Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,202 G→A 13.9% V138I (GTT→ATT) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2020GA13.9% 106.8 / 9.0 43V138I (GTT→ATT) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (12/25);  new base A (3/3);  total (15/28)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGG  >  minE/385133‑385271
                                                                     |                                                                      
ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       >  1:2787022/1‑71 (MQ=255)
ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       <  1:2643513/71‑1 (MQ=255)
  ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                                     <  1:3854765/71‑1 (MQ=255)
   gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                                     <  1:3092309/70‑1 (MQ=255)
      atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                 <  1:61701/71‑1 (MQ=255)
        gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       <  1:3757285/63‑1 (MQ=255)
           cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                                                            >  1:1292221/1‑71 (MQ=255)
              gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg                                                         <  1:1496482/71‑1 (MQ=255)
               ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGtaa                                                        <  1:1499100/71‑3 (MQ=255)
               ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                        <  1:541963/71‑1 (MQ=255)
                  ctCGCTGGGGTGGTTCTCGCCTGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                 <  1:3927998/59‑1 (MQ=255)
                   tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                                       >  1:1666203/1‑52 (MQ=255)
                     gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                   <  1:1018140/70‑1 (MQ=255)
                     gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                   >  1:1387997/1‑70 (MQ=255)
                         gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                              <  1:3735801/70‑1 (MQ=255)
                           gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                                           <  1:2587752/71‑1 (MQ=255)
                              gtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttg                                                                        <  1:3948365/40‑1 (MQ=255)
                               ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                    <  1:1524803/59‑1 (MQ=255)
                               ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTg                                          <  1:2357919/68‑1 (MQ=255)
                               ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                       <  1:1984576/71‑1 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttg                                                                        >  1:2178103/1‑37 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTggg                                        >  1:223380/1‑68 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                       >  1:1024244/1‑69 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                       >  1:1782952/1‑69 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                       >  1:2163028/1‑69 (MQ=255)
                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                                      <  1:1044657/70‑1 (MQ=255)
                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                     <  1:1268447/68‑1 (MQ=255)
                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                     <  1:1010090/68‑1 (MQ=255)
                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                     <  1:3016120/68‑1 (MQ=255)
                                     ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg                                 >  1:1857168/1‑71 (MQ=255)
                                      cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTgg                                <  1:3503223/71‑1 (MQ=255)
                                             gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                              <  1:73588/50‑1 (MQ=255)
                                               gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                          >  1:3469037/1‑68 (MQ=255)
                                                cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                      <  1:3604802/71‑1 (MQ=255)
                                                cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAaagaa                        >  1:2566236/1‑69 (MQ=255)
                                                    aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                  >  1:1667077/1‑71 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                 >  1:2983340/1‑71 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:150903/71‑1 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:1753496/71‑1 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:3428842/71‑1 (MQ=255)
                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:2382705/71‑1 (MQ=255)
                                                        taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac              >  1:3188782/2‑69 (MQ=255)
                                                        taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac              >  1:1700810/2‑69 (MQ=255)
                                                        taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                <  1:122186/68‑1 (MQ=255)
                                                        cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc              >  1:724233/1‑71 (MQ=255)
                                                          tGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc             <  1:766422/70‑1 (MQ=255)
                                                             tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:2645659/63‑1 (MQ=255)
                                                             tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                  <  1:38885/62‑1 (MQ=255)
                                                               cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCTCTGGGTGAGCTGGTCAAAGAAGAGATg                  <  1:3191507/60‑1 (MQ=255)
                                                               cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                  <  1:3880871/60‑1 (MQ=255)
                                                               cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc       >  1:1328731/1‑71 (MQ=255)
                                                                tgtttgttTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                      >  1:3225604/1‑55 (MQ=255)
                                                                 gtttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg     >  1:2727909/1‑71 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                    >  1:737161/1‑55 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                 <  1:2236217/58‑1 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc      <  1:3240845/69‑1 (MQ=255)
                                                                  tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg    <  1:1378872/71‑1 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                >  1:3492533/1‑57 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                >  1:2850384/1‑57 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg  >  1:1070097/1‑71 (MQ=255)
                                                                    tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg  >  1:3356221/1‑71 (MQ=255)
                                                                     |                                                                      
CCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGG  >  minE/385133‑385271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: