Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 386,450 G→T 8.1% A554S (GCC→TCC)  cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE386,4500GT 8.1% 237.2 / 4.8 74A554S (GCC→TCC) cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (30/37);  new base T (3/3);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACG  >  minE/386381‑386520
                                                                     |                                                                      
tACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                                                       >  1:1419834/1‑71 (MQ=255)
 aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCGGCTGTTTGGTATTGc                                                                       >  1:149171/1‑70 (MQ=255)
 aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                                                       >  1:3195728/1‑70 (MQ=255)
 aCTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGc                                                                       >  1:141739/1‑70 (MQ=255)
  cTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGcc                                                                      >  1:1986492/1‑70 (MQ=255)
   ttCCTCCATCAGGGCGTGGTCGATCCGCCGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCaa                                                                    >  1:2960936/1‑71 (MQ=255)
    tcctccATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCaa                                                                    >  1:2481852/1‑70 (MQ=255)
        ccATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAAc                                                                   >  1:2955634/1‑67 (MQ=255)
         cATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGAt                                                              <  1:155986/71‑1 (MQ=255)
          aTCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATg                                                             <  1:665327/71‑1 (MQ=255)
           tCAGGGCGTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATg                                                             <  1:1210251/70‑1 (MQ=255)
           tCAGGGCGTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATGc                                                            >  1:2737037/1‑71 (MQ=255)
           tCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAAc                                                                   >  1:2183047/1‑64 (MQ=255)
           tCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGc                                                            <  1:2140766/71‑1 (MQ=255)
               ggCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGc                                                        <  1:452322/71‑1 (MQ=255)
                  gTGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCAACCAGATGCt                                                           <  1:1683315/65‑1 (MQ=19)
                   tGGTTGATCCGCTGGGCGGCGTTAAGAGCCTGTGGCCGCTGTTCGGTATCTCCaa                                                                    <  1:2982150/55‑1 (MQ=16)
                   tGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAg                                                      <  1:2971277/69‑1 (MQ=255)
                     gTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGa                                                   <  1:1116822/70‑1 (MQ=255)
                      tCGATCCGCTGGGCGGCATTAACACTCTGTGGGCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATg                                                 >  1:205137/1‑71 (MQ=255)
                      tCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATg                                                 >  1:1063233/1‑71 (MQ=255)
                          tCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGgcgc                                             <  1:83250/71‑1 (MQ=255)
                          tCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGgcgc                                             <  1:2405604/71‑1 (MQ=255)
                           ccGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCt                                            >  1:3128460/1‑71 (MQ=255)
                            cGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCt                                            <  1:347877/70‑1 (MQ=255)
                                 ggcggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATg                                        <  1:435572/69‑1 (MQ=255)
                                 ggcggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGc                                       <  1:1447753/70‑1 (MQ=255)
                                   cggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGctct                                    >  1:2905870/1‑71 (MQ=255)
                                       aTTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGAt                                                  >  1:961338/1‑53 (MQ=255)
                                       aTTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGa                                          <  1:2797230/61‑1 (MQ=255)
                                        ttAACACTCTGTGGCCGCTGTTTGGTATTGCCAAcc                                                                  <  1:2049916/36‑1 (MQ=255)
                                         tAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                              >  1:2927949/1‑71 (MQ=255)
                                         tAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                              >  1:362669/1‑71 (MQ=255)
                                          aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                             >  1:3207140/1‑71 (MQ=255)
                                          aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                             >  1:1287479/1‑71 (MQ=255)
                                          aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                              <  1:85649/70‑1 (MQ=255)
                                          aaCACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCg                              <  1:3513592/70‑1 (MQ=255)
                                           acacTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGctct                                    >  1:3667578/1‑63 (MQ=255)
                                           acacTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGct                                      <  1:1234567/61‑1 (MQ=255)
                                           acacTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                             <  1:85950/70‑1 (MQ=255)
                                              ctctGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtggtg                         >  1:2255004/1‑71 (MQ=255)
                                               tctGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                             <  1:3442788/66‑1 (MQ=255)
                                                 tgtgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGtgttg                      >  1:2659035/1‑71 (MQ=255)
                                                   tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgt                             <  1:2528438/62‑1 (MQ=255)
                                                   tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGgtgt                        <  1:3673804/67‑1 (MQ=255)
                                                   tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGgtgt                        <  1:775835/67‑1 (MQ=255)
                                                   tgGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGtgtt                       <  1:2875487/68‑1 (MQ=255)
                                                     gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtg                            <  1:1757540/61‑1 (MQ=255)
                                                     gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtg                            <  1:3615884/61‑1 (MQ=255)
                                                     gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCgtg                            <  1:371071/61‑1 (MQ=255)
                                                     gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTc                   <  1:1704967/70‑1 (MQ=255)
                                                     gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTc                   <  1:1771749/70‑1 (MQ=255)
                                                     gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCa                  <  1:1116766/71‑1 (MQ=255)
                                                      ccGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCaa                 <  1:73512/71‑1 (MQ=255)
                                                       cGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAg                <  1:3949117/71‑1 (MQ=255)
                                                       atcTGGTTGGCAATACCAAACAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTc                   <  1:361100/66‑1 (MQ=25)
                                                         cTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt              >  1:2967973/1‑71 (MQ=255)
                                                         cTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt              <  1:2858353/71‑1 (MQ=255)
                                                          tGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt              >  1:1766780/1‑70 (MQ=255)
                                                          tGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGAt              >  1:3799247/1‑70 (MQ=255)
                                                           gTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGtgttgt                     <  1:3794467/62‑1 (MQ=255)
                                                           gTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCa                  >  1:2341087/1‑65 (MQ=255)
                                                            tttGGTATTGCCAACCAGATGCTGGCAGGGATGgcg                                              <  1:252441/36‑1 (MQ=255)
                                                            tttGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGa            <  1:2426886/70‑1 (MQ=255)
                                                            tttGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGa            >  1:2812340/1‑70 (MQ=255)
                                                             ttGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGaaa          >  1:3664869/1‑71 (MQ=255)
                                                              tGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGgtgt                        <  1:2283620/56‑1 (MQ=255)
                                                              tGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGaaa          >  1:2365144/1‑70 (MQ=255)
                                                              tGGTATAGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAAc         >  1:508265/1‑71 (MQ=255)
                                                              cggTATCTCCAACCAGATGCTGGCAGCCGTAGCGCTGGTACTGGGCACCGTTGTGCTGATTAAGATGaagc         >  1:3028141/2‑69 (MQ=255)
                                                              cggTATCTCCAACCAGATGCTGGCAGCCGTAGCGCTGGTACTGGGCACCGTTGTGCTGATTAAGATGaagc         >  1:2258643/2‑69 (MQ=255)
                                                               ggTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCtg                                   <  1:2734895/44‑1 (MQ=255)
                                                                gTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAAc         <  1:3522920/69‑1 (MQ=255)
                                                                gTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGt       >  1:2154366/1‑71 (MQ=255)
                                                                     gCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACg        >  1:48674/1‑65 (MQ=255)
                                                                     gCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACg  >  1:2235627/1‑71 (MQ=255)
                                                                     gCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACg  >  1:3450580/1‑71 (MQ=255)
                                                                     |                                                                      
TACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACG  >  minE/386381‑386520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: