| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,963 | T→C | 10.0% | F391F (TTT→TTC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,963 | 0 | T | C | 10.0% | 163.4 / 8.1 | 60 | F391F (TTT→TTC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (24/30); new base C (4/2); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGC > minE/385893‑386030 | tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtttt < 1:3409863/71‑1 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGcc > 1:406668/1‑70 (MQ=255) aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt > 1:2331529/1‑71 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3649642/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:1408146/71‑1 (MQ=255) gtCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACa < 1:2450192/70‑1 (MQ=255) aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg > 1:1329927/1‑67 (MQ=255) aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa > 1:1494920/1‑68 (MQ=255) aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACa < 1:2018197/71‑1 (MQ=255) tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTg < 1:2107254/61‑1 (MQ=255) cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc < 1:1425091/71‑1 (MQ=255) cTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACac > 1:2351686/1‑68 (MQ=255) tCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc > 1:1677542/1‑70 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:3012109/69‑1 (MQ=255) cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:3528104/1‑70 (MQ=255) gTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3506746/60‑1 (MQ=255) ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt > 1:3021694/1‑70 (MQ=255) ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt > 1:2256305/1‑70 (MQ=255) ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTg < 1:14396/71‑1 (MQ=255) ttATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGaa > 1:3722328/1‑55 (MQ=255) ttATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGaa > 1:1355696/1‑55 (MQ=255) tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAAc < 1:2112680/55‑1 (MQ=255) aTGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa < 1:1431758/52‑1 (MQ=255) tGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt < 1:2764697/67‑1 (MQ=255) cgcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAGCAGCCCGATGGCGGTGCTGGCt < 1:724819/71‑1 (MQ=255) cgcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCt < 1:449226/71‑1 (MQ=255) gcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTc > 1:1405036/1‑71 (MQ=255) cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:110534/45‑1 (MQ=255) cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAAGAACAGcc > 1:2128327/1‑52 (MQ=255) gTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCgg < 1:3141410/70‑1 (MQ=255) tCCGCCTGTATCATCGATCCGGGCGTGTATTTTGcc > 1:1259441/1‑36 (MQ=255) ccGCCTGTATCATCGATCCGGGCGTGTATTTTGcc > 1:375767/1‑35 (MQ=255) ccGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAgg > 1:2988646/1‑70 (MQ=255) cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAggg < 1:1597973/70‑1 (MQ=255) cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGa > 1:254284/1‑71 (MQ=255) cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGa > 1:3278000/1‑71 (MQ=255) ccTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcg < 1:285236/71‑1 (MQ=255) gTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcggcg > 1:3698815/1‑71 (MQ=255) tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGc < 1:2039867/54‑1 (MQ=255) tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGc < 1:1562283/54‑1 (MQ=255) tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcggcg < 1:2980094/70‑1 (MQ=255) catcGATCCGTGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGAtg > 1:2472677/1‑71 (MQ=255) atcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCgg > 1:2122624/1‑55 (MQ=255) cGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTgg > 1:3672266/1‑71 (MQ=255) cGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTgg > 1:1207560/1‑71 (MQ=255) gATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGc > 1:2911890/1‑53 (MQ=255) gATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCa > 1:547902/1‑54 (MQ=255) gATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTgg < 1:3684144/70‑1 (MQ=255) tCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGt < 1:3016322/69‑1 (MQ=255) cGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCt < 1:3695775/71‑1 (MQ=255) gggCGTGTATTTTGCCGTGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCtt < 1:310825/71‑1 (MQ=255) gggCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCtt < 1:635560/71‑1 (MQ=255) cGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGa < 1:793820/57‑1 (MQ=255) cGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTg < 1:3750849/71‑1 (MQ=255) cGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTg < 1:833616/71‑1 (MQ=255) gtgtATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTg < 1:3702515/70‑1 (MQ=255) tATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGa > 1:1773127/1‑53 (MQ=255) tATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcg > 1:2694424/1‑70 (MQ=255) tATTTTGCCATGAACAGACCGATGGCGTTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgc > 1:3099740/1‑71 (MQ=255) aTTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGt < 1:3126743/58‑1 (MQ=255) aTTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgc < 1:3277909/70‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg > 1:2200650/1‑70 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCgcgc < 1:2028226/71‑1 (MQ=255) tttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTTGTCGCTTATGCcgcg > 1:3863631/1‑69 (MQ=255) tttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg > 1:2864977/1‑69 (MQ=255) ttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGAtgtg > 1:2577348/1‑53 (MQ=255) | TGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGC > minE/385893‑386030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |