| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 452,736 | C→T | 17.8% | A234V (GCA→GTA) | phr → | deoxyribodipyrimidine photolyase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 452,736 | 0 | C | T | 17.8% | 99.6 / 14.6 | 45 | A234V (GCA→GTA) | phr | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base C (16/21); new base T (5/3); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTG > minE/452671‑452802 | cGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAg > 1:713759/1‑71 (MQ=255) cAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGcc < 1:365885/70‑1 (MQ=255) cAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCg > 1:446346/1‑71 (MQ=255) cAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCg < 1:3550235/71‑1 (MQ=255) cAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCg < 1:3910847/71‑1 (MQ=255) aGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGt < 1:1453546/71‑1 (MQ=255) aGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGt > 1:3588599/1‑71 (MQ=255) aGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGt > 1:3584196/1‑71 (MQ=255) gCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTc < 1:1735815/70‑1 (MQ=255) ccAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCg < 1:2811370/70‑1 (MQ=255) ccAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCgg < 1:1401696/71‑1 (MQ=255) cAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGc < 1:199782/71‑1 (MQ=255) aCGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCAc > 1:1204319/1‑52 (MQ=255) cGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGcc < 1:1498494/71‑1 (MQ=255) cGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGcc < 1:3329216/71‑1 (MQ=255) ggTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCg > 1:1486226/1‑63 (MQ=255) ggTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCt < 1:920051/71‑1 (MQ=255) gTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCt > 1:3882247/1‑70 (MQ=255) gCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTgg > 1:1407245/1‑70 (MQ=255) cGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGc < 1:3065150/43‑1 (MQ=255) cGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGt < 1:3911745/52‑1 (MQ=255) cGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTg < 1:2520568/55‑1 (MQ=255) gagaATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAcg > 1:2998523/1‑71 (MQ=255) agaATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAcgg > 1:2845571/1‑71 (MQ=255) agaATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAcgg > 1:989566/1‑71 (MQ=255) tatGAGCAACAACGAGATTTTCCGGCAGTGGAAGGTACCAGc < 1:2687481/42‑1 (MQ=255) tGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGt > 1:1257126/1‑71 (MQ=255) tGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGt > 1:858403/1‑71 (MQ=255) gAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGt > 1:89345/1‑70 (MQ=255) gCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTg < 1:2331408/43‑1 (MQ=255) gCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTg < 1:2258405/43‑1 (MQ=255) gCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTg < 1:521284/43‑1 (MQ=255) acaacGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgc < 1:1155460/71‑1 (MQ=255) caacGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgcc > 1:2531271/1‑71 (MQ=255) caacGAGATTTTCCGGCAGTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgcc > 1:795254/1‑71 (MQ=255) cGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgcctcg < 1:583063/71‑1 (MQ=255) cGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgc > 1:478744/1‑67 (MQ=255) aTTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAcggg < 1:1763976/52‑1 (MQ=255) ttttCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcg > 1:3123005/1‑61 (MQ=255) tCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGc < 1:3329512/70‑1 (MQ=255) cGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTg < 1:2730293/71‑1 (MQ=255) cGGCAGTGGAAGGCACCAGCCGTTT‑TCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTg < 1:1685301/70‑1 (MQ=255) gTGGAAGGTACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCg > 1:2536988/1‑71 (MQ=255) gTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTg < 1:930172/62‑1 (MQ=255) aaGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCttg > 1:244560/1‑71 (MQ=255) aGGTACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCtt > 1:1772084/1‑60 (MQ=255) | CGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTG > minE/452671‑452802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |