| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 418,094 | C→T | 18.9% | A55T (GCT→ACT) | miaB ← | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 418,094 | 0 | C | T | 18.9% | 125.4 / 22.0 | 58 | A55T (GCT→ACT) | miaB | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Reads supporting (aligned to +/- strand): ref base C (27/20); new base T (7/4); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01 | |||||||||||
TAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCC > minE/418025‑418166 | tAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGcc > 1:1380386/1‑71 (MQ=255) aGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTtctc < 1:2288230/71‑1 (MQ=255) gTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgc < 1:1038452/71‑1 (MQ=255) gATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt > 1:315252/1‑71 (MQ=255) ttctCTTTTAACAGTTTCCAGCGACCCAACTGATTGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:3280437/1‑69 (MQ=255) ttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGa > 1:2194322/1‑68 (MQ=255) ctctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAgg > 1:772600/1‑71 (MQ=255) tctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGgt > 1:1140102/1‑71 (MQ=255) ttttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGgtgt > 1:2532081/1‑71 (MQ=255) ttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGgtg < 1:153746/68‑1 (MQ=255) ttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGgtg < 1:1175868/68‑1 (MQ=255) aCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagc < 1:3513740/71‑1 (MQ=255) aCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcag > 1:1852091/1‑70 (MQ=255) aCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcag < 1:2120492/70‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:3932344/1‑70 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:2305042/1‑70 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCtt > 1:2561200/1‑42 (MQ=255) ttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:3247712/1‑54 (MQ=255) tCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcagcag > 1:2512855/1‑70 (MQ=255) tCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagcag > 1:1615283/1‑70 (MQ=255) gCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACAt < 1:3861403/71‑1 (MQ=255) cGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATc > 1:983126/1‑71 (MQ=255) ccAACTGATGGAGGACTTTTTCCTGAGCATTCTCGCGGATTGAGCatg > 1:1615649/1‑46 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct > 1:584972/1‑71 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct < 1:1277958/71‑1 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg < 1:569519/40‑1 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg < 1:240913/40‑1 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagc > 1:3808039/1‑55 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttctt < 1:12708/71‑1 (MQ=255) aTGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCg > 1:3630372/1‑71 (MQ=255) aTGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCg > 1:3001757/1‑71 (MQ=255) aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttctt < 1:2238280/68‑1 (MQ=255) aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCttcttc < 1:2941882/69‑1 (MQ=255) aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCg > 1:1073148/1‑71 (MQ=255) tGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGAGTTcagcagcag > 1:1439379/1‑53 (MQ=255) gAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGcc < 1:2577980/70‑1 (MQ=255) gAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGcc < 1:855586/70‑1 (MQ=255) aGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACg > 1:1791439/1‑71 (MQ=255) aGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct < 1:2089315/62‑1 (MQ=255) aGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACg < 1:2376508/71‑1 (MQ=255) gACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGtt < 1:1760460/39‑1 (MQ=255) gACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt < 1:719346/71‑1 (MQ=255) gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt > 1:2622032/1‑71 (MQ=255) aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTc > 1:758344/1‑71 (MQ=255) tttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCgg > 1:1991568/1‑71 (MQ=255) tttCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:3730590/1‑70 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt < 1:3833203/70‑1 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:3911314/1‑70 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:3152349/1‑70 (MQ=255) ttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:721199/1‑69 (MQ=255) ccTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACg > 1:727309/1‑62 (MQ=255) cTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGtt < 1:19890/71‑1 (MQ=255) cTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGtt < 1:1217395/71‑1 (MQ=255) cTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGt < 1:3667299/70‑1 (MQ=255) tGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttc < 1:3809149/50‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttctt > 1:2510071/1‑50 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTg > 1:343976/1‑69 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGa > 1:2330112/1‑70 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGa > 1:1961010/1‑70 (MQ=255) ccTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTC‑‑CCACGTCGGTCAGTTGATAGcc < 1:657038/71‑1 (MQ=255) | TAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCC > minE/418025‑418166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |