Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	454,684	G→T	7.5%	P134Q (CCG→CAG) ‡	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	454,684	0	G	T	7.5%	300.3 / 6.5	93	P134Q (CCG→CAG) ‡	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (47/39);  new base T (5/2);  total (52/41)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGA  >  minE/454616‑454753                                                                     |                                                                      cGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGa                                                                     <  1:2740831/71‑1 (MQ=255)  gATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGAt                                                                    >  1:828148/1‑71 (MQ=255)    tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGAt                                                                    <  1:1862105/69‑1 (MQ=255)    tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCg                                                                  >  1:3902234/1‑71 (MQ=255)    tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCg                                                                  >  1:1665292/1‑71 (MQ=255)      ggTGCGATAATAGACCCCACTTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                                <  1:549847/71‑1 (MQ=255)      ggTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                                 <  1:900590/70‑1 (MQ=255)      ggTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                                 <  1:1987309/70‑1 (MQ=255)       gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCgg                                                                      >  1:1140057/1‑64 (MQ=255)       gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                                <  1:524166/70‑1 (MQ=255)       gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                                <  1:3295401/70‑1 (MQ=255)          cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                            >  1:1569829/1‑71 (MQ=255)          cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                            >  1:1172243/1‑71 (MQ=255)            ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGAcgcg                                                                       <  1:1952022/58‑1 (MQ=255)             taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                         >  1:2161032/1‑71 (MQ=255)              aataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                                 >  1:1899291/1‑62 (MQ=255)                  gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                               >  1:2036705/1‑60 (MQ=255)                  gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                         <  1:184754/66‑1 (MQ=255)                     cccACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                       <  1:859548/65‑1 (MQ=255)                     cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc                                                 >  1:3847756/1‑71 (MQ=255)                       cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGc                                               >  1:608391/1‑71 (MQ=255)                        aCGTAACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTg                                                              >  1:831538/1‑55 (MQ=255)                         cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                             <  1:1312282/71‑1 (MQ=255)                          gTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGa                                            <  1:2009645/71‑1 (MQ=255)                           ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                           <  1:276893/71‑1 (MQ=255)                             aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                           >  1:1799032/1‑69 (MQ=255)                             aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                           >  1:373191/1‑69 (MQ=255)                              cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                           >  1:2578201/1‑68 (MQ=255)                              cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                         <  1:2559078/70‑1 (MQ=255)                               ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                       >  1:2965736/1‑71 (MQ=255)                               ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                       >  1:2391635/1‑71 (MQ=255)                                cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                       >  1:3608211/1‑70 (MQ=255)                                 gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                              <  1:2241698/62‑1 (MQ=255)                                 gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                       >  1:3541892/1‑69 (MQ=255)                                 cccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCTGTTGGCTCATACAGCTCGcc                                              <  1:3619297/61‑1 (MQ=255)                                  cagcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                    >  1:2326625/3‑71 (MQ=255)                                   cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGc                                   >  1:2477066/1‑71 (MQ=255)                                    gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                  >  1:1069030/1‑71 (MQ=255)                                    gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                  >  1:405055/1‑71 (MQ=255)                                    gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                  <  1:829819/71‑1 (MQ=255)                                    gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                  <  1:3281296/71‑1 (MQ=255)                                    gcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                  >  1:3766968/1‑71 (MQ=255)                                     cATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCtt                                 <  1:3802070/71‑1 (MQ=255)                                      aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                <  1:1096568/71‑3 (MQ=255)                                        aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                   <  1:2158513/50‑1 (MQ=255)                                        aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACg                              >  1:3177783/1‑71 (MQ=255)                                        aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTAACg                              >  1:165886/1‑71 (MQ=255)                                         cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                             <  1:1233663/71‑1 (MQ=255)                                         cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                             <  1:501747/71‑1 (MQ=255)                                         cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                             >  1:3877817/1‑71 (MQ=255)                                          aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                      <  1:1081706/45‑1 (MQ=255)                                            cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                             >  1:2540867/1‑68 (MQ=255)                                            cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                             >  1:1202482/1‑68 (MQ=255)                                             aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                             >  1:2064997/1‑67 (MQ=255)                                              gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                         <  1:3110265/38‑1 (MQ=255)                                              gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                  <  1:3103711/61‑1 (MQ=255)                                               cGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                             >  1:2622821/1‑49 (MQ=255)                                               cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                       >  1:3321801/1‑71 (MQ=255)                                               cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                       >  1:402654/1‑71 (MQ=255)                                                gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                           <  1:3643367/50‑1 (MQ=255)                                                gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                       >  1:1724687/1‑70 (MQ=255)                                                 agaAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                         >  1:78781/1‑35 (MQ=255)                                                 agaAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                      >  1:2698670/1‑38 (MQ=255)                                                 agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                   <  1:3723749/41‑1 (MQ=255)                                                 agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                        >  1:1792505/1‑68 (MQ=255)                                                 aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttt                     <  1:407424/69‑2 (MQ=255)                                                  gaAACCGCCATCACGACGCGGATCGGTTGGCTCATTCAGCTCGCCGAGCAGACAGCTTACGTTAGAt                        >  1:2123033/1‑67 (MQ=255)                                                  gaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                    <  1:559351/71‑1 (MQ=255)                                                     aCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGtt                            >  1:2765125/1‑60 (MQ=255)                                                     aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCg                                                >  1:1895018/1‑40 (MQ=255)                                                      ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                <  1:683214/71‑1 (MQ=255)                                                      ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                <  1:1102261/71‑1 (MQ=255)                                                       cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                  <  1:2614407/52‑1 (MQ=255)                                                       cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAgg               >  1:2730951/1‑71 (MQ=255)                                                       cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                <  1:3693585/70‑1 (MQ=255)                                                       cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCAGAGCAGACAGCTTACGTTAGATTTAAACAgg               >  1:2724593/1‑71 (MQ=255)                                                        gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                        >  1:3615566/1‑61 (MQ=255)                                                        gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                        >  1:2097698/1‑61 (MQ=255)                                                         ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                  <  1:484344/50‑1 (MQ=255)                                                         ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                >  1:783409/1‑52 (MQ=255)                                                         ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                >  1:968076/1‑52 (MQ=255)                                                         ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                >  1:50847/1‑52 (MQ=255)                                                          cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                         <  1:3866321/42‑1 (MQ=255)                                                          cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa            <  1:1801350/71‑1 (MQ=255)                                                           aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGc                                          >  1:3345456/1‑40 (MQ=255)                                                           aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt           <  1:813998/71‑1 (MQ=255)                                                            tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa            >  1:246003/1‑69 (MQ=255)                                                            tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa          <  1:3780608/71‑1 (MQ=255)                                                            tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa          <  1:2751043/71‑1 (MQ=255)                                                             cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACa                 >  1:968035/1‑63 (MQ=255)                                                              acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                   >  1:1682499/1‑60 (MQ=255)                                                              acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc        >  1:3451288/1‑71 (MQ=255)                                                                gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg      <  1:5780/71‑1 (MQ=255)                                                                 acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                    >  1:2425160/1‑40 (MQ=255)                                                                 acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACATCTTACGTTAGATTTa                     <  1:2108123/55‑1 (MQ=255)                                                                  cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                    >  1:870356/1‑39 (MQ=255)                                                                  cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                    >  1:3792344/1‑39 (MQ=255)                                                                  cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg      >  1:1228929/1‑69 (MQ=255)                                                                    cgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGa  <  1:2779507/71‑1 (MQ=255)                                                                     |                                                                      CGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGA  >  minE/454616‑454753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑