Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 386,449 T→C 8.2% I553I (ATT→ATC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE386,4490TC 8.2% 201.8 / 7.8 74I553I (ATT→ATCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (29/38);  new base C (3/3);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

GCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGT  >  minE/386379‑386515
                                                                      |                                                                  
gCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTAtt                                                                    <  1:1400524/71‑1 (MQ=255)
  tACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                                                  >  1:1419834/1‑71 (MQ=255)
   aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCGGCTGTTTGGTATTGc                                                                  >  1:149171/1‑70 (MQ=255)
   aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                                                  >  1:3195728/1‑70 (MQ=255)
   aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                                                  >  1:141739/1‑70 (MQ=255)
    cTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGcc                                                                 >  1:1986492/1‑70 (MQ=255)
     ttCCTCCATCAGGGCGTGGTCGATCCGCCGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCaa                                                               >  1:2960936/1‑71 (MQ=255)
      tcctccATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCaa                                                               >  1:2481852/1‑70 (MQ=255)
          ccATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAAc                                                              >  1:2955634/1‑67 (MQ=255)
           cATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGAt                                                         <  1:155986/71‑1 (MQ=255)
            aTCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATg                                                        <  1:665327/71‑1 (MQ=255)
             tCAGGGCGTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATg                                                        <  1:1210251/70‑1 (MQ=255)
             tCAGGGCGTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATGc                                                       >  1:2737037/1‑71 (MQ=255)
             tCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAAc                                                              >  1:2183047/1‑64 (MQ=255)
             tCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGc                                                       <  1:2140766/71‑1 (MQ=255)
                 ggCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGc                                                   <  1:452322/71‑1 (MQ=255)
                    gTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATGCt                                                      <  1:1683315/65‑1 (MQ=19)
                     tGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCaa                                                               <  1:2982150/55‑1 (MQ=16)
                     tGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAg                                                 <  1:2971277/69‑1 (MQ=255)
                       gTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGa                                              <  1:1116822/70‑1 (MQ=255)
                        tCGATCCGCTGGGCGGCATTAACACTCTGTGGGCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATg                                            >  1:205137/1‑71 (MQ=255)
                        tCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATg                                            >  1:1063233/1‑71 (MQ=255)
                            tCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGgcgc                                        <  1:83250/71‑1 (MQ=255)
                            tCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGgcgc                                        <  1:2405604/71‑1 (MQ=255)
                             ccGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCt                                       >  1:3128460/1‑71 (MQ=255)
                              cGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCt                                       <  1:347877/70‑1 (MQ=255)
                                   ggcggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATg                                   <  1:435572/69‑1 (MQ=255)
                                   ggcggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGc                                  <  1:1447753/70‑1 (MQ=255)
                                     cggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGctct                               >  1:2905870/1‑71 (MQ=255)
                                         aTTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGAt                                             >  1:961338/1‑53 (MQ=255)
                                         aTTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGa                                     <  1:2797230/61‑1 (MQ=255)
                                          ttAACACTCTGTGGCCGCTGTTTGGTATTGCCAAcc                                                             <  1:2049916/36‑1 (MQ=255)
                                           tAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                         >  1:362669/1‑71 (MQ=255)
                                           tAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                         >  1:2927949/1‑71 (MQ=255)
                                            aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                        >  1:3207140/1‑71 (MQ=255)
                                            aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                        >  1:1287479/1‑71 (MQ=255)
                                            aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                         <  1:85649/70‑1 (MQ=255)
                                            aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                         <  1:3513592/70‑1 (MQ=255)
                                             acacTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGctct                               >  1:3667578/1‑63 (MQ=255)
                                             acacTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGct                                 <  1:1234567/61‑1 (MQ=255)
                                             acacTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                        <  1:85950/70‑1 (MQ=255)
                                                ctctGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtggtg                    >  1:2255004/1‑71 (MQ=255)
                                                 tctGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                        <  1:3442788/66‑1 (MQ=255)
                                                   tgtgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGtgttg                 >  1:2659035/1‑71 (MQ=255)
                                                     tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                        <  1:2528438/62‑1 (MQ=255)
                                                     tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGgtgt                   <  1:3673804/67‑1 (MQ=255)
                                                     tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGgtgt                   <  1:775835/67‑1 (MQ=255)
                                                     tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGtgtt                  <  1:2875487/68‑1 (MQ=255)
                                                       gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtg                       <  1:1757540/61‑1 (MQ=255)
                                                       gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtg                       <  1:371071/61‑1 (MQ=255)
                                                       gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtg                       <  1:3615884/61‑1 (MQ=255)
                                                       gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTc              <  1:1704967/70‑1 (MQ=255)
                                                       gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTc              <  1:1771749/70‑1 (MQ=255)
                                                       gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCa             <  1:1116766/71‑1 (MQ=255)
                                                        ccGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCaa            <  1:73512/71‑1 (MQ=255)
                                                         cGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAg           <  1:3949117/71‑1 (MQ=255)
                                                         atcTGGTTGGCAATACCAAACAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTc              <  1:361100/66‑1 (MQ=25)
                                                           cTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt         <  1:2858353/71‑1 (MQ=255)
                                                           cTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt         >  1:2967973/1‑71 (MQ=255)
                                                            tGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt         >  1:1766780/1‑70 (MQ=255)
                                                            tGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt         >  1:3799247/1‑70 (MQ=255)
                                                             gTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGtgttgt                <  1:3794467/62‑1 (MQ=255)
                                                             gTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCa             >  1:2341087/1‑65 (MQ=255)
                                                              tttGGTATTGCCAACCAGATGCTGGCAGGGATGgcg                                         <  1:252441/36‑1 (MQ=255)
                                                              tttGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGa       <  1:2426886/70‑1 (MQ=255)
                                                              tttGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGa       >  1:2812340/1‑70 (MQ=255)
                                                               ttGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGaaa     >  1:3664869/1‑71 (MQ=255)
                                                                tGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGgtgt                   <  1:2283620/56‑1 (MQ=255)
                                                                tGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGaaa     >  1:2365144/1‑70 (MQ=255)
                                                                tGGTATAGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAAc    >  1:508265/1‑71 (MQ=255)
                                                                cggTATCTCCAACCAGATGCTGGCAGCCGTAGCGCTGGTACTGGGCACCGTTGTGCTGATTAAGATGaagc    >  1:3028141/2‑69 (MQ=255)
                                                                cggTATCTCCAACCAGATGCTGGCAGCCGTAGCGCTGGTACTGGGCACCGTTGTGCTGATTAAGATGaagc    >  1:2258643/2‑69 (MQ=255)
                                                                 ggTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCtg                              <  1:2734895/44‑1 (MQ=255)
                                                                  gTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAAc    <  1:3522920/69‑1 (MQ=255)
                                                                  gTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGt  >  1:2154366/1‑71 (MQ=255)
                                                                      |                                                                  
GCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGT  >  minE/386379‑386515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: