Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,238 C→A 8.4% L150M (CTG→ATG)  cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2380CA 8.4% 185.0 / 2.9 59L150M (CTG→ATG) cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base C (23/31);  new base A (3/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAT  >  minE/385170‑385308
                                                                     |                                                                      
ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg                                                                      >  1:1857168/1‑71 (MQ=255)
 cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTgg                                                                     <  1:3503223/71‑1 (MQ=255)
          gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                                                               >  1:3469037/1‑68 (MQ=255)
           cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                                                           <  1:3604802/71‑1 (MQ=255)
           cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAaagaa                                                             >  1:2566236/1‑69 (MQ=255)
               aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       >  1:1667077/1‑71 (MQ=255)
                tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                                                      >  1:2983340/1‑71 (MQ=255)
                tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:3428842/71‑1 (MQ=255)
                tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:2382705/71‑1 (MQ=255)
                tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:150903/71‑1 (MQ=255)
                tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:1753496/71‑1 (MQ=255)
                   taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                                   >  1:3188782/2‑69 (MQ=255)
                   taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                                   >  1:1700810/2‑69 (MQ=255)
                   taTGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                                                     <  1:122186/68‑1 (MQ=255)
                   cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                                                   >  1:724233/1‑71 (MQ=255)
                     tGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                                                  <  1:766422/70‑1 (MQ=255)
                        tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:2645659/63‑1 (MQ=255)
                        tGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:38885/62‑1 (MQ=255)
                          cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCTCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:3191507/60‑1 (MQ=255)
                          cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:3880871/60‑1 (MQ=255)
                          cTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                                            >  1:1328731/1‑71 (MQ=255)
                           tgtttgttTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAagaaga                                                           >  1:3225604/1‑55 (MQ=255)
                            gtttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                                          >  1:2727909/1‑71 (MQ=255)
                             tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                                                         >  1:737161/1‑55 (MQ=255)
                             tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:2236217/58‑1 (MQ=255)
                             tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc                                           <  1:3240845/69‑1 (MQ=255)
                             tttgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg                                         <  1:1378872/71‑1 (MQ=255)
                               tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                                     >  1:3492533/1‑57 (MQ=255)
                               tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                                     >  1:2850384/1‑57 (MQ=255)
                               tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                                       >  1:3356221/1‑71 (MQ=255)
                               tgtttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                                       >  1:1070097/1‑71 (MQ=255)
                                 tttCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt                                      <  1:3695223/70‑1 (MQ=255)
                                   tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                                        <  1:729648/50‑1 (MQ=255)
                                   tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                                                 >  1:2277568/1‑57 (MQ=255)
                                   tCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa                                    <  1:2329850/70‑1 (MQ=255)
                                    cTACGCGCCGTGACGGTCGCTCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  >  1:1979833/1‑71 (MQ=255)
                                    cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  >  1:1375815/1‑71 (MQ=255)
                                    cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  >  1:2331255/1‑71 (MQ=255)
                                    cTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                                   <  1:3189982/70‑1 (MQ=255)
                                      aCGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:851634/48‑1 (MQ=255)
                                       cgcgCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                                <  1:1671666/70‑1 (MQ=255)
                                       cgcgCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                                <  1:548553/70‑1 (MQ=255)
                                           ccGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg                           <  1:1977237/71‑1 (MQ=255)
                                               gACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAgaga                                                         >  1:2444047/1‑37 (MQ=255)
                                               gACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                                        <  1:1411758/38‑1 (MQ=255)
                                                aCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGcc                      >  1:383128/1‑71 (MQ=255)
                                                    tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATgggg                                                    >  1:3600125/1‑37 (MQ=255)
                                                    tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt                  >  1:1313037/1‑71 (MQ=255)
                                                    tcgctcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt                  <  1:2502337/71‑1 (MQ=255)
                                                      gctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt                <  1:1056735/71‑1 (MQ=255)
                                                      gctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt                <  1:1779906/71‑1 (MQ=255)
                                                       ctcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  <  1:1563325/52‑1 (MQ=255)
                                                           gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                                   >  1:490806/1‑48 (MQ=255)
                                                           gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGTTGGCCTGCTTTATGa            >  1:2309060/1‑71 (MQ=255)
                                                               ggggAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCa         <  1:1366602/67‑1 (MQ=255)
                                                                gTGAGCTGGTCAAAGAAGAGATGGGGCCAACTGCCGGGGTGATTGCGCtggtg                         <  1:3630862/53‑1 (MQ=255)
                                                                   aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATg             <  1:801091/62‑1 (MQ=255)
                                                                   aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGt     <  1:1056047/70‑1 (MQ=255)
                                                                   aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTc    <  1:588010/71‑1 (MQ=255)
                                                                     cTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAt  >  1:2002480/1‑71 (MQ=255)
                                                                     |                                                                      
CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAT  >  minE/385170‑385308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: