| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,184 | G→T | 11.6% | V227V (GTG→GTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,184 | 0 | G | T | 11.6% | 239.5 / 9.4 | 76 | V227V (GTG→GTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (32/35); new base T (2/7); total (34/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCGCGGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCA > minE/189114‑189252 | tCGCGCGGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATtgtg < 1:1837532/71‑1 (MQ=255) cgcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg > 1:3800370/1‑71 (MQ=255) cgcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg > 1:3379104/1‑71 (MQ=255) cgcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg > 1:2504122/1‑71 (MQ=255) cgcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg > 1:1808578/1‑71 (MQ=255) gcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTggg > 1:2470260/1‑71 (MQ=255) gcgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg < 1:2648124/70‑1 (MQ=255) cgcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGt < 1:414653/71‑1 (MQ=255) gcgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGt < 1:3888113/70‑1 (MQ=255) cgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTAt < 1:3473156/71‑1 (MQ=255) cgGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTAt < 1:1016025/71‑1 (MQ=255) ggACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTAt < 1:1148431/70‑1 (MQ=255) ggACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:3318694/71‑1 (MQ=255) gACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg > 1:2993479/1‑70 (MQ=255) gACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg > 1:3458608/1‑70 (MQ=255) aCTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTa < 1:2389298/71‑1 (MQ=255) ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgcc > 1:2834480/1‑71 (MQ=255) ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgcc > 1:877985/1‑71 (MQ=255) ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgc > 1:2669286/1‑70 (MQ=255) ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgc > 1:400174/1‑70 (MQ=255) ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgc < 1:2147232/70‑1 (MQ=255) ggCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgccg < 1:941026/70‑1 (MQ=255) gcgcGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCcgcg > 1:1330996/1‑71 (MQ=255) gcgGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATtgtg < 1:1769666/54‑1 (MQ=255) cgGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCa < 1:775655/71‑1 (MQ=255) ggAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCAt < 1:3144273/71‑1 (MQ=255) gAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATc > 1:2717643/1‑71 (MQ=255) aaCAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATg < 1:1880775/56‑1 (MQ=255) aaCAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgc < 1:2429123/61‑1 (MQ=255) aCAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCg < 1:3200051/71‑1 (MQ=255) cAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCgg > 1:3109812/1‑71 (MQ=255) cAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCgg < 1:951995/71‑1 (MQ=255) agTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg > 1:2208361/1‑71 (MQ=255) agTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg > 1:3779182/1‑71 (MQ=255) agTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg < 1:2002719/71‑1 (MQ=255) gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGt < 1:3892865/71‑1 (MQ=255) atatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGcc < 1:3010056/58‑1 (MQ=255) tatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGc < 1:1843947/71‑1 (MQ=255) tCGTTACCAACGGAACATCGACGTCGAACAAAATTGTgg < 1:2508023/39‑1 (MQ=255) tCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg > 1:2600727/1‑71 (MQ=255) tCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg > 1:1964065/1‑71 (MQ=255) ttACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:1473330/71‑1 (MQ=255) ttACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:3141094/71‑1 (MQ=255) tgaCCAACGGTACTTCCACTGCGAACAAAATTGTTGGTATGTACTCTGCTCCAgcaggcag < 1:542284/59‑1 (MQ=255) tACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGt < 1:2689639/41‑1 (MQ=255) aCCAACGGAACATCGACGTCGAACAAAATTGTggg > 1:3894720/1‑35 (MQ=255) aCCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGAt > 1:690367/1‑71 (MQ=255) ccAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGa < 1:235535/69‑1 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:2743344/71‑1 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:3005263/71‑1 (MQ=255) aCGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc > 1:3720346/1‑68 (MQ=255) aCGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc > 1:820214/1‑68 (MQ=255) ggAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg < 1:1628883/60‑1 (MQ=255) cATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:3336808/1‑48 (MQ=255) cATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:642726/1‑48 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:930947/1‑71 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:233731/1‑71 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:6910/1‑71 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:1803808/55‑1 (MQ=255) aCGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCa > 1:1704588/1‑71 (MQ=255) ctgcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaa < 1:248430/61‑1 (MQ=255) cGTCGAACAAAATCGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGa > 1:1360648/1‑58 (MQ=255) tgcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCAc < 1:2661353/43‑1 (MQ=255) tgcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCAc < 1:2711827/43‑1 (MQ=255) gTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa < 1:3052458/63‑1 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGc < 1:3079407/46‑1 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaa < 1:3226810/71‑1 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaa > 1:3658149/2‑62 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa > 1:1595306/1‑71 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaa < 1:958532/70‑1 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaa < 1:1529292/70‑1 (MQ=255) gAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACAAAt < 1:1642960/71‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:3643118/1‑38 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaaa < 1:3724611/70‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:3053044/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc > 1:632539/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3667322/71‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3354540/71‑1 (MQ=255) aCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCg > 1:1985098/1‑71 (MQ=255) aCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCg > 1:2615817/1‑71 (MQ=255) cAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGc > 1:1673698/1‑71 (MQ=255) aaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTg > 1:3691979/1‑71 (MQ=255) aaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGc < 1:1870698/38‑1 (MQ=255) aaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGAt > 1:780675/1‑45 (MQ=255) aaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGAt > 1:1246270/1‑45 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAATGTCATAAATCGCTgg < 1:845810/70‑1 (MQ=255) tgtgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGgcg > 1:1209976/1‑70 (MQ=255) gtgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCa > 1:146827/1‑71 (MQ=255) | TCGCGCGGACTTTTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCA > minE/189114‑189252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |