| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 434,040 | C→T | 20.4% | intergenic (‑93/+196) | fur ← / ← fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 434,040 | 0 | C | T | 20.4% | 111.8 / 22.5 | 54 | intergenic (‑93/+196) | fur/fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Reads supporting (aligned to +/- strand): ref base C (27/16); new base T (4/7); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
ACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAA > minE/433977‑434109 | aCTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTaa < 1:1842672/70‑1 (MQ=255) cTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAAt > 1:826344/1‑71 (MQ=255) ttCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCAc < 1:744299/62‑1 (MQ=255) ttCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAAt > 1:3193989/1‑70 (MQ=255) cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGc > 1:3670245/1‑62 (MQ=255) cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa > 1:2127251/1‑70 (MQ=255) aTTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaa < 1:1803114/70‑1 (MQ=255) gaagaaGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAcc < 1:1930052/70‑1 (MQ=255) gaagTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACaa < 1:3056422/71‑1 (MQ=255) aagTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAg > 1:1645647/1‑71 (MQ=255) gTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCAc > 1:3155798/1‑49 (MQ=255) tGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc < 1:171630/71‑1 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc < 1:2589069/70‑1 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc < 1:2589974/70‑1 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCt > 1:3874686/1‑71 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCACt > 1:2988077/1‑71 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaa < 1:2801211/52‑1 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc < 1:2497498/70‑1 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCt > 1:3276945/1‑71 (MQ=255) aGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTgg > 1:1452451/1‑71 (MQ=255) aGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTgg > 1:3668141/1‑71 (MQ=255) gAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc > 1:2449317/1‑66 (MQ=255) aaTTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGc > 1:2284710/1‑70 (MQ=255) aaTTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGc > 1:1973637/1‑70 (MQ=255) aaTTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCa < 1:21083/71‑1 (MQ=255) aaTTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCa < 1:3839987/71‑1 (MQ=255) ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt > 1:2567255/1‑70 (MQ=255) tGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt > 1:3183985/1‑69 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt > 1:2546663/1‑66 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaaa > 1:3809828/1‑44 (MQ=255) gaTAATGAGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat > 1:1235505/1‑63 (MQ=255) aTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa < 1:1758173/71‑1 (MQ=255) aTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCa > 1:2683460/1‑54 (MQ=255) aTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaa < 1:3292223/70‑1 (MQ=255) tGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc > 1:170418/1‑71 (MQ=255) gCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt > 1:282383/1‑58 (MQ=255) cGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACg < 1:2969514/70‑1 (MQ=255) tATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGt < 1:1844917/41‑1 (MQ=255) aTCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCACt > 1:1365728/1‑44 (MQ=255) tCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgaat > 1:184715/1‑68 (MQ=255) cATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa < 1:2387722/60‑1 (MQ=255) aTTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGcac < 1:1912007/70‑1 (MQ=255) aTTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca > 1:1744752/1‑69 (MQ=255) aTTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca > 1:3583159/1‑69 (MQ=255) ttATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAg < 1:1493181/71‑1 (MQ=255) ttATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAg > 1:2431517/1‑71 (MQ=255) ttATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAg > 1:2322199/1‑71 (MQ=255) ttATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAg > 1:3471661/1‑71 (MQ=255) tataGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGGACAg > 1:3713505/1‑70 (MQ=255) taGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCt < 1:947789/70‑1 (MQ=255) taGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCt < 1:952323/70‑1 (MQ=255) taGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta > 1:3396593/1‑51 (MQ=255) gAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTa > 1:2664680/1‑70 (MQ=255) gAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTAc < 1:3350299/71‑1 (MQ=255) ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat < 1:2390854/41‑1 (MQ=255) aCGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACaa < 1:1503887/71‑1 (MQ=255) | ACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAA > minE/433977‑434109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |