| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,847 | A→C | 10.6% | M353L (ATG→CTG) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,847 | 0 | A | C | 10.6% | 147.5 / 14.5 | 66 | M353L (ATG→CTG) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base A (30/29); new base C (2/5); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
ATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGA > minE/385781‑385916 | aTCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCt < 1:2748866/71‑1 (MQ=255)aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:3476404/70‑1 (MQ=255)aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCt > 1:793095/1‑71 (MQ=255) tCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTg < 1:1647770/71‑1 (MQ=255) tCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTg < 1:652499/71‑1 (MQ=255) ccATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:3153357/66‑1 (MQ=255) aTCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaa < 1:983120/71‑1 (MQ=255) aTCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaa < 1:609374/71‑1 (MQ=255) tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac > 1:3689463/1‑71 (MQ=255) gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga < 1:2135801/71‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:1849348/71‑1 (MQ=255) ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaa < 1:2278416/71‑1 (MQ=255) ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaa < 1:2030088/71‑1 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg > 1:2297925/1‑71 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg < 1:1613947/71‑1 (MQ=255) tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc > 1:3047714/1‑67 (MQ=255) tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaac < 1:3812158/69‑3 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGc < 1:2970180/71‑1 (MQ=255) gcgcGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcga < 1:910831/60‑1 (MQ=255) gcgcGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAg < 1:3158300/71‑1 (MQ=255) gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGt > 1:2231017/1‑70 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:2899356/1‑71 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:2903705/1‑71 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:969233/1‑71 (MQ=255) cTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:3010349/1‑70 (MQ=255) tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg < 1:1400676/66‑1 (MQ=255) tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa > 1:611994/1‑71 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa < 1:3108896/69‑1 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc > 1:2382780/1‑71 (MQ=255) cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg > 1:3858316/1‑63 (MQ=255) cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCg > 1:805865/1‑71 (MQ=255) ttCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg > 1:1392380/1‑45 (MQ=255) ttCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCgg > 1:1162794/1‑71 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCTAACGAAGGGCAGGCGTGCTTTAt > 1:1428709/1‑66 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAgg < 1:230622/51‑1 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc > 1:819680/1‑70 (MQ=255) gcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTACgg > 1:3022534/1‑71 (MQ=255) gcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTg < 1:3509131/71‑1 (MQ=255) cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg < 1:1662811/71‑1 (MQ=255) cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg > 1:3016323/1‑71 (MQ=255) cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTg < 1:1583949/70‑1 (MQ=255) tGATCTCTTCTGGGACCACTCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt < 1:2659821/56‑1 (MQ=255) tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt < 1:1582497/56‑1 (MQ=255) tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg > 1:374929/1‑70 (MQ=255) gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:88656/39‑1 (MQ=255) gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:1591502/39‑1 (MQ=255) aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTggg < 1:3076262/69‑1 (MQ=255) tctcTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTg > 1:696242/1‑66 (MQ=255) ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa > 1:3139172/1‑71 (MQ=255) tctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt > 1:515839/1‑67 (MQ=255) ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGc > 1:3580757/1‑44 (MQ=255) ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATg > 1:2695806/1‑71 (MQ=255) gggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg > 1:1923530/1‑70 (MQ=255) ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg < 1:2062311/69‑1 (MQ=255) ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGa < 1:3520558/70‑1 (MQ=255) cACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCt < 1:3416980/71‑1 (MQ=255) aCGCCGAAGATGGTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa > 1:1521606/1‑55 (MQ=255) cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc > 1:424096/1‑71 (MQ=255) cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc > 1:2964384/1‑71 (MQ=255) ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa < 1:46536/52‑1 (MQ=255) ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg < 1:3240639/70‑1 (MQ=255) ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGt > 1:3810750/1‑71 (MQ=255) gAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg < 1:1053513/59‑1 (MQ=255) aGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGc > 1:961064/1‑70 (MQ=255) aGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCg < 1:1370659/71‑1 (MQ=255) gATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa > 1:2975520/1‑71 (MQ=255) aTGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt < 1:2360372/62‑1 (MQ=255) | ATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGA > minE/385781‑385916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |