Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,204 T→C 13.9% V138V (GTT→GTC) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2040TC13.9% 104.8 / 9.3 43V138V (GTT→GTC) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (12/25);  new base C (3/3);  total (15/28)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

GGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGT  >  minE/385135‑385272
                                                                     |                                                                     
ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                                      <  1:3854765/71‑1 (MQ=255)
 gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                                      <  1:3092309/70‑1 (MQ=255)
    atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                  <  1:61701/71‑1 (MQ=255)
         cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                                                             >  1:1292221/1‑71 (MQ=255)
            gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg                                                          <  1:1496482/71‑1 (MQ=255)
             ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGtaa                                                         <  1:1499100/71‑3 (MQ=255)
             ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                         <  1:541963/71‑1 (MQ=255)
                ctCGCTGGGGTGGTTCTCGCCTGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                  <  1:3927998/59‑1 (MQ=255)
                   gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                    <  1:1018140/70‑1 (MQ=255)
                   gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                    >  1:1387997/1‑70 (MQ=255)
                       gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                               <  1:3735801/70‑1 (MQ=255)
                         gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                                            <  1:2587752/71‑1 (MQ=255)
                             ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                     <  1:1524803/59‑1 (MQ=255)
                             ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTg                                           <  1:2357919/68‑1 (MQ=255)
                             ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                        <  1:1984576/71‑1 (MQ=255)
                               ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTggg                                         >  1:223380/1‑68 (MQ=255)
                               ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                        >  1:1782952/1‑69 (MQ=255)
                               ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                        >  1:2163028/1‑69 (MQ=255)
                               ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                        >  1:1024244/1‑69 (MQ=255)
                               ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                                       <  1:1044657/70‑1 (MQ=255)
                                  gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                      <  1:1010090/68‑1 (MQ=255)
                                  gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                      <  1:1268447/68‑1 (MQ=255)
                                  gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                      <  1:3016120/68‑1 (MQ=255)
                                   ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg                                  >  1:1857168/1‑71 (MQ=255)
                                    cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTgg                                 <  1:3503223/71‑1 (MQ=255)
                                           gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                               <  1:73588/50‑1 (MQ=255)
                                             gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                           >  1:3469037/1‑68 (MQ=255)
                                              cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                       <  1:3604802/71‑1 (MQ=255)
                                              cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAaagaa                         >  1:2566236/1‑69 (MQ=255)
                                                  aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                   >  1:1667077/1‑71 (MQ=255)
                                                   tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                  >  1:2983340/1‑71 (MQ=255)
                                                   tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                  <  1:3428842/71‑1 (MQ=255)
                                                   tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                  <  1:2382705/71‑1 (MQ=255)
                                                   tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                  <  1:150903/71‑1 (MQ=255)
                                                   tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                  <  1:1753496/71‑1 (MQ=255)
                                                      taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac               >  1:3188782/2‑69 (MQ=255)
                                                      taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac               >  1:1700810/2‑69 (MQ=255)
                                                      taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                 <  1:122186/68‑1 (MQ=255)
                                                      cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc               >  1:724233/1‑71 (MQ=255)
                                                        tGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc              <  1:766422/70‑1 (MQ=255)
                                                           tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                  <  1:2645659/63‑1 (MQ=255)
                                                           tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                   <  1:38885/62‑1 (MQ=255)
                                                             cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCTCTGGGTGAGCTGGTCAAAGAAGAGATg                   <  1:3191507/60‑1 (MQ=255)
                                                             cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                   <  1:3880871/60‑1 (MQ=255)
                                                             cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc        >  1:1328731/1‑71 (MQ=255)
                                                              tgtttgttTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                       >  1:3225604/1‑55 (MQ=255)
                                                               gtttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg      >  1:2727909/1‑71 (MQ=255)
                                                                tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                     >  1:737161/1‑55 (MQ=255)
                                                                tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                  <  1:2236217/58‑1 (MQ=255)
                                                                tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc       <  1:3240845/69‑1 (MQ=255)
                                                                tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg     <  1:1378872/71‑1 (MQ=255)
                                                                  tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                 >  1:3492533/1‑57 (MQ=255)
                                                                  tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                 >  1:2850384/1‑57 (MQ=255)
                                                                  tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg   >  1:3356221/1‑71 (MQ=255)
                                                                  tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg   >  1:1070097/1‑71 (MQ=255)
                                                                    tttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt  <  1:3695223/70‑1 (MQ=255)
                                                                     |                                                                     
GGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGT  >  minE/385135‑385272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: