Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,444 T→G 17.2% I218M (ATT→ATG) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,4440TG17.2% 93.4 / 19.6 52I218M (ATT→ATG) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base G (6/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGA  >  minE/385374‑385512
                                                                      |                                                                    
ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt                                                                      >  1:3842965/1‑71 (MQ=255)
     gCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg                                                                  <  1:618196/70‑1 (MQ=255)
          cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg                                                                  >  1:66222/1‑65 (MQ=255)
           aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                                            <  1:2085597/70‑1 (MQ=255)
           aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                                            <  1:1025092/70‑1 (MQ=255)
            ccATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg                                                          <  1:3562301/71‑1 (MQ=255)
             cATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCgg                                                         <  1:1839672/71‑1 (MQ=255)
               ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGt                                                        >  1:1935080/1‑70 (MQ=255)
                tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa                                                      >  1:1795004/1‑71 (MQ=255)
                  cGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc                                                    <  1:2284494/71‑1 (MQ=255)
                  cGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc                                                    <  1:2778612/71‑1 (MQ=255)
                    attgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTgg                                                  >  1:3567367/4‑71 (MQ=255)
                    attgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTgg                                                  >  1:3002723/4‑71 (MQ=255)
                     ttgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCa                                                      <  1:188346/64‑1 (MQ=255)
                     tGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCgg                                                  >  1:957080/1‑70 (MQ=255)
                       gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCAtt                                                    >  1:1990209/1‑65 (MQ=255)
                       gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc                                                >  1:2757177/1‑70 (MQ=255)
                       gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc                                                >  1:3244522/1‑70 (MQ=255)
                       gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGAGTATTGGCGAAGTGTCGGTCATCGGTc                                                >  1:1934121/1‑70 (MQ=255)
                       gcgcTGTTTATGGGGATCTACCTGCACTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGt                                                 <  1:3056243/69‑1 (MQ=255)
                        cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc                                              >  1:75250/1‑68 (MQ=255)
                        cgcTGTTTATGGGGGTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                                              >  1:2216504/1‑71 (MQ=255)
                        cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTg                                                                     >  1:195986/1‑48 (MQ=255)
                        cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                                              <  1:1875192/71‑1 (MQ=255)
                        cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                                              <  1:37189/71‑1 (MQ=255)
                        cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTg                                              <  1:467657/71‑1 (MQ=255)
                         gcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGtctc                                                           <  1:2799748/57‑1 (MQ=255)
                         gcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCAttg                                                   >  1:2751892/1‑63 (MQ=255)
                             tttATGGGTATCTTCATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgt                                                              >  1:707868/1‑50 (MQ=255)
                             tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                                                     <  1:3002841/59‑1 (MQ=255)
                             tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                                                     <  1:2046690/59‑1 (MQ=255)
                             tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                                                     <  1:2042298/59‑1 (MQ=255)
                             tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt                                          >  1:3533357/1‑70 (MQ=255)
                              ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                                           >  1:1104422/1‑52 (MQ=255)
                               tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                                               >  1:3270449/1‑47 (MQ=255)
                                aTGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCt                                      >  1:3800526/1‑71 (MQ=255)
                                 tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATTGGTCTGGTATTCCt                                      >  1:3697298/1‑70 (MQ=255)
                                    ggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt                                  <  1:335181/71‑1 (MQ=255)
                                     gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt                                 <  1:331608/71‑1 (MQ=255)
                                        cTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgt                                                              <  1:2034263/39‑1 (MQ=255)
                                         tACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                                           <  1:417097/41‑1 (MQ=255)
                                           ccTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGcc                            <  1:3685279/70‑1 (MQ=255)
                                           ccTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAGTCCTGATTTTc                               <  1:1563374/67‑1 (MQ=255)
                                              gcgcTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg                                             >  1:1822822/1‑50 (MQ=255)
                                                    tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTc                                        >  1:106973/1‑49 (MQ=255)
                                                         gTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc             >  1:3499293/1‑71 (MQ=255)
                                                          tCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCt            >  1:1721202/1‑71 (MQ=255)
                                                            cGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCt            <  1:2872152/69‑1 (MQ=255)
                                                            cGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTgg          <  1:2481681/71‑1 (MQ=255)
                                                               ggCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTg       >  1:1875291/1‑71 (MQ=255)
                                                               ggCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTg       <  1:3066226/71‑1 (MQ=255)
                                                                gCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTgg      <  1:1817727/71‑1 (MQ=255)
                                                                 cGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTa                        <  1:3765057/52‑1 (MQ=255)
                                                                     ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCt            <  1:1414505/60‑1 (MQ=255)
                                                                     ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGa  >  1:647727/1‑70 (MQ=255)
                                                                     ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGa  >  1:2065247/1‑70 (MQ=255)
                                                                      tGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct                   <  1:407579/52‑1 (MQ=255)
                                                                      |                                                                    
CCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGA  >  minE/385374‑385512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: