| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 423,971 | T→C | 21.7% | S309G (AGT→GGT) | nagC ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 423,971 | 0 | T | C | 21.7% | 113.2 / 28.6 | 60 | S309G (AGT→GGT) | nagC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (24/23); new base C (7/6); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
TTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGG > minE/423904‑424038 | ttGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATc > 1:433765/1‑71 (MQ=255) aTAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGc < 1:2279430/70‑1 (MQ=255) tAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCt < 1:1630812/71‑1 (MQ=255) aTGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGt < 1:2019439/70‑1 (MQ=255) tGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTc > 1:2472195/1‑71 (MQ=255) ggCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTcg > 1:2412007/1‑71 (MQ=255) ggCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTcg > 1:1834528/1‑71 (MQ=255) gCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTcgc > 1:2469192/1‑71 (MQ=255) cGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTcgcg > 1:1804353/1‑71 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCgg < 1:213685/71‑1 (MQ=255) gTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTg < 1:768270/60‑1 (MQ=255) gTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTg < 1:763649/60‑1 (MQ=255) ttttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTg > 1:3603038/1‑71 (MQ=255) ttttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTc < 1:2768108/62‑1 (MQ=255) tttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGtt > 1:1496182/1‑60 (MQ=255) tACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCa < 1:3760757/70‑1 (MQ=255) tACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGc > 1:1196525/1‑69 (MQ=255) aCCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAg < 1:3334144/70‑1 (MQ=255) cctgATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAg < 1:527711/65‑1 (MQ=255) aGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGt > 1:3287182/1‑71 (MQ=255) gATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt > 1:3329528/1‑71 (MQ=255) tGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGa > 1:845777/1‑64 (MQ=255) tGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGa > 1:841139/1‑64 (MQ=255) gaccgacATACTCAATTACTTCCGACGCCAGACTATCGcc < 1:152755/40‑1 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATgg > 1:189144/1‑71 (MQ=255) cgacATACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTg < 1:2557853/71‑1 (MQ=255) gacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCtt < 1:3171320/50‑1 (MQ=255) gacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:1866886/63‑1 (MQ=255) cATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGa > 1:65431/1‑63 (MQ=255) cATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGa > 1:1286508/1‑63 (MQ=255) aTACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgt > 1:2998021/1‑71 (MQ=255) aTACTCAATTACTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgt > 1:2423606/1‑71 (MQ=255) tACTCAATTACTTCCGACGCCAGACTATCGCCTTTg < 1:1146597/36‑1 (MQ=255) tACTCAATTACTTCCGACGCCAGACTATCGCCTTTg < 1:2873527/36‑1 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtcgt > 1:1072843/1‑71 (MQ=255) aaTTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtcgtc < 1:981787/69‑1 (MQ=255) aaTTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtcgtc < 1:3374289/69‑1 (MQ=255) aaTTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTcc < 1:2575503/70‑1 (MQ=255) aTTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCa < 1:825568/70‑1 (MQ=255) ttACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAg > 1:1744229/1‑70 (MQ=255) ttACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGc > 1:86332/1‑71 (MQ=255) ttACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGc > 1:3283930/1‑71 (MQ=255) aCTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCg < 1:2095931/70‑1 (MQ=255) aCTTCCGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCgg > 1:3931347/1‑71 (MQ=255) cTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtc < 1:290946/61‑1 (MQ=255) ttCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2387608/48‑1 (MQ=255) ccGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCa < 1:3037011/69‑1 (MQ=255) ccGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCAcg > 1:1815423/1‑71 (MQ=255) ccGACGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCAc < 1:462742/70‑1 (MQ=255) cGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCAcg > 1:1582954/1‑70 (MQ=255) aCGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGa > 1:2753791/1‑45 (MQ=255) aCGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCgg > 1:2280363/1‑71 (MQ=255) aCGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGAt < 1:3776582/37‑1 (MQ=255) aCGCCAGACCATCGCCTTTGTTCGCGGCTTTGCAGAt < 1:2056184/37‑1 (MQ=255) cAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAg > 1:641527/1‑49 (MQ=255) gACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:509221/37‑1 (MQ=255) gACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:3083784/37‑1 (MQ=255) gACCATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtcgt > 1:1363561/1‑51 (MQ=255) aCTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTgg > 1:2612048/1‑70 (MQ=255) cTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTgg > 1:3686377/1‑69 (MQ=255) | TTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGG > minE/423904‑424038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |