Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,228 G→T 10.7% S146S (TCG→TCT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2280GT10.7% 177.0 / 5.6 56S146S (TCG→TCTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base T (3/3);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGA  >  minE/385160‑385298
                                                                     |                                                                      
gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                                                                      <  1:2587752/71‑1 (MQ=255)
    ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTg                                                                     <  1:2357919/68‑1 (MQ=255)
    ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  <  1:1984576/71‑1 (MQ=255)
      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTggg                                                                   >  1:223380/1‑68 (MQ=255)
      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  >  1:1782952/1‑69 (MQ=255)
      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  >  1:2163028/1‑69 (MQ=255)
      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  >  1:1024244/1‑69 (MQ=255)
      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                                                                 <  1:1044657/70‑1 (MQ=255)
         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                                                <  1:1268447/68‑1 (MQ=255)
         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                                                <  1:3016120/68‑1 (MQ=255)
         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                                                <  1:1010090/68‑1 (MQ=255)
          ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg                                                            >  1:1857168/1‑71 (MQ=255)
           cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTgg                                                           <  1:3503223/71‑1 (MQ=255)
                    gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                                                     >  1:3469037/1‑68 (MQ=255)
                     cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                                                 <  1:3604802/71‑1 (MQ=255)
                     cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAaagaa                                                   >  1:2566236/1‑69 (MQ=255)
                         aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             >  1:1667077/1‑71 (MQ=255)
                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                                            >  1:2983340/1‑71 (MQ=255)
                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:3428842/71‑1 (MQ=255)
                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2382705/71‑1 (MQ=255)
                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:1753496/71‑1 (MQ=255)
                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:150903/71‑1 (MQ=255)
                             taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                         >  1:1700810/2‑69 (MQ=255)
                             taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                         >  1:3188782/2‑69 (MQ=255)
                             taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                                           <  1:122186/68‑1 (MQ=255)
                             cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                                         >  1:724233/1‑71 (MQ=255)
                               tGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                                        <  1:766422/70‑1 (MQ=255)
                                  tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2645659/63‑1 (MQ=255)
                                  tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:38885/62‑1 (MQ=255)
                                    cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCTCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:3191507/60‑1 (MQ=255)
                                    cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:3880871/60‑1 (MQ=255)
                                    cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                                  >  1:1328731/1‑71 (MQ=255)
                                     tgtttgttTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                                                 >  1:3225604/1‑55 (MQ=255)
                                      gtttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                                >  1:2727909/1‑71 (MQ=255)
                                       tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                                               >  1:737161/1‑55 (MQ=255)
                                       tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2236217/58‑1 (MQ=255)
                                       tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc                                 <  1:3240845/69‑1 (MQ=255)
                                       tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg                               <  1:1378872/71‑1 (MQ=255)
                                         tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                           >  1:3492533/1‑57 (MQ=255)
                                         tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                           >  1:2850384/1‑57 (MQ=255)
                                         tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                             >  1:3356221/1‑71 (MQ=255)
                                         tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                             >  1:1070097/1‑71 (MQ=255)
                                           tttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt                            <  1:3695223/70‑1 (MQ=255)
                                             tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                              <  1:729648/50‑1 (MQ=255)
                                             tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                                       >  1:2277568/1‑57 (MQ=255)
                                             tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa                          <  1:2329850/70‑1 (MQ=255)
                                              cTACGCGCCGTGACGGTCGCTCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:1979833/1‑71 (MQ=255)
                                              cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:1375815/1‑71 (MQ=255)
                                              cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:2331255/1‑71 (MQ=255)
                                              cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                         <  1:3189982/70‑1 (MQ=255)
                                                aCGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:851634/48‑1 (MQ=255)
                                                 cgcgCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                      <  1:548553/70‑1 (MQ=255)
                                                 cgcgCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                      <  1:1671666/70‑1 (MQ=255)
                                                     ccGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg                 <  1:1977237/71‑1 (MQ=255)
                                                         gACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                                               >  1:2444047/1‑37 (MQ=255)
                                                         gACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                              <  1:1411758/38‑1 (MQ=255)
                                                          aCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGcc            >  1:383128/1‑71 (MQ=255)
                                                              tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATgggg                                          >  1:3600125/1‑37 (MQ=255)
                                                              tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt        >  1:1313037/1‑71 (MQ=255)
                                                              tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt        <  1:2502337/71‑1 (MQ=255)
                                                                gctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt      <  1:1056735/71‑1 (MQ=255)
                                                                gctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt      <  1:1779906/71‑1 (MQ=255)
                                                                 ctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        <  1:1563325/52‑1 (MQ=255)
                                                                     gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                         >  1:490806/1‑48 (MQ=255)
                                                                     gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGTTGGCCTGCTTTATGa  >  1:2309060/1‑71 (MQ=255)
                                                                     |                                                                      
GTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGA  >  minE/385160‑385298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: