Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,217 G→A 8.3% D143N (GAC→AAC) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2170GA 8.3% 177.4 / 5.2 60D143N (GAC→AAC) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (22/33);  new base A (3/2);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

GCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGG  >  minE/385147‑385283
                                                                      |                                                                   
gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg                                                                     <  1:1496482/71‑1 (MQ=255)
 ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                                    <  1:541963/71‑1 (MQ=255)
       gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                               <  1:1018140/70‑1 (MQ=255)
       gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                               >  1:1387997/1‑70 (MQ=255)
           gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                                          <  1:3735801/70‑1 (MQ=255)
             gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgc‑tcgct                                                       <  1:2587752/71‑1 (MQ=255)
                 ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                                <  1:1524803/59‑1 (MQ=255)
                 ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTg                                                      <  1:2357919/68‑1 (MQ=255)
                 ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                   <  1:1984576/71‑1 (MQ=255)
                   ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTggg                                                    >  1:223380/1‑68 (MQ=255)
                   ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                   >  1:2163028/1‑69 (MQ=255)
                   ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                   >  1:1782952/1‑69 (MQ=255)
                   ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                   >  1:1024244/1‑69 (MQ=255)
                   ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTg                                                  <  1:1044657/70‑1 (MQ=255)
                      gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                 <  1:3016120/68‑1 (MQ=255)
                      gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                 <  1:1010090/68‑1 (MQ=255)
                      gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                 <  1:1268447/68‑1 (MQ=255)
                       ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTg                                             >  1:1857168/1‑71 (MQ=255)
                        cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTgg                                            <  1:3503223/71‑1 (MQ=255)
                               gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                                          <  1:73588/50‑1 (MQ=255)
                                 gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag                                      >  1:3469037/1‑68 (MQ=255)
                                  cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                  <  1:3604802/71‑1 (MQ=255)
                                  cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAaagaa                                    >  1:2566236/1‑69 (MQ=255)
                                      aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                              >  1:1667077/1‑71 (MQ=255)
                                       tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                             >  1:2983340/1‑71 (MQ=255)
                                       tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                             <  1:3428842/71‑1 (MQ=255)
                                       tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                             <  1:2382705/71‑1 (MQ=255)
                                       tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                             <  1:150903/71‑1 (MQ=255)
                                       tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                             <  1:1753496/71‑1 (MQ=255)
                                          taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                          >  1:3188782/2‑69 (MQ=255)
                                          taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                          >  1:1700810/2‑69 (MQ=255)
                                          taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                            <  1:122186/68‑1 (MQ=255)
                                          cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                          >  1:724233/1‑71 (MQ=255)
                                            tGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                         <  1:766422/70‑1 (MQ=255)
                                               tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                             <  1:2645659/63‑1 (MQ=255)
                                               tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                              <  1:38885/62‑1 (MQ=255)
                                                 cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCTCTGGGTGAGCTGGTCAAAGAAGAGATg                              <  1:3191507/60‑1 (MQ=255)
                                                 cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                              <  1:3880871/60‑1 (MQ=255)
                                                 cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                   >  1:1328731/1‑71 (MQ=255)
                                                  tgtttgttTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                  >  1:3225604/1‑55 (MQ=255)
                                                   gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                 >  1:2727909/1‑71 (MQ=255)
                                                    tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                >  1:737161/1‑55 (MQ=255)
                                                    tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                             <  1:2236217/58‑1 (MQ=255)
                                                    tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc                  <  1:3240845/69‑1 (MQ=255)
                                                    tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg                <  1:1378872/71‑1 (MQ=255)
                                                      tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                            >  1:2850384/1‑57 (MQ=255)
                                                      tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                            >  1:3492533/1‑57 (MQ=255)
                                                      tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg              >  1:1070097/1‑71 (MQ=255)
                                                      tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg              >  1:3356221/1‑71 (MQ=255)
                                                        tttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt             <  1:3695223/70‑1 (MQ=255)
                                                          tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                               <  1:729648/50‑1 (MQ=255)
                                                          tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                        >  1:2277568/1‑57 (MQ=255)
                                                          tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa           <  1:2329850/70‑1 (MQ=255)
                                                           cTACGCGCCGTGACGGTCGC‑TCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt         >  1:1979833/1‑71 (MQ=255)
                                                           cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt         >  1:1375815/1‑71 (MQ=255)
                                                           cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt         >  1:2331255/1‑71 (MQ=255)
                                                           cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt          <  1:3189982/70‑1 (MQ=255)
                                                             aCGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                              <  1:851634/48‑1 (MQ=255)
                                                              cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc       <  1:548553/70‑1 (MQ=255)
                                                              cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc       <  1:1671666/70‑1 (MQ=255)
                                                                  ccGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg  <  1:1977237/71‑1 (MQ=255)
                                                                      gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                >  1:2444047/1‑37 (MQ=255)
                                                                      gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                               <  1:1411758/38‑1 (MQ=255)
                                                                      |                                                                   
GCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGG  >  minE/385147‑385283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑