| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,816 | T→C | 20.3% | H342H (CAT→CAC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,816 | 0 | T | C | 20.3% | 116.8 / 25.9 | 59 | H342H (CAT→CAC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (27/20); new base C (3/9); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
GGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGC > minE/385747‑385882 | ggTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCAGCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg > 1:3508245/1‑71 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCt < 1:1213685/71‑1 (MQ=255) ggACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCt > 1:2096827/1‑70 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt < 1:551934/71‑1 (MQ=255) ccGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCAt > 1:3712824/1‑62 (MQ=255) tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt < 1:2811760/71‑1 (MQ=255) aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:1538696/1‑71 (MQ=255) aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:589601/1‑71 (MQ=255) aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:1879872/1‑71 (MQ=255) ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt < 1:3291672/59‑1 (MQ=255) ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCggta < 1:516376/70‑3 (MQ=255) tCCCGTTCATGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc > 1:2241936/1‑70 (MQ=255) ccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:198973/70‑3 (MQ=255) ccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:2624561/70‑3 (MQ=255) cGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc > 1:3610705/1‑70 (MQ=255) cGTTCCTGTTGATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTacgacg > 1:3111843/1‑71 (MQ=255) cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAGGCCGaa < 1:513094/71‑1 (MQ=255) gTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc > 1:2085774/1‑51 (MQ=255) gTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:871837/1‑67 (MQ=255) gTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:345865/62‑3 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgca > 1:2946110/1‑41 (MQ=255) aTCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCt < 1:2748866/71‑1 (MQ=255) aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:3476404/70‑1 (MQ=255) aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCt > 1:793095/1‑71 (MQ=255) tCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTg < 1:1647770/71‑1 (MQ=255) tCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTg < 1:652499/71‑1 (MQ=255) aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGAt > 1:301806/1‑41 (MQ=255) ccATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:3153357/66‑1 (MQ=255) cATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGGTGATCTCTTCTGGGACCACGc > 1:973459/1‑56 (MQ=255) aTCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaa < 1:983120/71‑1 (MQ=255) aTCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaa < 1:609374/71‑1 (MQ=255) tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac > 1:3689463/1‑71 (MQ=255) gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga < 1:2135801/71‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:1849348/71‑1 (MQ=255) ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaa < 1:2030088/71‑1 (MQ=255) ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaa < 1:2278416/71‑1 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg < 1:1613947/71‑1 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg > 1:2297925/1‑71 (MQ=255) tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc > 1:3047714/1‑67 (MQ=255) tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaac < 1:3812158/69‑3 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGc < 1:277114/47‑1 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGc < 1:2970180/71‑1 (MQ=255) gcgcGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcga < 1:910831/60‑1 (MQ=255) gcgcGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAg < 1:3158300/71‑1 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGt > 1:2231017/1‑70 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:2903705/1‑71 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:2899356/1‑71 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:969233/1‑71 (MQ=255) cTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:3010349/1‑70 (MQ=255) tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg < 1:1400676/66‑1 (MQ=255) tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa > 1:611994/1‑71 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa < 1:3108896/69‑1 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc > 1:2382780/1‑71 (MQ=255) cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg > 1:3858316/1‑63 (MQ=255) cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCg > 1:805865/1‑71 (MQ=255) ttCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg > 1:1392380/1‑45 (MQ=255) ttCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCgg > 1:1162794/1‑71 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCTAACGAAGGGCAGGCGTGCTTTAt > 1:1428709/1‑66 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAgg < 1:230622/51‑1 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc > 1:819680/1‑70 (MQ=255) | GGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGC > minE/385747‑385882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |