               gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag                          >  1:3469037/1‑68 (MQ=255)
                                                cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                      <  1:3604802/71‑1 (MQ=255)
                                                cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAaagaa                        >  1:2566236/1‑69 (MQ=255)
                                                    aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                  >  1:1667077/1‑71 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                 >  1:2983340/1‑71 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:150903/71‑1 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:1753496/71‑1 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:3428842/71‑1 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:2382705/71‑1 (MQ=255)
                                                        taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac              >  1:3188782/2‑69 (MQ=255)
                                                        taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac              >  1:1700810/2‑69 (MQ=255)
                                                        taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                <  1:122186/68‑1 (MQ=255)
                                                        cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc              >  1:724233/1‑71 (MQ=255)
                                                          tGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc             <  1:766422/70‑1 (MQ=255)
                                                             tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:2645659/63‑1 (MQ=255)
                                                             tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                  <  1:38885/62‑1 (MQ=255)
                                                               cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCTCTGGGTGAGCTGGTCAAAGAAGAGATg                  <  1:3191507/60‑1 (MQ=255)
                                                               cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                  <  1:3880871/60‑1 (MQ=255)
                                                               cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc       >  1:1328731/1‑71 (MQ=255)
                                                                tgtttgttTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                      >  1:3225604/1‑55 (MQ=255)
                                                                 gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg     >  1:2727909/1‑71 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                    >  1:737161/1‑55 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:2236217/58‑1 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc      <  1:3240845/69‑1 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg    <  1:1378872/71‑1 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                >  1:3492533/1‑57 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                >  1:2850384/1‑57 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg  >  1:1070097/1‑71 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg  >  1:3356221/1‑71 (MQ=255)
                                                                     |                                                                      
CCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGG  >  minE/385133‑385271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑