Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,418 C→A 14.9% L210M (CTG→ATG)  cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,4180CA14.9% 171.8 / 8.9 59L210M (CTG→ATG) cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base C (23/27);  new base A (6/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCC  >  minE/385353‑385486
                                                                 |                                                                    
aTAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCtcc                                                                   >  1:2972575/1‑67 (MQ=255)
aTAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTgc                                                                   >  1:1020216/1‑69 (MQ=255)
          gggAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTc                                                       <  1:1058188/71‑1 (MQ=255)
             aaCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCgg                                                    >  1:92002/1‑71 (MQ=255)
             aaCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCgg                                                    <  1:2936589/71‑1 (MQ=255)
              aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCggg                                                   <  1:3534153/71‑1 (MQ=255)
              aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCggg                                                   >  1:3784579/1‑71 (MQ=255)
                atatACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGc                                                 >  1:1397155/1‑71 (MQ=255)
                   taCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGt                                               <  1:1355792/70‑1 (MQ=255)
                   taCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGt                                               <  1:2984431/70‑1 (MQ=255)
                   taCCGTTGCGTTCACCATTCCGCTCGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGt                                               <  1:3746699/70‑1 (MQ=255)
                    aCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGTGATCTACCTGCGCTATCTGCGTCCGGGGCGTAt                                             >  1:1814932/1‑71 (MQ=255)
                     ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTAt                                                              >  1:2354023/1‑53 (MQ=255)
                     ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt                                            >  1:3842965/1‑71 (MQ=255)
                          gCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg                                        <  1:618196/70‑1 (MQ=255)
                           cGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTa                                                               <  1:128096/46‑1 (MQ=255)
                             ttCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATc                                                             >  1:1176646/1‑46 (MQ=255)
                               cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg                                        >  1:66222/1‑65 (MQ=255)
                                aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                  <  1:1025092/70‑1 (MQ=255)
                                aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                  <  1:2085597/70‑1 (MQ=255)
                                aCCATTCCGCTGGCGCTGTTTAGGGGGATCTACCTGCGCTATCTGCGTcc                                                      <  1:2694765/50‑1 (MQ=255)
                                 ccATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg                                <  1:3562301/71‑1 (MQ=255)
                                  cATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt                                                                <  1:3481994/38‑1 (MQ=255)
                                  cATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt                                                                <  1:3478850/38‑1 (MQ=255)
                                  cATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCgg                               <  1:1839672/71‑1 (MQ=255)
                                    ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGt                              >  1:1935080/1‑70 (MQ=255)
                                     tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa                            >  1:1795004/1‑71 (MQ=255)
                                       cGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc                          <  1:2284494/71‑1 (MQ=255)
                                       cGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc                          <  1:2778612/71‑1 (MQ=255)
                                         attgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTgg                        >  1:3567367/4‑71 (MQ=255)
                                         attgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTgg                        >  1:3002723/4‑71 (MQ=255)
                                          ttgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCa                            <  1:188346/64‑1 (MQ=255)
                                          tGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCgg                        >  1:957080/1‑70 (MQ=255)
                                            gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCAtt                          >  1:1990209/1‑65 (MQ=255)
                                            gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc                      >  1:3244522/1‑70 (MQ=255)
                                            gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc                      >  1:2757177/1‑70 (MQ=255)
                                            gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGAGTATTGGCGAAGTGTCGGTCATCGGTc                      >  1:1934121/1‑70 (MQ=255)
                                            gcgcTGTTTATGGGGATCTACCTGCACTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGt                       <  1:3056243/69‑1 (MQ=255)
                                             cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc                    >  1:75250/1‑68 (MQ=255)
                                             cgcTGTTTATGGGGGTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                    >  1:2216504/1‑71 (MQ=255)
                                             cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTg                                           >  1:195986/1‑48 (MQ=255)
                                             cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                    <  1:1875192/71‑1 (MQ=255)
                                             cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                    <  1:37189/71‑1 (MQ=255)
                                             cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                    <  1:467657/71‑1 (MQ=255)
                                              gcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGtctc                                 <  1:2799748/57‑1 (MQ=255)
                                              gcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCAttg                         >  1:2751892/1‑63 (MQ=255)
                                                  tttATGGGTATCTTCATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgt                                    >  1:707868/1‑50 (MQ=255)
                                                  tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                           <  1:2042298/59‑1 (MQ=255)
                                                  tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                           <  1:2046690/59‑1 (MQ=255)
                                                  tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                           <  1:3002841/59‑1 (MQ=255)
                                                  tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt                >  1:3533357/1‑70 (MQ=255)
                                                   ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                 >  1:1104422/1‑52 (MQ=255)
                                                    tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                     >  1:3270449/1‑47 (MQ=255)
                                                     aTGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCt            >  1:3800526/1‑71 (MQ=255)
                                                      tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATTGGTCTGGTATTCCt            >  1:3697298/1‑70 (MQ=255)
                                                         ggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt        <  1:335181/71‑1 (MQ=255)
                                                          gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt       <  1:331608/71‑1 (MQ=255)
                                                             cTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgt                                    <  1:2034263/39‑1 (MQ=255)
                                                              tACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                 <  1:417097/41‑1 (MQ=255)
                                                                ccTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGcc  <  1:3685279/70‑1 (MQ=255)
                                                                ccTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAGTCCTGATTTTc     <  1:1563374/67‑1 (MQ=255)
                                                                 |                                                                    
ATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCC  >  minE/385353‑385486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: