BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	454,701	C→G	7.3%	L128L (CTG→CTC)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	454,701	0	C	G	7.3%	282.9 / 8.0	95	L128L (CTG→CTC)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (47/41); new base G (5/2); total (52/43)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 106 total aligned reads displayed.

CCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACA  >  minE/454636‑454771

cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc                                                                   >  1:3847756/1‑71 (MQ=255)

  cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGc                                                                 >  1:608391/1‑71 (MQ=255)

    cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                               <  1:1312282/71‑1 (MQ=255)

     gTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGa                                                              <  1:2009645/71‑1 (MQ=255)

      ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                             <  1:276893/71‑1 (MQ=255)

        aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                             >  1:1799032/1‑69 (MQ=255)

        aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                             >  1:373191/1‑69 (MQ=255)

         cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                             >  1:2578201/1‑68 (MQ=255)

          ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                         >  1:2391635/1‑71 (MQ=255)

           cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                         >  1:3608211/1‑70 (MQ=255)

            gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                                                <  1:2241698/62‑1 (MQ=255)

            gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                         >  1:3541892/1‑69 (MQ=255)

            cccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCTGTTGGCTCATACAGCTCGcc                                                                <  1:3619297/61‑1 (MQ=255)

             cagcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                                      >  1:2326625/3‑71 (MQ=255)

              cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGc                                                     >  1:2477066/1‑71 (MQ=255)

               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                    <  1:3281296/71‑1 (MQ=255)

               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                    <  1:829819/71‑1 (MQ=255)

               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                    >  1:1069030/1‑71 (MQ=255)

               gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                    >  1:405055/1‑71 (MQ=255)

               gcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                                    >  1:3766968/1‑71 (MQ=255)

                cATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCtt                                                   <  1:3802070/71‑1 (MQ=255)

                 aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                                  <  1:1096568/71‑3 (MQ=255)

                   aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                                     <  1:2158513/50‑1 (MQ=255)

                   aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACg                                                >  1:3177783/1‑71 (MQ=255)

                   aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTAACg                                                >  1:165886/1‑71 (MQ=255)

                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                               <  1:501747/71‑1 (MQ=255)

                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                               >  1:3877817/1‑71 (MQ=255)

                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                               <  1:1233663/71‑1 (MQ=255)

                     aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                                        <  1:1081706/45‑1 (MQ=255)

                       cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                               >  1:1202482/1‑68 (MQ=255)

                       cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                               >  1:2540867/1‑68 (MQ=255)

                        aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                               >  1:2064997/1‑67 (MQ=255)

                         gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                    <  1:3103711/61‑1 (MQ=255)

                          cGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                               >  1:2622821/1‑49 (MQ=255)

                          cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                                         >  1:402654/1‑71 (MQ=255)

                          cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                                         >  1:3321801/1‑71 (MQ=255)

                           gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                             <  1:3643367/50‑1 (MQ=255)

                           gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                         >  1:1724687/1‑70 (MQ=255)

                            agaAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                                        >  1:2698670/1‑38 (MQ=255)

                            agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                                     <  1:3723749/41‑1 (MQ=255)

                            agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                          >  1:1792505/1‑68 (MQ=255)

                            aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttt                                       <  1:407424/69‑2 (MQ=255)

                             gaAACCGCCATCACGACGCGGATCGGTTGGCTCATTCAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                          >  1:2123033/1‑67 (MQ=255)

                             gaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                      <  1:559351/71‑1 (MQ=255)

                                aCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGtt                                              >  1:2765125/1‑60 (MQ=255)

                                aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCg                                                                  >  1:1895018/1‑40 (MQ=255)

                                 ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                                  <  1:683214/71‑1 (MQ=255)

                                 ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                                  <  1:1102261/71‑1 (MQ=255)

                                  cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                    <  1:2614407/52‑1 (MQ=255)

                                  cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAgg                                 >  1:2730951/1‑71 (MQ=255)

                                  cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCAGAGCAGACAGCTTACGTTAGATTTAAACAgg                                 >  1:2724593/1‑71 (MQ=255)

                                   gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                          >  1:2097698/1‑61 (MQ=255)

                                   gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                          >  1:3615566/1‑61 (MQ=255)

                                    ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                    <  1:484344/50‑1 (MQ=255)

                                    ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                                  >  1:783409/1‑52 (MQ=255)

                                    ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                                  >  1:50847/1‑52 (MQ=255)

                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                           <  1:3866321/42‑1 (MQ=255)

                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                              <  1:1801350/71‑1 (MQ=255)

                                      aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGc                                                            >  1:3345456/1‑40 (MQ=255)

                                      aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                             <  1:813998/71‑1 (MQ=255)

                                       tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                              >  1:246003/1‑69 (MQ=255)

                                       tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa                            <  1:3780608/71‑1 (MQ=255)

                                       tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa                            <  1:2751043/71‑1 (MQ=255)

                                        cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACa                                   >  1:968035/1‑63 (MQ=255)

                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                     >  1:1682499/1‑60 (MQ=255)

                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc                          >  1:3451288/1‑71 (MQ=255)

                                           gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                        <  1:5780/71‑1 (MQ=255)

                                            acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                      >  1:2425160/1‑40 (MQ=255)

                                            acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACATCTTACGTTAGATTTa                                       <  1:2108123/55‑1 (MQ=255)

                                             cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                      >  1:870356/1‑39 (MQ=255)

                                             cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                      >  1:3792344/1‑39 (MQ=255)

                                             cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                        >  1:1228929/1‑69 (MQ=255)

                                               cgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGa                    <  1:2779507/71‑1 (MQ=255)

                                                  aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                 <  1:2758569/71‑1 (MQ=255)

                                                   tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                 >  1:709944/1‑70 (MQ=255)

                                                      gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                              <  1:2172007/38‑1 (MQ=255)

                                                      gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                        <  1:2049359/44‑1 (MQ=255)

                                                      gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                        <  1:645129/44‑1 (MQ=255)

                                                          gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                     >  1:2483421/1‑43 (MQ=255)

                                                          gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc         <  1:386479/71‑1 (MQ=255)

                                                          gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc         <  1:3879563/71‑1 (MQ=255)

                                                          gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc         <  1:816773/71‑1 (MQ=255)

                                                          gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc          <  1:1096232/70‑1 (MQ=255)

                                                          ccTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc          <  1:3247900/69‑1 (MQ=255)

                                                           cTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc          >  1:281619/1‑69 (MQ=255)

                                                             cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg      >  1:2851010/1‑71 (MQ=255)

                                                             cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg      >  1:316507/1‑71 (MQ=255)

                                                             cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg      >  1:1456961/1‑71 (MQ=255)

                                                             cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg      >  1:311638/1‑71 (MQ=255)

                                                             cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg      >  1:3742428/1‑71 (MQ=255)

                                                              aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTATACAGGCCATAGCCGCAGACGATAATCGCCAgg      <  1:3081533/70‑1 (MQ=255)

                                                                aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg       <  1:2941641/67‑1 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                     >  1:599382/1‑36 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa    >  1:3560681/1‑69 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa    >  1:355510/1‑69 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa    >  1:1946835/1‑69 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa    >  1:2952482/1‑69 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGAc   >  1:2563548/1‑70 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACa  <  1:3939566/71‑1 (MQ=255)

                                                                 cAGCTCGCCGAGCAGACAGCTAACGTTAGATTTAAACa                                   >  1:3810630/1‑38 (MQ=255)

CCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACA  >  minE/454636‑454771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`