BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	454,710	G→A	6.7%	L125L (CTC→CTT)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	454,710	0	G	A	6.7%	322.4 / 7.9	104	L125L (CTC→CTT)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand): ref base G (57/40); new base A (5/2); total (62/42)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGG  >  minE/454640‑454779

cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                                       <  1:1312282/71‑1 (MQ=255)

 gTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGa                                                                      <  1:2009645/71‑1 (MQ=255)

  ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     <  1:276893/71‑1 (MQ=255)

    aCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     >  1:1799032/1‑69 (MQ=255)

     cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     >  1:2578201/1‑68 (MQ=255)

     cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                                   <  1:2559078/70‑1 (MQ=255)

      ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                                 >  1:2391635/1‑71 (MQ=255)

       cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                                 >  1:3608211/1‑70 (MQ=255)

        gccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                                 >  1:3541892/1‑69 (MQ=255)

         cagcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                                              >  1:2326625/3‑71 (MQ=255)

          cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGc                                                             >  1:2477066/1‑71 (MQ=255)

           gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            >  1:1069030/1‑71 (MQ=255)

           gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            >  1:405055/1‑71 (MQ=255)

           gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:3281296/71‑1 (MQ=255)

           gcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:829819/71‑1 (MQ=255)

           gcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                                            >  1:3766968/1‑71 (MQ=255)

            cATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCtt                                                           <  1:3802070/71‑1 (MQ=255)

             aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                                          <  1:1096568/71‑3 (MQ=255)

               aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACg                                                        >  1:3177783/1‑71 (MQ=255)

               aCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTAACg                                                        >  1:165886/1‑71 (MQ=255)

                cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       <  1:501747/71‑1 (MQ=255)

                cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       >  1:3877817/1‑71 (MQ=255)

                cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       <  1:1233663/71‑1 (MQ=255)

                   cAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       >  1:1202482/1‑68 (MQ=255)

                    aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       >  1:2064997/1‑67 (MQ=255)

                     gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:3103711/61‑1 (MQ=255)

                      cGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                                       >  1:2622821/1‑49 (MQ=255)

                      cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                                                 >  1:3321801/1‑71 (MQ=255)

                      cGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtt                                                 >  1:402654/1‑71 (MQ=255)

                       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     <  1:3643367/50‑1 (MQ=255)

                       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                 >  1:1724687/1‑70 (MQ=255)

                        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                  >  1:1792505/1‑68 (MQ=255)

                        aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttt                                               <  1:407424/69‑2 (MQ=255)

                         gaAACCGCCATCACGACGCGGATCGGTTGGCTCATTCAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                  >  1:2123033/1‑67 (MQ=255)

                         gaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                              <  1:559351/71‑1 (MQ=255)

                            aCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGtt                                                      >  1:2765125/1‑60 (MQ=255)

                             ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                                          <  1:1102261/71‑1 (MQ=255)

                             ccGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                                          <  1:683214/71‑1 (MQ=255)

                              cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:2614407/52‑1 (MQ=255)

                              cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAgg                                         >  1:2730951/1‑71 (MQ=255)

                              cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                                          <  1:3693585/70‑1 (MQ=255)

                              cGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCAGAGCAGACAGCTTACGTTAGATTTAAACAgg                                         >  1:2724593/1‑71 (MQ=255)

                               gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                  >  1:2097698/1‑61 (MQ=255)

                               gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                  >  1:3615566/1‑61 (MQ=255)

                                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:484344/50‑1 (MQ=255)

                                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                                          >  1:783409/1‑52 (MQ=255)

                                 cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                                   <  1:3866321/42‑1 (MQ=255)

                                 cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                                      <  1:1801350/71‑1 (MQ=255)

                                  aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGc                                                                    >  1:3345456/1‑40 (MQ=255)

                                  aTCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                     <  1:813998/71‑1 (MQ=255)

                                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCa                                      >  1:246003/1‑69 (MQ=255)

                                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa                                    <  1:3780608/71‑1 (MQ=255)

                                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATa                                    <  1:2751043/71‑1 (MQ=255)

                                    cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACa                                           >  1:968035/1‑63 (MQ=255)

                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:1682499/1‑60 (MQ=255)

                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc                                  >  1:3451288/1‑71 (MQ=255)

                                       gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                <  1:5780/71‑1 (MQ=255)

                                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                              >  1:2425160/1‑40 (MQ=255)

                                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACATCTTACGTTAGATTTa                                               <  1:2108123/55‑1 (MQ=255)

                                         cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                              >  1:3792344/1‑39 (MQ=255)

                                         cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                              >  1:870356/1‑39 (MQ=255)

                                         cgcgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                >  1:1228929/1‑69 (MQ=255)

                                           cgGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGa                            <  1:2779507/71‑1 (MQ=255)

                                              aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                         <  1:2758569/71‑1 (MQ=255)

                                               tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                         >  1:709944/1‑70 (MQ=255)

                                               tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt                        <  1:476305/71‑1 (MQ=255)

                                                  gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                      <  1:2172007/38‑1 (MQ=255)

                                                  gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                                <  1:2049359/44‑1 (MQ=255)

                                                  gTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                                <  1:645129/44‑1 (MQ=255)

                                                      gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:2483421/1‑43 (MQ=255)

                                                      gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                 <  1:3879563/71‑1 (MQ=255)

                                                      gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                 <  1:816773/71‑1 (MQ=255)

                                                      gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                 <  1:386479/71‑1 (MQ=255)

                                                      gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                  <  1:1096232/70‑1 (MQ=255)

                                                      ccTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                  <  1:3247900/69‑1 (MQ=255)

                                                       cTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                  >  1:281619/1‑69 (MQ=255)

                                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg              >  1:3742428/1‑71 (MQ=255)

                                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg              >  1:1456961/1‑71 (MQ=255)

                                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg              >  1:2851010/1‑71 (MQ=255)

                                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg              >  1:311638/1‑71 (MQ=255)

                                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg              >  1:316507/1‑71 (MQ=255)

                                                          aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTATACAGGCCATAGCCGCAGACGATAATCGCCAgg              <  1:3081533/70‑1 (MQ=255)

                                                            aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg               <  1:2941641/67‑1 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:599382/1‑36 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:1924399/1‑36 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            >  1:355510/1‑69 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            >  1:2952482/1‑69 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            >  1:3560681/1‑69 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            >  1:1946835/1‑69 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGAc           >  1:2563548/1‑70 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACa          <  1:3939566/71‑1 (MQ=255)

                                                             cAGCTCGCCGAGCAGACAGCTAACGTTAGATTTAAACa                                           >  1:3810630/1‑38 (MQ=255)

                                                               gCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa        <  1:77958/71‑1 (MQ=255)

                                                                cTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg                                          >  1:1652159/1‑36 (MQ=255)

                                                                cTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGa                            >  1:2389554/1‑50 (MQ=255)

                                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa      >  1:1249316/1‑69 (MQ=255)

                                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGAAAGATa      >  1:902123/1‑69 (MQ=255)

                                                                    ccGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATagag   >  1:741722/1‑71 (MQ=255)

                                                                    ccGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGAAGACGATAATCGCCAGGGACAGATagag   >  1:244683/1‑71 (MQ=255)

                                                                     cGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAgg  <  1:929960/71‑1 (MQ=255)

CGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGG  >  minE/454640‑454779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`