Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,890 G→C 9.8% G367A (GGG→GCG) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,8900GC 9.8% 167.6 / 7.9 61G367A (GGG→GCG) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (21/34);  new base C (3/3);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCG  >  minE/385820‑385955
                                                                      |                                                                 
cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                   <  1:1662811/71‑1 (MQ=255)
cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                   >  1:3016323/1‑71 (MQ=255)
 tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                   >  1:374929/1‑70 (MQ=255)
   aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTggg                                                                  <  1:3076262/69‑1 (MQ=255)
        ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                           >  1:3139172/1‑71 (MQ=255)
         tctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                              >  1:515839/1‑67 (MQ=255)
          ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATg                                                         >  1:2695806/1‑71 (MQ=255)
            gggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                        >  1:1923530/1‑70 (MQ=255)
             ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                        <  1:2062311/69‑1 (MQ=255)
             ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGa                                                       <  1:3520558/70‑1 (MQ=255)
                 cACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCt                                                  <  1:3416980/71‑1 (MQ=255)
                  aCGCCGAAGATGGTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa                                                                 >  1:1521606/1‑55 (MQ=255)
                   cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                >  1:424096/1‑71 (MQ=255)
                   cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                >  1:2964384/1‑71 (MQ=255)
                     ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa                                                                 <  1:46536/52‑1 (MQ=255)
                     ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                               <  1:3240639/70‑1 (MQ=255)
                     ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGt                                              >  1:3810750/1‑71 (MQ=255)
                       gAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                        <  1:1053513/59‑1 (MQ=255)
                         aGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGc                                           >  1:961064/1‑70 (MQ=255)
                         aGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCg                                          <  1:1370659/71‑1 (MQ=255)
                          gATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                         >  1:2975520/1‑71 (MQ=255)
                           aTGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                 <  1:2360372/62‑1 (MQ=255)
                             gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                        <  1:1322370/53‑1 (MQ=255)
                              cTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                      <  1:2590601/70‑1 (MQ=255)
                                ggCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGAt                                                                <  1:1800736/42‑1 (MQ=255)
                                ggCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATgg                                   >  1:1670497/1‑71 (MQ=255)
                                 gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa                                                                 <  1:3298039/40‑1 (MQ=255)
                                  cgaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                            <  1:1533634/44‑1 (MQ=255)
                                   gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgcgc                                <  1:2362075/71‑1 (MQ=255)
                                   gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgcgc                                <  1:1644782/71‑1 (MQ=255)
                                     acgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTg                              >  1:3691207/1‑71 (MQ=255)
                                      cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAtt                                       <  1:193480/61‑1 (MQ=255)
                                      cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTgg                             >  1:3753475/1‑71 (MQ=255)
                                      cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTgg                             <  1:2101763/71‑1 (MQ=255)
                                      cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTgg                             <  1:3631336/71‑1 (MQ=255)
                                       gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                           <  1:3488949/40‑1 (MQ=255)
                                       gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                            <  1:74886/71‑1 (MQ=255)
                                        aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTAGTGTCGATTATGGCGCTGGtt                           <  1:803030/71‑1 (MQ=255)
                                         aGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGtt                           >  1:1577707/1‑70 (MQ=255)
                                           ggCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATgg                                   >  1:2966414/1‑60 (MQ=255)
                                            gCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                        <  1:1018107/38‑1 (MQ=255)
                                            gCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                        <  1:1839821/54‑1 (MQ=255)
                                            gCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                        <  1:3757929/70‑1 (MQ=255)
                                             cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                      <  1:872134/71‑1 (MQ=255)
                                             cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                      >  1:3611217/1‑71 (MQ=255)
                                             cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                      <  1:3642744/71‑1 (MQ=255)
                                                   tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                 <  1:1470265/38‑1 (MQ=255)
                                                   tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                <  1:475505/71‑1 (MQ=255)
                                                     cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc               >  1:1265994/1‑70 (MQ=255)
                                                      tttATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                        <  1:178905/44‑1 (MQ=255)
                                                       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc            >  1:1675089/3‑71 (MQ=255)
                                                        tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc            >  1:1590061/1‑70 (MQ=255)
                                                        tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc            >  1:2580016/1‑70 (MQ=255)
                                                        caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc            <  1:2777135/69‑1 (MQ=255)
                                                        caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc            >  1:1201926/2‑70 (MQ=255)
                                                         aTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGa          <  1:1146750/71‑1 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa          <  1:3352639/70‑1 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt         >  1:2968835/1‑71 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTACGCCTGTATCATCGAt         <  1:174871/71‑1 (MQ=255)
                                                           cGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt         <  1:1027440/70‑1 (MQ=255)
                                                            ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATc        <  1:2534966/70‑1 (MQ=255)
                                                            ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc       <  1:1272316/71‑1 (MQ=255)
                                                            ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGAGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac        >  1:3782389/1‑68 (MQ=255)
                                                              cTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg     >  1:1846836/1‑71 (MQ=255)
                                                                 cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCg  <  1:2697598/71‑1 (MQ=255)
                                                                      ggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg     <  1:2117396/63‑1 (MQ=255)
                                                                      |                                                                 
CTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCG  >  minE/385820‑385955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: