| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,211 | T→C | 11.9% | S236S (AGT→AGC) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,211 | 0 | T | C | 11.9% | 176.8 / 12.6 | 67 | S236S (AGT→AGC) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (31/28); new base C (2/6); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGC > minE/189141‑189278 | gTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGt < 1:3892865/71‑1 (MQ=255) tatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGc < 1:1843947/71‑1 (MQ=255) tCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg > 1:1964065/1‑71 (MQ=255) tCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg > 1:2600727/1‑71 (MQ=255) ttACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:3141094/71‑1 (MQ=255) ttACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:1473330/71‑1 (MQ=255) aCCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGAt > 1:690367/1‑71 (MQ=255) ccAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGa < 1:235535/69‑1 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:2743344/71‑1 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:3005263/71‑1 (MQ=255) aCGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc > 1:820214/1‑68 (MQ=255) aCGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc > 1:3720346/1‑68 (MQ=255) ggAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg < 1:1628883/60‑1 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:233731/1‑71 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:6910/1‑71 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:930947/1‑71 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTg < 1:1803808/55‑1 (MQ=255) aCGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCa > 1:1704588/1‑71 (MQ=255) ctgcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaa < 1:248430/61‑1 (MQ=255) cGTCGAACAAAATCGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGa > 1:1360648/1‑58 (MQ=255) tgcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCAc < 1:2711827/43‑1 (MQ=255) tgcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCAc < 1:2661353/43‑1 (MQ=255) gTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa < 1:3052458/63‑1 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGc < 1:3079407/46‑1 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaa < 1:3226810/71‑1 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaa > 1:3658149/2‑62 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa > 1:1595306/1‑71 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaa < 1:958532/70‑1 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaa < 1:1529292/70‑1 (MQ=255) gAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACAAAt < 1:1642960/71‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaaa < 1:3724611/70‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:3053044/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc > 1:632539/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3667322/71‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3354540/71‑1 (MQ=255) aCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCg > 1:1985098/1‑71 (MQ=255) aCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCg > 1:2615817/1‑71 (MQ=255) cAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGc > 1:1673698/1‑71 (MQ=255) aaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTg > 1:3691979/1‑71 (MQ=255) aaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGc < 1:1870698/38‑1 (MQ=255) aaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGAt > 1:780675/1‑45 (MQ=255) aaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGAt > 1:1246270/1‑45 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAATGTCATAAATCGCTgg < 1:845810/70‑1 (MQ=255) tgtgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGgcg > 1:1209976/1‑70 (MQ=255) gtgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCa > 1:146827/1‑71 (MQ=255) ggTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCt < 1:2441252/71‑1 (MQ=255) ggTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCt < 1:1883741/71‑1 (MQ=255) gTATGTACGCCGCGCCATCCGGGAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCt < 1:3763025/70‑1 (MQ=255) gTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa > 1:715104/1‑46 (MQ=255) aTGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt < 1:3402489/45‑1 (MQ=255) aTGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt < 1:1532994/45‑1 (MQ=255) aTGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt > 1:3883740/1‑71 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt > 1:224687/1‑70 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:213731/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:2850883/70‑1 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat > 1:3370770/1‑71 (MQ=255) cgccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatga > 1:3658826/1‑71 (MQ=255) cgccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatg < 1:2470767/70‑1 (MQ=255) ccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATa < 1:771326/45‑1 (MQ=255) ccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3501113/49‑1 (MQ=255) cgcgCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatgatg > 1:2218557/1‑70 (MQ=255) gcgcCATCCGGCAGTACGCTGTTGATCGCCCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGaa > 1:3147988/1‑71 (MQ=255) cgcCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAAc < 1:1653186/71‑1 (MQ=255) gcCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAAc > 1:581932/1‑70 (MQ=255) gcCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACg < 1:1890757/71‑1 (MQ=255) cATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGAt > 1:3187404/1‑71 (MQ=255) aTCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATg > 1:1249521/1‑71 (MQ=255) gTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGc > 1:3388078/1‑69 (MQ=255) tACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGt < 1:2320742/63‑1 (MQ=255) | GTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGC > minE/189141‑189278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |