Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,931 T→G 17.7% S381A (TCC→GCC) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,9310TG17.7% 116.2 / 31.2 68S381A (TCC→GCC) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (28/28);  new base G (7/5);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

GCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGG  >  minE/385864‑385997
                                                                   |                                                                  
gCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                                                                  <  1:3757929/70‑1 (MQ=255)
 cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                                                <  1:872134/71‑1 (MQ=255)
 cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                                                <  1:3642744/71‑1 (MQ=255)
 cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                                                >  1:3611217/1‑71 (MQ=255)
       tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                                                          <  1:475505/71‑1 (MQ=255)
         cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                                                         >  1:1265994/1‑70 (MQ=255)
           tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                      >  1:1675089/3‑71 (MQ=255)
            tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                                                      >  1:2580016/1‑70 (MQ=255)
            tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                                                      >  1:1590061/1‑70 (MQ=255)
            caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                      >  1:1201926/2‑70 (MQ=255)
            caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                      <  1:2777135/69‑1 (MQ=255)
             aTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGa                                                    <  1:1146750/71‑1 (MQ=255)
              tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                    <  1:3352639/70‑1 (MQ=255)
              tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                   >  1:2968835/1‑71 (MQ=255)
              tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTACGCCTGTATCATCGAt                                                   <  1:174871/71‑1 (MQ=255)
               cGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                   <  1:1027440/70‑1 (MQ=255)
                ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATc                                                  <  1:2534966/70‑1 (MQ=255)
                ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                                                 <  1:1272316/71‑1 (MQ=255)
                ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGAGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac                                                  >  1:3782389/1‑68 (MQ=255)
                  cTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg                                               >  1:1846836/1‑71 (MQ=255)
                     cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCg                                            <  1:2697598/71‑1 (MQ=255)
                          ggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg                                               <  1:2117396/63‑1 (MQ=255)
                            aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt                                     >  1:2733142/1‑71 (MQ=255)
                             tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                                                >  1:918385/1‑43 (MQ=255)
                             tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                                           >  1:324658/1‑48 (MQ=255)
                             tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtttt                                    <  1:3409863/71‑1 (MQ=255)
                             tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTCTCCGCCTGTATCATCGATcc                                                 >  1:743209/1‑58 (MQ=255)
                              gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg                                              <  1:1559909/60‑1 (MQ=255)
                              gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt                                     >  1:3677136/1‑69 (MQ=255)
                                 aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt                                     <  1:1664047/66‑1 (MQ=255)
                                 aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt                                     >  1:617591/1‑66 (MQ=255)
                                 aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGcc                                 >  1:406668/1‑70 (MQ=255)
                                  aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt                               >  1:2331529/1‑71 (MQ=255)
                                   tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc                                             >  1:2330920/1‑56 (MQ=255)
                                   tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                              <  1:3649642/71‑1 (MQ=255)
                                   tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                              <  1:1408146/71‑1 (MQ=255)
                                      aaTCCTTCGTGGCGATTATGGCGCTGGTTTCCGcc                                                               >  1:3150186/1‑35 (MQ=255)
                                      aaTCCTTCGTGGCGATTATGGCGCTGGTTTCCGcc                                                               >  1:3215326/1‑35 (MQ=255)
                                       gtCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACa                          <  1:2450192/70‑1 (MQ=255)
                                       aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                              >  1:1329927/1‑67 (MQ=255)
                                       aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa                             >  1:1494920/1‑68 (MQ=255)
                                       aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACa                          <  1:2018197/71‑1 (MQ=255)
                                        tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc                                             >  1:3828925/1‑51 (MQ=255)
                                        tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTg                                   <  1:2107254/61‑1 (MQ=255)
                                          cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc                       <  1:1425091/71‑1 (MQ=255)
                                          cTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACac                         >  1:2351686/1‑68 (MQ=255)
                                            tCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCgt                                           >  1:3333672/1‑49 (MQ=255)
                                            tCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc                      >  1:1677542/1‑70 (MQ=255)
                                             cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc                      <  1:3012109/69‑1 (MQ=255)
                                             cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc                    >  1:3528104/1‑70 (MQ=255)
                                              gTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtt                                      <  1:3694265/52‑1 (MQ=255)
                                              gTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                              <  1:3506746/60‑1 (MQ=255)
                                                     ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt             >  1:2256305/1‑70 (MQ=255)
                                                     ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt             >  1:3021694/1‑70 (MQ=255)
                                                     ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTg            <  1:14396/71‑1 (MQ=255)
                                                     ttATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGaa                            >  1:3722328/1‑55 (MQ=255)
                                                     ttATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGaa                            >  1:1355696/1‑55 (MQ=255)
                                                      tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAAc                           <  1:2112680/55‑1 (MQ=255)
                                                       aTGGCGCTGGTTTCCGCCTGTATCATCGATCCggg                                              >  1:808085/1‑35 (MQ=255)
                                                       aTGGCGCTGGTTTCCGCCTGTATCATCGATCCggg                                              >  1:3884738/1‑35 (MQ=255)
                                                       aTGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa                             <  1:1431758/52‑1 (MQ=255)
                                                        tGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt             <  1:2764697/67‑1 (MQ=255)
                                                           cgcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAGCAGCCCGATGGCGGTGCTGGCt      <  1:724819/71‑1 (MQ=255)
                                                           cgcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCt      <  1:449226/71‑1 (MQ=255)
                                                            gcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTc     >  1:1405036/1‑71 (MQ=255)
                                                             cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                              <  1:110534/45‑1 (MQ=255)
                                                             cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAAGAACAGcc                       >  1:2128327/1‑52 (MQ=255)
                                                                gTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCgg  <  1:3141410/70‑1 (MQ=255)
                                                                   tCCGCCTGTATCATCGATCCGGGCGTGTATTTTGcc                                 >  1:1259441/1‑36 (MQ=255)
                                                                   |                                                                  
GCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGG  >  minE/385864‑385997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: