| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 425,152 | C→T | 27.0% | D301N (GAT→AAT) | nagA ← | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 425,152 | 0 | C | T | 27.0% | 115.7 / 48.2 | 74 | D301N (GAT→AAT) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base C (21/33); new base T (7/13); total (28/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTG > minE/425082‑425220 | cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:1862216/1‑71 (MQ=255) cAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTc < 1:3922405/71‑1 (MQ=255) aGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTcc < 1:1903632/71‑1 (MQ=255) aTTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt < 1:655560/71‑1 (MQ=255) ttACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTTATCCACACAAAGTCCGt < 1:288176/70‑1 (MQ=255) ttACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt > 1:2158650/1‑70 (MQ=255) cgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTAc < 1:86993/70‑1 (MQ=255) cgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTAc < 1:126793/70‑1 (MQ=255) gcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACgg < 1:2752018/71‑1 (MQ=255) gcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACgg < 1:69855/71‑1 (MQ=255) acgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTc > 1:3012552/1‑61 (MQ=255) acgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta < 1:1064065/71‑1 (MQ=255) acgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta < 1:405375/71‑1 (MQ=255) cgcCTTCTATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta < 1:2869552/70‑1 (MQ=255) cgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAg < 1:1432254/58‑1 (MQ=255) gcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtag > 1:1989292/1‑70 (MQ=255) cTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtagta > 1:583595/1‑70 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGgta > 1:3802358/1‑65 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACTGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:2092180/71‑1 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:221455/71‑1 (MQ=255) aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:1778322/70‑1 (MQ=255) aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGt < 1:3478509/71‑1 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGttt > 1:2903815/1‑71 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:581633/71‑1 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTc > 1:738713/1‑49 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:1549037/71‑1 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:1730783/71‑1 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:1612156/71‑1 (MQ=255) gTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:3207996/1‑35 (MQ=255) gTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTa > 1:27953/1‑51 (MQ=255) ttAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTa < 1:1997892/68‑1 (MQ=255) aGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtt < 1:1286553/62‑1 (MQ=255) aGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGc > 1:56251/1‑70 (MQ=255) aGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGc > 1:2998155/1‑70 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGgta < 1:2579933/51‑1 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGgta < 1:928696/51‑1 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGgta < 1:1042416/51‑1 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:3456404/63‑1 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:1626983/63‑1 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCa > 1:1073497/1‑70 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGgta < 1:2555447/49‑1 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGgta < 1:892325/49‑1 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACaaa > 1:639036/1‑36 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACaa > 1:2832508/1‑35 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGttt > 1:2977845/1‑60 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGttt > 1:2624275/1‑60 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaa < 1:2082678/71‑1 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaa < 1:1306900/71‑1 (MQ=255) gAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAAt > 1:1127309/1‑71 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:3885590/71‑1 (MQ=255) aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:2929655/70‑1 (MQ=255) cTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTa > 1:138988/1‑54 (MQ=255) cTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACg < 1:626918/38‑1 (MQ=255) cTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTatat > 1:1456824/1‑47 (MQ=255) tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaa > 1:3295265/1‑61 (MQ=255) tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGtt > 1:1153002/1‑71 (MQ=255) aaCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGtt < 1:1360069/70‑1 (MQ=255) aCGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTc < 1:3703845/70‑1 (MQ=255) cGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaa < 1:1437566/58‑1 (MQ=255) cGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCa > 1:2860562/1‑55 (MQ=255) cGTACCCTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCa > 1:510316/1‑55 (MQ=255) gTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:958847/43‑1 (MQ=255) gTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:1468536/60‑1 (MQ=255) gTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:3816860/60‑1 (MQ=255) tACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTAccc < 1:126074/50‑1 (MQ=255) tACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATg < 1:555778/71‑1 (MQ=255) aCCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGaa > 1:2287031/1‑60 (MQ=255) ccGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGc > 1:3465072/1‑50 (MQ=255) ccGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGt < 1:123639/70‑1 (MQ=255) cGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTg > 1:3003326/1‑71 (MQ=255) ttCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:2930526/54‑1 (MQ=255) ttCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGc < 1:3406714/71‑1 (MQ=255) ctcATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCAc > 1:2183575/1‑71 (MQ=255) ctcATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCAc > 1:2670158/1‑71 (MQ=255) tcATCCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:2835507/38‑1 (MQ=255) aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACctg > 1:2018064/1‑71 (MQ=255) | CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTG > minE/425082‑425220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |