Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	455,040	A→C	30.5%	V15V (GTT→GTG)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	455,040	0	A	C	30.5%	73.0 / 74.2	82	V15V (GTT→GTG)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (21/36);  new base C (9/16);  total (30/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGT  >  minE/454972‑455110                                                                     |                                                                       ttGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCaa                                                                       >  1:3571166/1‑70 (MQ=255)  tGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCaa                                                                       >  1:2071687/1‑69 (MQ=255)   ggTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACg                                                                     <  1:3413146/70‑1 (MQ=255)   ggTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACg                                                                     <  1:1588269/70‑1 (MQ=255)     tGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGata                                                                  <  1:479848/71‑3 (MQ=255)      gagaTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa                                                                  >  1:2183757/1‑70 (MQ=255)       agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGAtata                                                                <  1:1031011/71‑1 (MQ=255)         aTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGt                                                              <  1:2289002/71‑1 (MQ=255)          tAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTa                                                             >  1:1461850/1‑71 (MQ=255)          tAGAGAATCAGCAGGACACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa                                                             <  1:220749/71‑1 (MQ=255)            gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTata                                                           <  1:2013045/71‑3 (MQ=255)            gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                           <  1:1765787/71‑1 (MQ=255)            gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                           <  1:3595261/71‑1 (MQ=255)            gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                           <  1:1366534/71‑1 (MQ=255)             agaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGAtata                                                                <  1:3048127/65‑1 (MQ=255)              gaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTatatc                                                         <  1:3408650/71‑2 (MQ=255)              gaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTatatc                                                         >  1:665482/1‑70 (MQ=255)              gaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAAt                                                          <  1:1390922/70‑1 (MQ=255)                   agcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgcg                                                    <  1:954568/71‑1 (MQ=255)                      agGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                 >  1:574366/1‑71 (MQ=255)                       gggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGcgcgcg                                                  >  1:1412661/1‑69 (MQ=255)                       gggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGcgcgcg                                                  >  1:3215920/1‑69 (MQ=255)                       aggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGc                                                <  1:742297/70‑1 (MQ=255)                       aggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGc                                                <  1:2253888/70‑1 (MQ=255)                        ggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCt                                               <  1:1765940/71‑1 (MQ=255)                        ggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCt                                               <  1:1058675/71‑1 (MQ=255)                         gCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACg                                                                     <  1:3558903/48‑1 (MQ=255)                         gCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtg                                              <  1:523346/71‑1 (MQ=255)                         gCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCt                                               >  1:925881/1‑70 (MQ=255)                          cACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                             <  1:3820351/71‑1 (MQ=255)                           aCGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGAc                                                                   >  1:2373244/1‑48 (MQ=255)                           aCGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCtgt                                             >  1:2978068/1‑70 (MQ=255)                            cGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgc                                                       >  1:1591018/1‑59 (MQ=255)                            cGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCtgt                                             >  1:2146594/1‑69 (MQ=255)                            cGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGa                                           <  1:2396610/71‑1 (MQ=255)                            cGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGa                                           <  1:1320841/71‑1 (MQ=255)                             gCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGAt                                          >  1:1978969/1‑71 (MQ=255)                             gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTa                                                             <  1:1194229/52‑1 (MQ=255)                             gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTa                                                             <  1:2916924/52‑1 (MQ=255)                             gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                          >  1:3258357/1‑70 (MQ=255)                             gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                          >  1:1627916/1‑70 (MQ=255)                             gCATGCAGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                          >  1:513700/1‑70 (MQ=255)                               aTGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGc                                        >  1:954552/1‑71 (MQ=255)                                tGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                          <  1:3130043/68‑2 (MQ=255)                                  ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgtg                                     >  1:3201955/1‑71 (MQ=255)                                  ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                      <  1:3627767/70‑1 (MQ=255)                                  ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                      <  1:844890/70‑1 (MQ=255)                                  ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                      <  1:970811/70‑1 (MQ=255)                                  ccGTAAAAGCTAAAATATTCCCAGATTTGAAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                      <  1:2950957/70‑1 (MQ=255)                                   cGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAAt                                                          <  1:428715/49‑1 (MQ=255)                                    gTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGatat                                                                 <  1:1236708/41‑1 (MQ=255)                                     tAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa                                                                  <  1:3026772/39‑1 (MQ=255)                                     tAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa                                                                  <  1:3207931/39‑1 (MQ=255)                                     tAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGttt                                  <  1:1840787/71‑1 (MQ=255)                                     tAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGagg                                         <  1:2582739/64‑3 (MQ=255)                                       aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGA‑GGGTGttttca                               <  1:3945306/71‑4 (MQ=255)                                       aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGA‑GGGTGttttca                               >  1:975180/1‑68 (MQ=255)                                        aaGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTAtt                               <  1:1835985/71‑1 (MQ=255)                                         aGCTAAAATATTCCCAGATTTGCAGCGCAACGACATa                                                                >  1:1545148/1‑37 (MQ=255)                                         aGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                             <  1:3140237/56‑1 (MQ=255)                                            tAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                             <  1:3642785/53‑1 (MQ=255)                                            tAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTa                                 <  1:2425680/65‑1 (MQ=255)                                             aaaaTATTCCCAGATTTGCAGCGCAACGACATAGTa                                                             <  1:834973/36‑1 (MQ=255)                                             aaaaTATTCCCAGATTTGCAGCGCAACGACATAGTa                                                             <  1:2826580/36‑1 (MQ=255)                                             aaaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                 <  1:3761966/48‑1 (MQ=255)                                             aaaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                 <  1:2976604/48‑1 (MQ=255)                                              aaaTATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                           <  1:3947282/37‑1 (MQ=255)                                              aaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGa                                           >  1:3120921/1‑53 (MQ=255)                                              aaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGa                                           >  1:768312/1‑53 (MQ=255)                                                atatTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTa                                 <  1:2360379/61‑1 (MQ=255)                                                atatTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCAtaataa                       <  1:1649166/71‑1 (MQ=255)                                                 tatTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                             >  1:1749513/1‑48 (MQ=255)                                                  atTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAAtt                     >  1:1736829/1‑71 (MQ=255)                                                   ttCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTc                    >  1:3255694/1‑71 (MQ=255)                                                    tCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTc                    >  1:2045534/1‑70 (MQ=255)                                                      ccAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcac                 <  1:3367121/71‑1 (MQ=255)                                                      ccAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCca                  <  1:2367512/70‑1 (MQ=255)                                                        aGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcaca                >  1:3313784/1‑70 (MQ=255)                                                        aGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACaa               <  1:775624/71‑1 (MQ=255)                                                         gATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGATTATTCATAATAATTCCACAAt              >  1:2068334/1‑71 (MQ=255)                                                              gCAGCGCATCGACATAGTAAATAGCGCTCGGCTGTGATGCGTGTTTa                                 <  1:3225894/47‑1 (MQ=39)                                                               cAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcaca                >  1:2811476/1‑63 (MQ=255)                                                                aGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACgg        >  1:2086334/1‑70 (MQ=255)                                                                   gcAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAAc    >  1:1308397/1‑71 (MQ=255)                                                                     aaCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGt  >  1:197753/1‑71 (MQ=255)                                                                     aaCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGt  >  1:854809/1‑71 (MQ=255)                                                                     aaCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGt  >  1:316874/1‑71 (MQ=255)                                                                     |                                                                       TTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGT  >  minE/454972‑455110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑