Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 386,420 A→G 8.2% I544V (ATT→GTT)  cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE386,4200AG 8.2% 170.2 / 5.6 61I544V (ATT→GTT) cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (22/34);  new base G (2/3);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCT  >  minE/386351‑386486
                                                                     |                                                                  
gCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCAt                                                                   <  1:22034/71‑1 (MQ=255)
gCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCAt                                                                   <  1:3093336/71‑1 (MQ=255)
 cAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCAtt                                                                  <  1:3042952/71‑1 (MQ=255)
  aaCAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTa                                                                 <  1:2616200/71‑1 (MQ=255)
   aCAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTaa                                                                <  1:1059878/71‑1 (MQ=255)
   aCAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTaa                                                                <  1:2394814/71‑1 (MQ=255)
   aCAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTaa                                                                <  1:2511833/71‑1 (MQ=255)
        gcTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACActc                                                           <  1:1992996/71‑1 (MQ=255)
            tgCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCtg                                                         <  1:1298947/69‑1 (MQ=255)
            tgCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCtg                                                         <  1:218731/69‑1 (MQ=255)
              cGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTgg                                                      <  1:2854286/70‑1 (MQ=255)
              cGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTgg                                                      <  1:3802474/70‑1 (MQ=255)
                tACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCg                                                   >  1:3546732/1‑71 (MQ=255)
                 aCTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGAGGCATTaa                                                                >  1:1781670/1‑57 (MQ=255)
                    ggCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGt                                               >  1:1556499/1‑71 (MQ=255)
                    ggCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGt                                               >  1:1572174/1‑71 (MQ=255)
                     gCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGGGGCATTAACACTCTGTGGCCGCTGtt                                              >  1:3860946/1‑71 (MQ=255)
                     gCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGtt                                              <  1:1008083/71‑1 (MQ=255)
                     gCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGtt                                              <  1:2578321/71‑1 (MQ=255)
                     gCGGGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCtg                                                         <  1:2022494/60‑1 (MQ=255)
                       gTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTg                                            >  1:3422852/1‑71 (MQ=255)
                        tGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTgg                                           <  1:1384757/71‑1 (MQ=255)
                          gggCTACTTCCTCCATCATGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGt                                          <  1:697887/70‑1 (MQ=255)
                          gggCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTa                                         >  1:633637/1‑71 (MQ=255)
                          gggCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCCGTGGCCGCTGtt                                              <  1:42016/66‑1 (MQ=255)
                           ggCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCtgtg                                                       >  1:90581/1‑56 (MQ=255)
                            gCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTAtt                                       <  1:1400524/71‑1 (MQ=255)
                              tacttactCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGtt                                              >  1:2705529/1‑62 (MQ=255)
                              tACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                     >  1:1419834/1‑71 (MQ=255)
                               aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCGGCTGTTTGGTATTGc                                     >  1:149171/1‑70 (MQ=255)
                               aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                     >  1:3195728/1‑70 (MQ=255)
                               aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                     >  1:141739/1‑70 (MQ=255)
                                cTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGcc                                    >  1:1986492/1‑70 (MQ=255)
                                 ttCCTCCATCAGGGCGTGGTCGATCCGCCGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCaa                                  >  1:2960936/1‑71 (MQ=255)
                                  tcctccATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCaa                                  >  1:2481852/1‑70 (MQ=255)
                                      ccATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAAc                                 >  1:2955634/1‑67 (MQ=255)
                                       cATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGAt                            <  1:155986/71‑1 (MQ=255)
                                        aTCAGGGCGTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTAtct                                      >  1:3356265/1‑58 (MQ=255)
                                        aTCAGGGCGTGGTCGATCCGCTTGGCGGCATTAACAct                                                            <  1:1214192/38‑1 (MQ=255)
                                        aTCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATg                           <  1:665327/71‑1 (MQ=255)
                                         tCAGGGCGTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATg                           <  1:1210251/70‑1 (MQ=255)
                                         tCAGGGCGTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATGc                          >  1:2737037/1‑71 (MQ=255)
                                         tCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAAc                                 >  1:2183047/1‑64 (MQ=255)
                                         tCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGc                          <  1:2140766/71‑1 (MQ=255)
                                             ggCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGc                      <  1:452322/71‑1 (MQ=255)
                                              gCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTgg                                           <  1:2442816/49‑1 (MQ=255)
                                              gCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTgg                                           <  1:3584432/49‑1 (MQ=255)
                                                gTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATGCt                         <  1:1683315/65‑1 (MQ=19)
                                                 tGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCaa                                  <  1:2982150/55‑1 (MQ=16)
                                                 tGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAg                    <  1:2971277/69‑1 (MQ=255)
                                                   gTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGa                 <  1:1116822/70‑1 (MQ=255)
                                                    tCGATCCGCTGGGCGGCATTAACACTCTGTGGGCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATg               >  1:205137/1‑71 (MQ=255)
                                                    tCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATg               >  1:1063233/1‑71 (MQ=255)
                                                     cGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCt                                                 <  1:773005/36‑1 (MQ=255)
                                                        tCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGgcgc           <  1:2405604/71‑1 (MQ=255)
                                                        tCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGgcgc           <  1:83250/71‑1 (MQ=255)
                                                         ccGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCt          >  1:3128460/1‑71 (MQ=255)
                                                          cGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCt          <  1:347877/70‑1 (MQ=255)
                                                               ggcggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATg      <  1:435572/69‑1 (MQ=255)
                                                               ggcggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGc     <  1:1447753/70‑1 (MQ=255)
                                                                 cggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGctct  >  1:2905870/1‑71 (MQ=255)
                                                                     aTTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGAt                >  1:961338/1‑53 (MQ=255)
                                                                     aTTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGa        <  1:2797230/61‑1 (MQ=255)
                                                                     |                                                                  
GCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCT  >  minE/386351‑386486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: