Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,897 A→G 9.8% L369L (TTA→TTG) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,8970AG 9.8% 159.8 / 8.5 61L369L (TTA→TTG) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (23/32);  new base G (3/3);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCC  >  minE/385828‑385966
                                                                     |                                                                     
ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                                      >  1:3139172/1‑71 (MQ=255)
  ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATg                                                                    >  1:2695806/1‑71 (MQ=255)
    gggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                                   >  1:1923530/1‑70 (MQ=255)
     ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                                   <  1:2062311/69‑1 (MQ=255)
     ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGa                                                                  <  1:3520558/70‑1 (MQ=255)
         cACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCt                                                             <  1:3416980/71‑1 (MQ=255)
           cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                           >  1:424096/1‑71 (MQ=255)
           cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                           >  1:2964384/1‑71 (MQ=255)
             ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                                          <  1:3240639/70‑1 (MQ=255)
             ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGt                                                         >  1:3810750/1‑71 (MQ=255)
               gAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                                   <  1:1053513/59‑1 (MQ=255)
                 aGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGc                                                      >  1:961064/1‑70 (MQ=255)
                 aGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCg                                                     <  1:1370659/71‑1 (MQ=255)
                  gATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                                    >  1:2975520/1‑71 (MQ=255)
                   aTGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                            <  1:2360372/62‑1 (MQ=255)
                     gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                                   <  1:1322370/53‑1 (MQ=255)
                      cTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                 <  1:2590601/70‑1 (MQ=255)
                        ggCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATgg                                              >  1:1670497/1‑71 (MQ=255)
                          cgaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                       <  1:1533634/44‑1 (MQ=255)
                           gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgcgc                                           <  1:1644782/71‑1 (MQ=255)
                           gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgcgc                                           <  1:2362075/71‑1 (MQ=255)
                             acgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTg                                         >  1:3691207/1‑71 (MQ=255)
                              cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAtt                                                  <  1:193480/61‑1 (MQ=255)
                              cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTgg                                        <  1:2101763/71‑1 (MQ=255)
                              cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTgg                                        >  1:3753475/1‑71 (MQ=255)
                              cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTgg                                        <  1:3631336/71‑1 (MQ=255)
                               gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                                      <  1:3488949/40‑1 (MQ=255)
                               gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                                       <  1:74886/71‑1 (MQ=255)
                                aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTAGTGTCGATTATGGCGCTGGtt                                      <  1:803030/71‑1 (MQ=255)
                                 aGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGtt                                      >  1:1577707/1‑70 (MQ=255)
                                   ggCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATgg                                              >  1:2966414/1‑60 (MQ=255)
                                    gCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                                   <  1:1018107/38‑1 (MQ=255)
                                    gCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                                   <  1:1839821/54‑1 (MQ=255)
                                    gCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                                   <  1:3757929/70‑1 (MQ=255)
                                     cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                 <  1:3642744/71‑1 (MQ=255)
                                     cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                 >  1:3611217/1‑71 (MQ=255)
                                     cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                 <  1:872134/71‑1 (MQ=255)
                                           tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                            <  1:1470265/38‑1 (MQ=255)
                                           tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                           <  1:475505/71‑1 (MQ=255)
                                             cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                          >  1:1265994/1‑70 (MQ=255)
                                              tttATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                                   <  1:178905/44‑1 (MQ=255)
                                               tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                       >  1:1675089/3‑71 (MQ=255)
                                                tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                       >  1:1590061/1‑70 (MQ=255)
                                                tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                       >  1:2580016/1‑70 (MQ=255)
                                                caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                       >  1:1201926/2‑70 (MQ=255)
                                                caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                       <  1:2777135/69‑1 (MQ=255)
                                                 aTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGa                     <  1:1146750/71‑1 (MQ=255)
                                                  tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                     <  1:3352639/70‑1 (MQ=255)
                                                  tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                    >  1:2968835/1‑71 (MQ=255)
                                                  tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTACGCCTGTATCATCGAt                    <  1:174871/71‑1 (MQ=255)
                                                   cGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                    <  1:1027440/70‑1 (MQ=255)
                                                    ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATc                   <  1:2534966/70‑1 (MQ=255)
                                                    ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                  <  1:1272316/71‑1 (MQ=255)
                                                    ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGAGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac                   >  1:3782389/1‑68 (MQ=255)
                                                      cTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg                >  1:1846836/1‑71 (MQ=255)
                                                         cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCg             <  1:2697598/71‑1 (MQ=255)
                                                              ggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg                <  1:2117396/63‑1 (MQ=255)
                                                                aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt      >  1:2733142/1‑71 (MQ=255)
                                                                 tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                 >  1:918385/1‑43 (MQ=255)
                                                                 tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                            >  1:324658/1‑48 (MQ=255)
                                                                 tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtttt     <  1:3409863/71‑1 (MQ=255)
                                                                 tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTCTCCGCCTGTATCATCGATcc                  >  1:743209/1‑58 (MQ=255)
                                                                  gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg               <  1:1559909/60‑1 (MQ=255)
                                                                  gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt      >  1:3677136/1‑69 (MQ=255)
                                                                     aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt      >  1:617591/1‑66 (MQ=255)
                                                                     aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt      <  1:1664047/66‑1 (MQ=255)
                                                                     aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGcc  >  1:406668/1‑70 (MQ=255)
                                                                     |                                                                     
TTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCC  >  minE/385828‑385966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: