Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,241 G→A 8.6% V151I (GTC→ATC)  cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2410GA 8.6% 168.1 / 5.3 58V151I (GTC→ATC) cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (23/30);  new base A (3/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

CGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAT  >  minE/385171‑385308
                                                                       |                                                                   
cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTgg                                                                     <  1:3503223/71‑1 (MQ=255)
         gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag                                                               >  1:3469037/1‑68 (MQ=255)
          cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                                           <  1:3604802/71‑1 (MQ=255)
          cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAaagaa                                                             >  1:2566236/1‑69 (MQ=255)
              aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       >  1:1667077/1‑71 (MQ=255)
               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                                                      >  1:2983340/1‑71 (MQ=255)
               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:3428842/71‑1 (MQ=255)
               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:2382705/71‑1 (MQ=255)
               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:150903/71‑1 (MQ=255)
               tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:1753496/71‑1 (MQ=255)
                  taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                                   >  1:3188782/2‑69 (MQ=255)
                  taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                                   >  1:1700810/2‑69 (MQ=255)
                  taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                                                     <  1:122186/68‑1 (MQ=255)
                  cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                                                   >  1:724233/1‑71 (MQ=255)
                    tGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                                                  <  1:766422/70‑1 (MQ=255)
                       tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:2645659/63‑1 (MQ=255)
                       tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:38885/62‑1 (MQ=255)
                         cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCTCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:3191507/60‑1 (MQ=255)
                         cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:3880871/60‑1 (MQ=255)
                         cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                                            >  1:1328731/1‑71 (MQ=255)
                          tgtttgttTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                                           >  1:3225604/1‑55 (MQ=255)
                           gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                                          >  1:2727909/1‑71 (MQ=255)
                            tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                                         >  1:737161/1‑55 (MQ=255)
                            tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                                      <  1:2236217/58‑1 (MQ=255)
                            tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc                                           <  1:3240845/69‑1 (MQ=255)
                            tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg                                         <  1:1378872/71‑1 (MQ=255)
                              tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                                     >  1:3492533/1‑57 (MQ=255)
                              tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                                     >  1:2850384/1‑57 (MQ=255)
                              tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                                       >  1:3356221/1‑71 (MQ=255)
                              tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                                       >  1:1070097/1‑71 (MQ=255)
                                tttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt                                      <  1:3695223/70‑1 (MQ=255)
                                  tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                                        <  1:729648/50‑1 (MQ=255)
                                  tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                                                 >  1:2277568/1‑57 (MQ=255)
                                  tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa                                    <  1:2329850/70‑1 (MQ=255)
                                   cTACGCGCCGTGACGGTCGC‑TCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  >  1:1979833/1‑71 (MQ=255)
                                   cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  >  1:2331255/1‑71 (MQ=255)
                                   cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  >  1:1375815/1‑71 (MQ=255)
                                   cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                                   <  1:3189982/70‑1 (MQ=255)
                                     aCGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                                       <  1:851634/48‑1 (MQ=255)
                                      cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                                <  1:548553/70‑1 (MQ=255)
                                      cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                                <  1:1671666/70‑1 (MQ=255)
                                          ccGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg                           <  1:1977237/71‑1 (MQ=255)
                                              gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                                         >  1:2444047/1‑37 (MQ=255)
                                              gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                                        <  1:1411758/38‑1 (MQ=255)
                                               aCGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGcc                      >  1:383128/1‑71 (MQ=255)
                                                   tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATgggg                                                    >  1:3600125/1‑37 (MQ=255)
                                                   tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt                  >  1:1313037/1‑71 (MQ=255)
                                                   tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt                  <  1:2502337/71‑1 (MQ=255)
                                                     gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt                <  1:1056735/71‑1 (MQ=255)
                                                     gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt                <  1:1779906/71‑1 (MQ=255)
                                                      c‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                                  <  1:1563325/52‑1 (MQ=255)
                                                          gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                                   >  1:490806/1‑48 (MQ=255)
                                                          gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGTTGGCCTGCTTTATGa            >  1:2309060/1‑71 (MQ=255)
                                                              ggggAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCa         <  1:1366602/67‑1 (MQ=255)
                                                               gTGAGCTGGTCAAAGAAGAGATGGGGCCAACTGCCGGGGTGATTGCGCtggtg                         <  1:3630862/53‑1 (MQ=255)
                                                                  aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATg             <  1:801091/62‑1 (MQ=255)
                                                                  aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGt     <  1:1056047/70‑1 (MQ=255)
                                                                  aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTc    <  1:588010/71‑1 (MQ=255)
                                                                    cTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAt  >  1:2002480/1‑71 (MQ=255)
                                                                      ggTCAAAGAAGAGATGGGGCTAACCGCCGGGGTGATTgc                                <  1:2102934/39‑1 (MQ=255)
                                                                       |                                                                   
CGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAT  >  minE/385171‑385308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑