Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	454,680	A→T	7.7%	R135R (CGT→CGA)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	454,680	0	A	T	7.7%	243.0 / 10.4	91	R135R (CGT→CGA)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (45/39);  new base T (5/2);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

CACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCG  >  minE/454610‑454749                                                                       |                                                                      cacaGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCacga                                                                       >  1:1204925/1‑71 (MQ=255)    aGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCacgacg                                                                     <  1:2568347/70‑1 (MQ=255)    aGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGAcgc                                                                    <  1:2857253/71‑1 (MQ=255)       cGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGa                                                                 <  1:2740831/71‑1 (MQ=255)        gATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGAt                                                                >  1:828148/1‑71 (MQ=255)          tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGAt                                                                <  1:1862105/69‑1 (MQ=255)          tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCg                                                              >  1:3902234/1‑71 (MQ=255)          tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCg                                                              >  1:1665292/1‑71 (MQ=255)            ggTGCGATAATAGACCCCACTTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                            <  1:549847/71‑1 (MQ=255)            ggTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                             <  1:900590/70‑1 (MQ=255)            ggTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                             <  1:1987309/70‑1 (MQ=255)             gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCgg                                                                  >  1:1140057/1‑64 (MQ=255)             gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                            <  1:524166/70‑1 (MQ=255)             gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                            <  1:3295401/70‑1 (MQ=255)                cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                        >  1:1172243/1‑71 (MQ=255)                cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                        >  1:1569829/1‑71 (MQ=255)                  ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGAcgcg                                                                   <  1:1952022/58‑1 (MQ=255)                   taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                     >  1:2161032/1‑71 (MQ=255)                    aataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                             >  1:1899291/1‑62 (MQ=255)                        gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                           >  1:2036705/1‑60 (MQ=255)                        gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                     <  1:184754/66‑1 (MQ=255)                           cccACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                   <  1:859548/65‑1 (MQ=255)                           cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc                                             >  1:3847756/1‑71 (MQ=255)                             cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGc                                           >  1:608391/1‑71 (MQ=255)                              aCGTAACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTg                                                          >  1:831538/1‑55 (MQ=255)                               cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                         <  1:1312282/71‑1 (MQ=255)                                gTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGa                                        <  1:2009645/71‑1 (MQ=255)                                 ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                       <  1:276893/71‑1 (MQ=255)                                   aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                       >  1:373191/1‑69 (MQ=255)                                   aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                       >  1:1799032/1‑69 (MQ=255)                                    cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                       >  1:2578201/1‑68 (MQ=255)                                    cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                     <  1:2559078/70‑1 (MQ=255)                                     ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                   >  1:2965736/1‑71 (MQ=255)                                     ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                   >  1:2391635/1‑71 (MQ=255)                                      cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                   >  1:3608211/1‑70 (MQ=255)                                       gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                          <  1:2241698/62‑1 (MQ=255)                                       gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                   >  1:3541892/1‑69 (MQ=255)                                       cccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCTGTTGGCTCATACAGCTCGcc                                          <  1:3619297/61‑1 (MQ=255)                                        cagcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                >  1:2326625/3‑71 (MQ=255)                                         cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGc                               >  1:2477066/1‑71 (MQ=255)                                          gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                              <  1:3281296/71‑1 (MQ=255)                                          gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                              >  1:405055/1‑71 (MQ=255)                                          gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                              <  1:829819/71‑1 (MQ=255)                                          gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                              >  1:1069030/1‑71 (MQ=255)                                          gcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                              >  1:3766968/1‑71 (MQ=255)                                           cATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCtt                             <  1:3802070/71‑1 (MQ=255)                                            aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                            <  1:1096568/71‑3 (MQ=255)                                              aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                               <  1:2158513/50‑1 (MQ=255)                                              aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACg                          >  1:3177783/1‑71 (MQ=255)                                              aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTAACg                          >  1:165886/1‑71 (MQ=255)                                               cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                         <  1:501747/71‑1 (MQ=255)                                               cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                         >  1:3877817/1‑71 (MQ=255)                                               cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                         <  1:1233663/71‑1 (MQ=255)                                                aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                  <  1:1081706/45‑1 (MQ=255)                                                  cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                         >  1:2540867/1‑68 (MQ=255)                                                  cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                         >  1:1202482/1‑68 (MQ=255)                                                   aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                         >  1:2064997/1‑67 (MQ=255)                                                    gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                     <  1:3110265/38‑1 (MQ=255)                                                    gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                              <  1:3103711/61‑1 (MQ=255)                                                     cGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                         >  1:2622821/1‑49 (MQ=255)                                                     cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                   >  1:3321801/1‑71 (MQ=255)                                                     cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                   >  1:402654/1‑71 (MQ=255)                                                      gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                       <  1:3643367/50‑1 (MQ=255)                                                      gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                   >  1:1724687/1‑70 (MQ=255)                                                       agaAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                     >  1:78781/1‑35 (MQ=255)                                                       agaAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                  >  1:2698670/1‑38 (MQ=255)                                                       agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                               <  1:3723749/41‑1 (MQ=255)                                                       agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                    >  1:1792505/1‑68 (MQ=255)                                                       aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttt                 <  1:407424/69‑2 (MQ=255)                                                        gaAACCGCCATCACGACGCGGATCGGTTGGCTCATTCAGCTCGCCGAGCAGACAGCTTACGTTAGAt                    >  1:2123033/1‑67 (MQ=255)                                                        gaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                <  1:559351/71‑1 (MQ=255)                                                           aCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGtt                        >  1:2765125/1‑60 (MQ=255)                                                           aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCg                                            >  1:1895018/1‑40 (MQ=255)                                                            ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg            <  1:1102261/71‑1 (MQ=255)                                                            ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg            <  1:683214/71‑1 (MQ=255)                                                             cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                              <  1:2614407/52‑1 (MQ=255)                                                             cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAgg           >  1:2730951/1‑71 (MQ=255)                                                             cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg            <  1:3693585/70‑1 (MQ=255)                                                             cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCAGAGCAGACAGCTTACGTTAGATTTAAACAgg           >  1:2724593/1‑71 (MQ=255)                                                              gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                    >  1:3615566/1‑61 (MQ=255)                                                              gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                    >  1:2097698/1‑61 (MQ=255)                                                               ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                              <  1:484344/50‑1 (MQ=255)                                                               ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                            >  1:968076/1‑52 (MQ=255)                                                               ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                            >  1:50847/1‑52 (MQ=255)                                                               ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                            >  1:783409/1‑52 (MQ=255)                                                                cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                     <  1:3866321/42‑1 (MQ=255)                                                                cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa        <  1:1801350/71‑1 (MQ=255)                                                                 aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGc                                      >  1:3345456/1‑40 (MQ=255)                                                                 aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt       <  1:813998/71‑1 (MQ=255)                                                                  tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa        >  1:246003/1‑69 (MQ=255)                                                                  tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa      <  1:2751043/71‑1 (MQ=255)                                                                  tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa      <  1:3780608/71‑1 (MQ=255)                                                                   cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACa             >  1:968035/1‑63 (MQ=255)                                                                    acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa               >  1:1682499/1‑60 (MQ=255)                                                                    acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc    >  1:3451288/1‑71 (MQ=255)                                                                      gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg  <  1:5780/71‑1 (MQ=255)                                                                       acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                >  1:2425160/1‑40 (MQ=255)                                                                       acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACATCTTACGTTAGATTTa                 <  1:2108123/55‑1 (MQ=255)                                                                       |                                                                      CACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCG  >  minE/454610‑454749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑