Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,208 A→T 9.8% T140S (ACG→TCG) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2080AT 9.8% 166.1 / 9.6 61T140S (ACG→TCG) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (23/32);  new base T (3/3);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

ATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATT  >  minE/385139‑385276
                                                                     |                                                                     
atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                      <  1:61701/71‑1 (MQ=255)
     cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                                                                 >  1:1292221/1‑71 (MQ=255)
        gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg                                                              <  1:1496482/71‑1 (MQ=255)
         ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGtaa                                                             <  1:1499100/71‑3 (MQ=255)
         ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                             <  1:541963/71‑1 (MQ=255)
            ctCGCTGGGGTGGTTCTCGCCTGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAc                                                                      <  1:3927998/59‑1 (MQ=255)
               gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                        <  1:1018140/70‑1 (MQ=255)
               gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                        >  1:1387997/1‑70 (MQ=255)
                   gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                                   <  1:3735801/70‑1 (MQ=255)
                     gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                                                <  1:2587752/71‑1 (MQ=255)
                         ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                         <  1:1524803/59‑1 (MQ=255)
                         ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTg                                               <  1:2357919/68‑1 (MQ=255)
                         ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                            <  1:1984576/71‑1 (MQ=255)
                           ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTggg                                             >  1:223380/1‑68 (MQ=255)
                           ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                            >  1:1024244/1‑69 (MQ=255)
                           ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                            >  1:1782952/1‑69 (MQ=255)
                           ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                            >  1:2163028/1‑69 (MQ=255)
                           ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                                           <  1:1044657/70‑1 (MQ=255)
                              gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                          <  1:1268447/68‑1 (MQ=255)
                              gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                          <  1:1010090/68‑1 (MQ=255)
                              gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                          <  1:3016120/68‑1 (MQ=255)
                               ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg                                      >  1:1857168/1‑71 (MQ=255)
                                cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTgg                                     <  1:3503223/71‑1 (MQ=255)
                                       gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                                   <  1:73588/50‑1 (MQ=255)
                                         gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                               >  1:3469037/1‑68 (MQ=255)
                                          cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                           <  1:3604802/71‑1 (MQ=255)
                                          cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAaagaa                             >  1:2566236/1‑69 (MQ=255)
                                              aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                       >  1:1667077/1‑71 (MQ=255)
                                               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                      >  1:2983340/1‑71 (MQ=255)
                                               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                      <  1:2382705/71‑1 (MQ=255)
                                               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                      <  1:150903/71‑1 (MQ=255)
                                               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                      <  1:1753496/71‑1 (MQ=255)
                                               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                      <  1:3428842/71‑1 (MQ=255)
                                                  taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                   >  1:1700810/2‑69 (MQ=255)
                                                  taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                   >  1:3188782/2‑69 (MQ=255)
                                                  taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                     <  1:122186/68‑1 (MQ=255)
                                                  cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                   >  1:724233/1‑71 (MQ=255)
                                                    tGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                  <  1:766422/70‑1 (MQ=255)
                                                       tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                      <  1:2645659/63‑1 (MQ=255)
                                                       tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                       <  1:38885/62‑1 (MQ=255)
                                                         cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCTCTGGGTGAGCTGGTCAAAGAAGAGATg                       <  1:3191507/60‑1 (MQ=255)
                                                         cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                       <  1:3880871/60‑1 (MQ=255)
                                                         cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc            >  1:1328731/1‑71 (MQ=255)
                                                          tgtttgttTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                           >  1:3225604/1‑55 (MQ=255)
                                                           gtttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg          >  1:2727909/1‑71 (MQ=255)
                                                            tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                         >  1:737161/1‑55 (MQ=255)
                                                            tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                      <  1:2236217/58‑1 (MQ=255)
                                                            tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc           <  1:3240845/69‑1 (MQ=255)
                                                            tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg         <  1:1378872/71‑1 (MQ=255)
                                                              tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                     >  1:2850384/1‑57 (MQ=255)
                                                              tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                     >  1:3492533/1‑57 (MQ=255)
                                                              tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg       >  1:3356221/1‑71 (MQ=255)
                                                              tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg       >  1:1070097/1‑71 (MQ=255)
                                                                tttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt      <  1:3695223/70‑1 (MQ=255)
                                                                  tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGAt                        <  1:729648/50‑1 (MQ=255)
                                                                  tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                 >  1:2277568/1‑57 (MQ=255)
                                                                  tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa    <  1:2329850/70‑1 (MQ=255)
                                                                   cTACGCGCCGTGACGGTCGCTCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt  >  1:1979833/1‑71 (MQ=255)
                                                                   cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt  >  1:2331255/1‑71 (MQ=255)
                                                                   cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt  >  1:1375815/1‑71 (MQ=255)
                                                                   cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt   <  1:3189982/70‑1 (MQ=255)
                                                                     aCGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                       <  1:851634/48‑1 (MQ=255)
                                                                     |                                                                     
ATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATT  >  minE/385139‑385276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: