| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,442 | A→G | 16.7% | I218V (ATT→GTT) ‡ | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,442 | 0 | A | G | 16.7% | 120.3 / 17.2 | 54 | I218V (ATT→GTT) ‡ | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base A (21/24); new base G (6/3); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
ACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGG > minE/385373‑385508 | aCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGTGATCTACCTGCGCTATCTGCGTCCGGGGCGTAt > 1:1814932/1‑71 (MQ=255) ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt > 1:3842965/1‑71 (MQ=255) gCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg < 1:618196/70‑1 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg > 1:66222/1‑65 (MQ=255) aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt < 1:1025092/70‑1 (MQ=255) aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt < 1:2085597/70‑1 (MQ=255) ccATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg < 1:3562301/71‑1 (MQ=255) cATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCgg < 1:1839672/71‑1 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGt > 1:1935080/1‑70 (MQ=255) tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa > 1:1795004/1‑71 (MQ=255) cGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc < 1:2778612/71‑1 (MQ=255) cGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc < 1:2284494/71‑1 (MQ=255) attgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTgg > 1:3002723/4‑71 (MQ=255) attgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTgg > 1:3567367/4‑71 (MQ=255) ttgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCa < 1:188346/64‑1 (MQ=255) tGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCgg > 1:957080/1‑70 (MQ=255) gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCAtt > 1:1990209/1‑65 (MQ=255) gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc > 1:3244522/1‑70 (MQ=255) gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc > 1:2757177/1‑70 (MQ=255) gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGAGTATTGGCGAAGTGTCGGTCATCGGTc > 1:1934121/1‑70 (MQ=255) gcgcTGTTTATGGGGATCTACCTGCACTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGt < 1:3056243/69‑1 (MQ=255) cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc > 1:75250/1‑68 (MQ=255) cgcTGTTTATGGGGGTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg > 1:2216504/1‑71 (MQ=255) cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTg > 1:195986/1‑48 (MQ=255) cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg < 1:37189/71‑1 (MQ=255) cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg < 1:1875192/71‑1 (MQ=255) cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg < 1:467657/71‑1 (MQ=255) gcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGtctc < 1:2799748/57‑1 (MQ=255) gcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCAttg > 1:2751892/1‑63 (MQ=255) tttATGGGTATCTTCATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgt > 1:707868/1‑50 (MQ=255) tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt < 1:3002841/59‑1 (MQ=255) tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt < 1:2046690/59‑1 (MQ=255) tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt < 1:2042298/59‑1 (MQ=255) tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt > 1:3533357/1‑70 (MQ=255) ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:1104422/1‑52 (MQ=255) tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg > 1:3270449/1‑47 (MQ=255) aTGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCt > 1:3800526/1‑71 (MQ=255) tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATTGGTCTGGTATTCCt > 1:3697298/1‑70 (MQ=255) ggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt < 1:335181/71‑1 (MQ=255) gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt < 1:331608/71‑1 (MQ=255) cTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgt < 1:2034263/39‑1 (MQ=255) tACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc < 1:417097/41‑1 (MQ=255) ccTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGcc < 1:3685279/70‑1 (MQ=255) ccTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAGTCCTGATTTTc < 1:1563374/67‑1 (MQ=255) gcgcTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg > 1:1822822/1‑50 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTc > 1:106973/1‑49 (MQ=255) gTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc > 1:3499293/1‑71 (MQ=255) tCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCt > 1:1721202/1‑71 (MQ=255) cGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCt < 1:2872152/69‑1 (MQ=255) cGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTgg < 1:2481681/71‑1 (MQ=255) ggCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTg > 1:1875291/1‑71 (MQ=255) ggCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTg < 1:3066226/71‑1 (MQ=255) gCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTgg < 1:1817727/71‑1 (MQ=255) cGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTa < 1:3765057/52‑1 (MQ=255) | ACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGG > minE/385373‑385508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |