| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 138,390 | A→G | 7.9% | S593P (TCA→CCA) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 138,390 | 0 | A | G | 7.9% | 213.5 / 7.3 | 76 | S593P (TCA→CCA) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (34/36); new base G (3/3); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTG > minE/138320‑138460 | ctgctAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGa < 1:1006760/71‑1 (MQ=255) ctAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGGAg > 1:1302196/1‑70 (MQ=255) ctAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAg < 1:1386946/70‑1 (MQ=255) tAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAg < 1:3002416/69‑1 (MQ=255) aCGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACg < 1:1064727/71‑1 (MQ=255) ttGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACg < 1:3333057/68‑1 (MQ=255) tGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACgttgt > 1:615209/1‑71 (MQ=255) aGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACgttgtt < 1:340429/70‑1 (MQ=255) aGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTc < 1:2568030/71‑1 (MQ=255) gTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACgttgtt > 1:3330556/1‑69 (MQ=255) gTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCa < 1:3764596/71‑1 (MQ=255) ttGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGt < 1:1752647/60‑1 (MQ=255) ttGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAg > 1:2500014/1‑71 (MQ=255) ggTTATTACCCTAAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGATCAGTg > 1:3301964/1‑71 (MQ=255) ttattaCCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACg < 1:3465801/59‑1 (MQ=255) ttattaCCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACg < 1:3227069/59‑1 (MQ=255) ttattaCCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACg < 1:3784008/59‑1 (MQ=255) aCCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAg > 1:2994735/1‑69 (MQ=255) cccTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGt < 1:3820370/69‑1 (MQ=255) ccTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAt < 1:193147/71‑1 (MQ=255) ccTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAt < 1:1192474/71‑1 (MQ=255) ttAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACgttgtt < 1:3890438/55‑1 (MQ=255) ttAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAt > 1:943227/1‑69 (MQ=255) tAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAtttt > 1:1621286/1‑71 (MQ=255) aaaGTCACTGCTTATTTGAGTATGAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAttttt > 1:2547254/1‑71 (MQ=255) aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGaa > 1:1265277/1‑43 (MQ=255) aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGGAGTACGTTGTTCAGTGCCAAGTAAttt < 1:2578607/69‑1 (MQ=255) aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGGAGTACGTTGTTCAGTGCCAAGTAAttt < 1:514565/69‑1 (MQ=255) aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGGAGTACGTTGTTCAGTGCCAAGTAAttt < 1:1447476/69‑1 (MQ=255) aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAttttt > 1:3806254/1‑71 (MQ=255) aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAttttt > 1:3504071/1‑71 (MQ=255) aaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCa > 1:3440942/1‑54 (MQ=255) aGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCa < 1:992653/71‑1 (MQ=255) aGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCa < 1:3674709/71‑1 (MQ=255) tCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCa < 1:3571074/51‑1 (MQ=255) tCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCa < 1:2898386/51‑1 (MQ=255) cACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCa > 1:642469/1‑68 (MQ=255) aCTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAt > 1:779731/1‑61 (MQ=255) cTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAt < 1:3125554/60‑1 (MQ=255) cTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCa > 1:1708467/1‑66 (MQ=255) tGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAg > 1:300446/1‑48 (MQ=255) gCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTc < 1:2594728/63‑1 (MQ=255) cTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGaa < 1:2887390/70‑1 (MQ=255) cTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAAt > 1:2756936/1‑71 (MQ=255) cTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAAt < 1:3740775/71‑1 (MQ=255) ttATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAAt < 1:3267211/64‑1 (MQ=255) ttATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATg > 1:2175567/1‑71 (MQ=255) aTTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATg < 1:1127118/69‑1 (MQ=255) aTTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATg < 1:1550251/69‑1 (MQ=255) tttGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCa < 1:3530143/41‑1 (MQ=255) tttGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGt < 1:774434/70‑1 (MQ=255) tGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGt > 1:870520/1‑41 (MQ=255) tGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGt > 1:20871/1‑41 (MQ=255) tGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGa > 1:2384632/1‑70 (MQ=255) gTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAAc > 1:1995350/1‑70 (MQ=255) gTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACt < 1:1645564/71‑1 (MQ=255) gTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACt < 1:3855869/71‑1 (MQ=255) gTATCAATACTCTGGAAACCCGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAAc > 1:1987171/1‑70 (MQ=255) aTCAATACTCTGGAAACCTGAAGTACGTTGTTCAg < 1:1799176/35‑1 (MQ=255) cAATACTCTGGAAACCTGAAGTACGTTGTTCAGTg > 1:589498/1‑35 (MQ=255) aaTACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGa > 1:2080441/1‑70 (MQ=255) aTACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCaa < 1:1481567/37‑1 (MQ=255) aTACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCaa < 1:313916/37‑1 (MQ=255) tACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATa > 1:3491513/1‑70 (MQ=255) aCTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGt > 1:1780574/1‑37 (MQ=255) aCTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGt > 1:1607216/1‑37 (MQ=255) aCTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGa > 1:2169555/1‑52 (MQ=255) tGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTc < 1:2099949/41‑1 (MQ=255) tGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGc > 1:460578/1‑70 (MQ=255) aaCCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGaaa > 1:2395581/1‑53 (MQ=255) aaCCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGaaa > 1:375659/1‑53 (MQ=255) cTGGAGTACGTTGTTCAGTGCCAAGTAATTTTTCaa > 1:564797/1‑36 (MQ=255) cTGGAGTACGTTGTTCAGTGCCAAGTAATTTTTCaa > 1:1847238/1‑36 (MQ=255) cTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCg > 1:1356001/1‑68 (MQ=255) cTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGt > 1:1048384/1‑69 (MQ=255) cTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCa < 1:948056/71‑1 (MQ=255) tGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTgg < 1:165582/39‑1 (MQ=255) gAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAg > 1:2296904/1‑70 (MQ=255) aaGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAgtg > 1:2427264/1‑71 (MQ=255) | CTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTG > minE/138320‑138460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |