Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,231 G→T 9.6% L147L (CTG→CTT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2310GT 9.6% 197.4 / 4.7 62L147L (CTG→CTTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (25/31);  new base T (4/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGA  >  minE/385164‑385298
                                                                    |                                                                   
ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTg                                                                     <  1:2357919/68‑1 (MQ=255)
ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  <  1:1984576/71‑1 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTggg                                                                   >  1:223380/1‑68 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  >  1:1024244/1‑69 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  >  1:1782952/1‑69 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                                                                  >  1:2163028/1‑69 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                                                                 <  1:1044657/70‑1 (MQ=255)
     gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                                                <  1:1268447/68‑1 (MQ=255)
     gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                                                <  1:3016120/68‑1 (MQ=255)
     gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGa                                                                <  1:1010090/68‑1 (MQ=255)
      ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg                                                            >  1:1857168/1‑71 (MQ=255)
       cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTgg                                                           <  1:3503223/71‑1 (MQ=255)
                gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                                                     >  1:3469037/1‑68 (MQ=255)
                 cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                                                 <  1:3604802/71‑1 (MQ=255)
                 cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAaagaa                                                   >  1:2566236/1‑69 (MQ=255)
                     aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             >  1:1667077/1‑71 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                                            >  1:2983340/1‑71 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:3428842/71‑1 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2382705/71‑1 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:1753496/71‑1 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:150903/71‑1 (MQ=255)
                         taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                         >  1:3188782/2‑69 (MQ=255)
                         taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                         >  1:1700810/2‑69 (MQ=255)
                         taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                                           <  1:122186/68‑1 (MQ=255)
                         cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                                         >  1:724233/1‑71 (MQ=255)
                           tGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                                        <  1:766422/70‑1 (MQ=255)
                              tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2645659/63‑1 (MQ=255)
                              tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:38885/62‑1 (MQ=255)
                                cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCTCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:3191507/60‑1 (MQ=255)
                                cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:3880871/60‑1 (MQ=255)
                                cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                                  >  1:1328731/1‑71 (MQ=255)
                                 tgtttgttTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                                                 >  1:3225604/1‑55 (MQ=255)
                                  gtttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                                >  1:2727909/1‑71 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                                               >  1:737161/1‑55 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2236217/58‑1 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc                                 <  1:3240845/69‑1 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg                               <  1:1378872/71‑1 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                           >  1:3492533/1‑57 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                           >  1:2850384/1‑57 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                             >  1:3356221/1‑71 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                             >  1:1070097/1‑71 (MQ=255)
                                       tttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt                            <  1:3695223/70‑1 (MQ=255)
                                         tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                              <  1:729648/50‑1 (MQ=255)
                                         tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                                       >  1:2277568/1‑57 (MQ=255)
                                         tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa                          <  1:2329850/70‑1 (MQ=255)
                                          cTACGCGCCGTGACGGTCGCTCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:1979833/1‑71 (MQ=255)
                                          cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:1375815/1‑71 (MQ=255)
                                          cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:2331255/1‑71 (MQ=255)
                                          cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                         <  1:3189982/70‑1 (MQ=255)
                                            aCGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:851634/48‑1 (MQ=255)
                                             cgcgCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                      <  1:548553/70‑1 (MQ=255)
                                             cgcgCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                      <  1:1671666/70‑1 (MQ=255)
                                                 ccGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg                 <  1:1977237/71‑1 (MQ=255)
                                                     gACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                                               >  1:2444047/1‑37 (MQ=255)
                                                     gACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                              <  1:1411758/38‑1 (MQ=255)
                                                      aCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGcc            >  1:383128/1‑71 (MQ=255)
                                                          tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATgggg                                          >  1:3600125/1‑37 (MQ=255)
                                                          tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt        >  1:1313037/1‑71 (MQ=255)
                                                          tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt        <  1:2502337/71‑1 (MQ=255)
                                                            gctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt      <  1:1056735/71‑1 (MQ=255)
                                                            gctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt      <  1:1779906/71‑1 (MQ=255)
                                                             ctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        <  1:1563325/52‑1 (MQ=255)
                                                                 gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                         >  1:490806/1‑48 (MQ=255)
                                                                 gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGTTGGCCTGCTTTATGa  >  1:2309060/1‑71 (MQ=255)
                                                                    |                                                                   
TTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGA  >  minE/385164‑385298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: