Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	454,690	G→T	7.5%	T132N (ACC→AAC)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	454,690	0	G	T	7.5%	300.2 / 6.5	92	T132N (ACC→AAC)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (47/38);  new base T (5/2);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

GGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAT  >  minE/454621‑454757                                                                      |                                                                    ggTGCGATAATAGACCCCACTTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                                    <  1:549847/71‑1 (MQ=255) ggTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                                     <  1:1987309/70‑1 (MQ=255) ggTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                                     <  1:900590/70‑1 (MQ=255)  gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                                    <  1:524166/70‑1 (MQ=255)  gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                                    <  1:3295401/70‑1 (MQ=255)     cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                                >  1:1172243/1‑71 (MQ=255)     cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                                >  1:1569829/1‑71 (MQ=255)        taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                             >  1:2161032/1‑71 (MQ=255)         aataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCgg                                                                     >  1:1899291/1‑62 (MQ=255)             gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                                   >  1:2036705/1‑60 (MQ=255)             gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                             <  1:184754/66‑1 (MQ=255)                cccACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                           <  1:859548/65‑1 (MQ=255)                cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc                                                     >  1:3847756/1‑71 (MQ=255)                  cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGc                                                   >  1:608391/1‑71 (MQ=255)                   aCGTAACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTg                                                                  >  1:831538/1‑55 (MQ=255)                    cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                 <  1:1312282/71‑1 (MQ=255)                     gTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGa                                                <  1:2009645/71‑1 (MQ=255)                      ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                               <  1:276893/71‑1 (MQ=255)                        aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                               >  1:1799032/1‑69 (MQ=255)                        aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                               >  1:373191/1‑69 (MQ=255)                         cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                               >  1:2578201/1‑68 (MQ=255)                         cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                             <  1:2559078/70‑1 (MQ=255)                          ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                           >  1:2965736/1‑71 (MQ=255)                          ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                           >  1:2391635/1‑71 (MQ=255)                           cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                           >  1:3608211/1‑70 (MQ=255)                            gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                                  <  1:2241698/62‑1 (MQ=255)                            gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                           >  1:3541892/1‑69 (MQ=255)                            cccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCTGTTGGCTCATACAGCTCGcc                                                  <  1:3619297/61‑1 (MQ=255)                             cagcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                        >  1:2326625/3‑71 (MQ=255)                              cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGc                                       >  1:2477066/1‑71 (MQ=255)                               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                      <  1:3281296/71‑1 (MQ=255)                               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                      >  1:405055/1‑71 (MQ=255)                               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                      <  1:829819/71‑1 (MQ=255)                               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                      >  1:1069030/1‑71 (MQ=255)                               gcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                      >  1:3766968/1‑71 (MQ=255)                                cATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCtt                                     <  1:3802070/71‑1 (MQ=255)                                 aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                    <  1:1096568/71‑3 (MQ=255)                                   aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                       <  1:2158513/50‑1 (MQ=255)                                   aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACg                                  >  1:3177783/1‑71 (MQ=255)                                   aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTAACg                                  >  1:165886/1‑71 (MQ=255)                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                 >  1:3877817/1‑71 (MQ=255)                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                 <  1:501747/71‑1 (MQ=255)                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                 <  1:1233663/71‑1 (MQ=255)                                     aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                          <  1:1081706/45‑1 (MQ=255)                                       cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                 >  1:1202482/1‑68 (MQ=255)                                       cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                 >  1:2540867/1‑68 (MQ=255)                                        aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                 >  1:2064997/1‑67 (MQ=255)                                         gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                             <  1:3110265/38‑1 (MQ=255)                                         gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                      <  1:3103711/61‑1 (MQ=255)                                          cGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                 >  1:2622821/1‑49 (MQ=255)                                          cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                           >  1:402654/1‑71 (MQ=255)                                          cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                           >  1:3321801/1‑71 (MQ=255)                                           gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                               <  1:3643367/50‑1 (MQ=255)                                           gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                           >  1:1724687/1‑70 (MQ=255)                                            agaAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                             >  1:78781/1‑35 (MQ=255)                                            agaAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                          >  1:2698670/1‑38 (MQ=255)                                            agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                       <  1:3723749/41‑1 (MQ=255)                                            agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                            >  1:1792505/1‑68 (MQ=255)                                            aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttt                         <  1:407424/69‑2 (MQ=255)                                             gaAACCGCCATCACGACGCGGATCGGTTGGCTCATTCAGCTCGCCGAGCAGACAGCTTACGTTAGAt                            >  1:2123033/1‑67 (MQ=255)                                             gaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                        <  1:559351/71‑1 (MQ=255)                                                aCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGtt                                >  1:2765125/1‑60 (MQ=255)                                                aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCg                                                    >  1:1895018/1‑40 (MQ=255)                                                 ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                    <  1:683214/71‑1 (MQ=255)                                                 ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                    <  1:1102261/71‑1 (MQ=255)                                                  cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                      <  1:2614407/52‑1 (MQ=255)                                                  cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAgg                   >  1:2730951/1‑71 (MQ=255)                                                  cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                    <  1:3693585/70‑1 (MQ=255)                                                  cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCAGAGCAGACAGCTTACGTTAGATTTAAACAgg                   >  1:2724593/1‑71 (MQ=255)                                                   gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                            >  1:3615566/1‑61 (MQ=255)                                                   gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                            >  1:2097698/1‑61 (MQ=255)                                                    ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                      <  1:484344/50‑1 (MQ=255)                                                    ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                    >  1:50847/1‑52 (MQ=255)                                                    ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                    >  1:783409/1‑52 (MQ=255)                                                    ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                    >  1:968076/1‑52 (MQ=255)                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                             <  1:3866321/42‑1 (MQ=255)                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                <  1:1801350/71‑1 (MQ=255)                                                      aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGc                                              >  1:3345456/1‑40 (MQ=255)                                                      aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt               <  1:813998/71‑1 (MQ=255)                                                       tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                >  1:246003/1‑69 (MQ=255)                                                       tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa              <  1:2751043/71‑1 (MQ=255)                                                       tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa              <  1:3780608/71‑1 (MQ=255)                                                        cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACa                     >  1:968035/1‑63 (MQ=255)                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                       >  1:1682499/1‑60 (MQ=255)                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc            >  1:3451288/1‑71 (MQ=255)                                                           gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg          <  1:5780/71‑1 (MQ=255)                                                            acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                        >  1:2425160/1‑40 (MQ=255)                                                            acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACATCTTACGTTAGATTTa                         <  1:2108123/55‑1 (MQ=255)                                                             cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                        >  1:3792344/1‑39 (MQ=255)                                                             cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                        >  1:870356/1‑39 (MQ=255)                                                             cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg          >  1:1228929/1‑69 (MQ=255)                                                               cgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGa      <  1:2779507/71‑1 (MQ=255)                                                                  aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa   <  1:2758569/71‑1 (MQ=255)                                                                   tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa   >  1:709944/1‑70 (MQ=255)                                                                   tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt  <  1:476305/71‑1 (MQ=255)                                                                      gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                <  1:2172007/38‑1 (MQ=255)                                                                      gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                          <  1:645129/44‑1 (MQ=255)                                                                      gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                          <  1:2049359/44‑1 (MQ=255)                                                                      |                                                                    GGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAT  >  minE/454621‑454757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑