| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 412,492 | C→T | 30.2% | intergenic (‑137/+152) | leuS ← / ← lnt | leucyl‑tRNA synthetase/apolipoprotein N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 412,492 | 0 | C | T | 30.2% | 71.4 / 37.7 | 53 | intergenic (‑137/+152) | leuS/lnt | leucyl‑tRNA synthetase/apolipoprotein N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (23/14); new base T (7/9); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTA > minE/412422‑412556 | atagcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTAc > 1:2810055/1‑71 (MQ=255) agcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACt > 1:1021918/1‑70 (MQ=255) agcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACt > 1:3552510/1‑70 (MQ=255) gcatagcCCAACCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCa < 1:1183311/71‑1 (MQ=255) catagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCAt < 1:1284544/71‑1 (MQ=255) atagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATa < 1:809509/71‑1 (MQ=255) agcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACa > 1:13772/1‑71 (MQ=255) gcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACAt > 1:1101917/1‑71 (MQ=255) aaCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAAtt < 1:1954904/71‑1 (MQ=255) aaCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAAtt > 1:1377019/1‑71 (MQ=255) cGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTa > 1:3537079/1‑71 (MQ=255) tCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACa < 1:3430932/71‑1 (MQ=255) ccGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTa > 1:3499734/1‑68 (MQ=255) ccGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTa > 1:2881400/1‑68 (MQ=255) gcgTCAAAACAGCCTTTCTCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACagag < 1:2541320/71‑1 (MQ=255) cgTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGt > 1:3150975/1‑71 (MQ=255) gTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGtt < 1:2919932/71‑1 (MQ=255) aaaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTtg < 1:3689069/70‑1 (MQ=255) aaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTa < 1:3162454/60‑1 (MQ=255) aaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTtgt < 1:3280688/70‑1 (MQ=255) aaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTgg > 1:1161618/1‑71 (MQ=255) aCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTgg < 1:3267662/70‑1 (MQ=255) cAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGc > 1:1918398/1‑71 (MQ=255) cAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGc > 1:2408743/1‑71 (MQ=255) gCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACa < 1:280929/47‑1 (MQ=255) tttCGCTCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTa < 1:1660098/52‑1 (MQ=255) ttCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGt < 1:1546245/67‑1 (MQ=255) ttCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTAt > 1:3513378/1‑69 (MQ=255) ttCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTAt > 1:1468552/1‑69 (MQ=255) tCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTa < 1:1640367/70‑1 (MQ=255) gcACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTagcaa < 1:1851322/70‑1 (MQ=255) tCGACGTTGAAATGATGCCCGGATTATTCATACATTAAtt > 1:3183275/1‑40 (MQ=255) gACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTagcaaagcaa < 1:1226502/69‑1 (MQ=255) gACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAg > 1:1595881/1‑70 (MQ=255) aCGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTtg < 1:3684642/48‑1 (MQ=255) ttGAAATGATGCCCGGATTATTCATACATTAATTTACATAGTTTGTGGGCGTAt < 1:1686327/54‑1 (MQ=255) ttGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAAc > 1:3819199/1‑70 (MQ=255) tGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACa > 1:72579/1‑70 (MQ=255) aaTGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTa > 1:3185541/1‑52 (MQ=255) aaTGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTa > 1:1721581/1‑52 (MQ=255) aaTGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAg < 1:2705431/70‑1 (MQ=255) aaTGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAg > 1:3872541/1‑70 (MQ=255) tgatgCCCGGATTACTCATACATTAATTTACAGAGTTtg < 1:2849826/39‑1 (MQ=255) tgatgCCCGGATTACTCATACATTAATTTACAGAGTTtg < 1:2172983/39‑1 (MQ=255) gatgCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACg > 1:1516782/1‑71 (MQ=255) gatgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACg > 1:1546484/1‑71 (MQ=255) gatgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACg > 1:3797784/1‑71 (MQ=255) gatgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACg > 1:3177949/1‑71 (MQ=255) atgCCCGGATTACTCATACATTAATTTACAGAGTTtgtg > 1:1472111/1‑39 (MQ=255) atgCCCGGATTACTCATACATTAATTTACAGAGTTtgtg > 1:746308/1‑39 (MQ=255) atgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACATAGAACg < 1:4791/70‑1 (MQ=255) ggcttACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCtatta > 1:1433436/4‑71 (MQ=255) ggATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGCACGTCtatta > 1:3195956/1‑71 (MQ=255) ggATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCtatta > 1:395824/1‑71 (MQ=255) | ATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTA > minE/412422‑412556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |