BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	454,698	A→G	7.3%	Y129Y (TAT→TAC)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	454,698	0	A	G	7.3%	270.8 / 9.6	96	Y129Y (TAT→TAC)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (48/41); new base G (5/2); total (53/43)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 101 total aligned reads displayed.

TAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGG  >  minE/454628‑454767

taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                       >  1:2161032/1‑71 (MQ=255)

     gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                       <  1:184754/66‑1 (MQ=255)

        cccACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                                     <  1:859548/65‑1 (MQ=255)

        cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc                                                               >  1:3847756/1‑71 (MQ=255)

          cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGc                                                             >  1:608391/1‑71 (MQ=255)

            cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                           <  1:1312282/71‑1 (MQ=255)

             gTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGa                                                          <  1:2009645/71‑1 (MQ=255)

              ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                         <  1:276893/71‑1 (MQ=255)

                aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                         >  1:373191/1‑69 (MQ=255)

                aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                         >  1:1799032/1‑69 (MQ=255)

                 cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                         >  1:2578201/1‑68 (MQ=255)

                 cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                       <  1:2559078/70‑1 (MQ=255)

                  ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                     >  1:2965736/1‑71 (MQ=255)

                  ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                     >  1:2391635/1‑71 (MQ=255)

                   cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                     >  1:3608211/1‑70 (MQ=255)

                    gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                                            <  1:2241698/62‑1 (MQ=255)

                    gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                     >  1:3541892/1‑69 (MQ=255)

                    cccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCTGTTGGCTCATACAGCTCGcc                                                            <  1:3619297/61‑1 (MQ=255)

                     cagcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                                  >  1:2326625/3‑71 (MQ=255)

                      cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGc                                                 >  1:2477066/1‑71 (MQ=255)

                       gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                <  1:3281296/71‑1 (MQ=255)

                       gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                >  1:405055/1‑71 (MQ=255)

                       gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                <  1:829819/71‑1 (MQ=255)

                       gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                >  1:1069030/1‑71 (MQ=255)

                       gcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                                >  1:3766968/1‑71 (MQ=255)

                        cATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCtt                                               <  1:3802070/71‑1 (MQ=255)

                         aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                              <  1:1096568/71‑3 (MQ=255)

                           aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                                 <  1:2158513/50‑1 (MQ=255)

                           aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACg                                            >  1:3177783/1‑71 (MQ=255)

                           aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTAACg                                            >  1:165886/1‑71 (MQ=255)

                            cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                           >  1:3877817/1‑71 (MQ=255)

                            cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                           <  1:501747/71‑1 (MQ=255)

                            cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                           <  1:1233663/71‑1 (MQ=255)

                             aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                                    <  1:1081706/45‑1 (MQ=255)

                               cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                           >  1:2540867/1‑68 (MQ=255)

                               cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                           >  1:1202482/1‑68 (MQ=255)

                                aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                           >  1:2064997/1‑67 (MQ=255)

                                 gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                       <  1:3110265/38‑1 (MQ=255)

                                 gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                <  1:3103711/61‑1 (MQ=255)

                                  cGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                           >  1:2622821/1‑49 (MQ=255)

                                  cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                                     >  1:3321801/1‑71 (MQ=255)

                                  cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                                     >  1:402654/1‑71 (MQ=255)

                                   gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                         <  1:3643367/50‑1 (MQ=255)

                                   gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                     >  1:1724687/1‑70 (MQ=255)

                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                       >  1:78781/1‑35 (MQ=255)

                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                                    >  1:2698670/1‑38 (MQ=255)

                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                                 <  1:3723749/41‑1 (MQ=255)

                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                      >  1:1792505/1‑68 (MQ=255)

                                    aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttt                                   <  1:407424/69‑2 (MQ=255)

                                     gaAACCGCCATCACGACGCGGATCGGTTGGCTCATTCAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                      >  1:2123033/1‑67 (MQ=255)

                                     gaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                  <  1:559351/71‑1 (MQ=255)

                                        aCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGtt                                          >  1:2765125/1‑60 (MQ=255)

                                        aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCg                                                              >  1:1895018/1‑40 (MQ=255)

                                         ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                              <  1:1102261/71‑1 (MQ=255)

                                         ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                              <  1:683214/71‑1 (MQ=255)

                                          cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                <  1:2614407/52‑1 (MQ=255)

                                          cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAgg                             >  1:2730951/1‑71 (MQ=255)

                                          cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                              <  1:3693585/70‑1 (MQ=255)

                                          cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCAGAGCAGACAGCTTACGTTAGATTTAAACAgg                             >  1:2724593/1‑71 (MQ=255)

                                           gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                      >  1:3615566/1‑61 (MQ=255)

                                           gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                      >  1:2097698/1‑61 (MQ=255)

                                            ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                <  1:484344/50‑1 (MQ=255)

                                            ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                              >  1:783409/1‑52 (MQ=255)

                                            ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                              >  1:50847/1‑52 (MQ=255)

                                             cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                       <  1:3866321/42‑1 (MQ=255)

                                             cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                          <  1:1801350/71‑1 (MQ=255)

                                              aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGc                                                        >  1:3345456/1‑40 (MQ=255)

                                              aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                         <  1:813998/71‑1 (MQ=255)

                                               tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                          >  1:246003/1‑69 (MQ=255)

                                               tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa                        <  1:3780608/71‑1 (MQ=255)

                                               tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa                        <  1:2751043/71‑1 (MQ=255)

                                                cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACa                               >  1:968035/1‑63 (MQ=255)

                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                 >  1:1682499/1‑60 (MQ=255)

                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc                      >  1:3451288/1‑71 (MQ=255)

                                                   gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                    <  1:5780/71‑1 (MQ=255)

                                                    acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                  >  1:2425160/1‑40 (MQ=255)

                                                    acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACATCTTACGTTAGATTTa                                   <  1:2108123/55‑1 (MQ=255)

                                                     cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                  >  1:3792344/1‑39 (MQ=255)

                                                     cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                  >  1:870356/1‑39 (MQ=255)

                                                     cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                    >  1:1228929/1‑69 (MQ=255)

                                                       cgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGa                <  1:2779507/71‑1 (MQ=255)

                                                          aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa             <  1:2758569/71‑1 (MQ=255)

                                                           tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa             >  1:709944/1‑70 (MQ=255)

                                                           tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt            <  1:476305/71‑1 (MQ=255)

                                                              gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                          <  1:2172007/38‑1 (MQ=255)

                                                              gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                    <  1:2049359/44‑1 (MQ=255)

                                                              gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                    <  1:645129/44‑1 (MQ=255)

                                                                  gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                 >  1:2483421/1‑43 (MQ=255)

                                                                  gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc     <  1:816773/71‑1 (MQ=255)

                                                                  gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc     <  1:3879563/71‑1 (MQ=255)

                                                                  gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc     <  1:386479/71‑1 (MQ=255)

                                                                  gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc      <  1:1096232/70‑1 (MQ=255)

                                                                  ccTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc      <  1:3247900/69‑1 (MQ=255)

                                                                   cTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc      >  1:281619/1‑69 (MQ=255)

                                                                     cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg  >  1:2851010/1‑71 (MQ=255)

                                                                     cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg  >  1:311638/1‑71 (MQ=255)

                                                                     cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg  >  1:1456961/1‑71 (MQ=255)

                                                                     cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg  >  1:316507/1‑71 (MQ=255)

                                                                     cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg  >  1:3742428/1‑71 (MQ=255)

                                                                      aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTATACAGGCCATAGCCGCAGACGATAATCGCCAgg  <  1:3081533/70‑1 (MQ=255)

TAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGG  >  minE/454628‑454767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`