Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	455,052	A→T	20.0%	I11I (ATT→ATA)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	455,052	0	A	T	20.0%	153.0 / 34.0	70	I11I (ATT→ATA)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (27/29);  new base T (7/7);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

GAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGT  >  minE/454983‑455121                                                                      |                                                                      gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                      <  1:3595261/71‑1 (MQ=255) gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                      <  1:1366534/71‑1 (MQ=255) gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                      <  1:1765787/71‑1 (MQ=255)   gaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTatatc                                                                    <  1:3408650/71‑2 (MQ=255)   gaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTatatc                                                                    >  1:665482/1‑70 (MQ=255)   gaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAAt                                                                     <  1:1390922/70‑1 (MQ=255)        agcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgcg                                                               <  1:954568/71‑1 (MQ=255)           agGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                            >  1:574366/1‑71 (MQ=255)            gggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGcgcgcg                                                             >  1:1412661/1‑69 (MQ=255)            gggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGcgcgcg                                                             >  1:3215920/1‑69 (MQ=255)            aggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGc                                                           <  1:2253888/70‑1 (MQ=255)            aggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGc                                                           <  1:742297/70‑1 (MQ=255)             ggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCt                                                          <  1:1765940/71‑1 (MQ=255)             ggCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCt                                                          <  1:1058675/71‑1 (MQ=255)              gCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtg                                                         <  1:523346/71‑1 (MQ=255)              gCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCt                                                          >  1:925881/1‑70 (MQ=255)               cACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                        <  1:3820351/71‑1 (MQ=255)                aCGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCtgt                                                        >  1:2978068/1‑70 (MQ=255)                 cGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgc                                                                  >  1:1591018/1‑59 (MQ=255)                 cGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCtgt                                                        >  1:2146594/1‑69 (MQ=255)                 cGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGa                                                      <  1:1320841/71‑1 (MQ=255)                 cGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGa                                                      <  1:2396610/71‑1 (MQ=255)                  gCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGAt                                                     >  1:1978969/1‑71 (MQ=255)                  gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                                     >  1:1627916/1‑70 (MQ=255)                  gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                                     >  1:3258357/1‑70 (MQ=255)                  gCATGCAGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                                     >  1:513700/1‑70 (MQ=255)                    aTGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGc                                                   >  1:954552/1‑71 (MQ=255)                     tGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                                     <  1:3130043/68‑2 (MQ=255)                       ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgtg                                                >  1:3201955/1‑71 (MQ=255)                       ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                                 <  1:3627767/70‑1 (MQ=255)                       ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                                 <  1:844890/70‑1 (MQ=255)                       ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                                 <  1:970811/70‑1 (MQ=255)                       ccGTAAAAGCTAAAATATTCCCAGATTTGAAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgt                                                 <  1:2950957/70‑1 (MQ=255)                        cGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAAt                                                                     <  1:428715/49‑1 (MQ=255)                          tAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGttt                                             <  1:1840787/71‑1 (MQ=255)                          tAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGagg                                                    <  1:2582739/64‑3 (MQ=255)                            aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGA‑GGGTGttttca                                          <  1:3945306/71‑4 (MQ=255)                            aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGA‑GGGTGttttca                                          >  1:975180/1‑68 (MQ=255)                             aaGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTAtt                                          <  1:1835985/71‑1 (MQ=255)                              aGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                        <  1:3140237/56‑1 (MQ=255)                                 tAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                        <  1:3642785/53‑1 (MQ=255)                                 tAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTa                                            <  1:2425680/65‑1 (MQ=255)                                  aaaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                            <  1:3761966/48‑1 (MQ=255)                                  aaaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                            <  1:2976604/48‑1 (MQ=255)                                   aaaTATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                      <  1:3947282/37‑1 (MQ=255)                                   aaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGa                                                      >  1:3120921/1‑53 (MQ=255)                                   aaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGa                                                      >  1:768312/1‑53 (MQ=255)                                     atatTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTa                                            <  1:2360379/61‑1 (MQ=255)                                     atatTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCAtaataa                                  <  1:1649166/71‑1 (MQ=255)                                      tatTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                        >  1:1749513/1‑48 (MQ=255)                                       atTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAAtt                                >  1:1736829/1‑71 (MQ=255)                                        ttCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTc                               >  1:3255694/1‑71 (MQ=255)                                         tCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTc                               >  1:2045534/1‑70 (MQ=255)                                           ccAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcac                            <  1:3367121/71‑1 (MQ=255)                                           ccAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCca                             <  1:2367512/70‑1 (MQ=255)                                             aGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcaca                           >  1:3313784/1‑70 (MQ=255)                                             aGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACaa                          <  1:775624/71‑1 (MQ=255)                                              gATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGATTATTCATAATAATTCCACAAt                         >  1:2068334/1‑71 (MQ=255)                                                   gCAGCGCATCGACATAGTAAATAGCGCTCGGCTGTGATGCGTGTTTa                                            <  1:3225894/47‑1 (MQ=39)                                                    cAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcaca                           >  1:2811476/1‑63 (MQ=255)                                                     aGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACgg                   >  1:2086334/1‑70 (MQ=255)                                                        gcAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAAc               >  1:1308397/1‑71 (MQ=255)                                                          aaCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGt             >  1:197753/1‑71 (MQ=255)                                                          aaCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGt             >  1:316874/1‑71 (MQ=255)                                                          aaCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGt             >  1:854809/1‑71 (MQ=255)                                                            cGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAAtt                                >  1:2443820/1‑50 (MQ=255)                                                            cGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTAATCATAATAATTCCACAATTGACGGCa                 >  1:2142190/1‑65 (MQ=255)                                                             gACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCAc          >  1:149303/1‑71 (MQ=255)                                                                aTAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGt        >  1:1375216/1‑70 (MQ=255)                                                                 tAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTaa      <  1:2576594/71‑1 (MQ=255)                                                                 tAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTaa      <  1:1664833/71‑1 (MQ=255)                                                                    tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATa    <  1:3792915/70‑1 (MQ=255)                                                                    tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATa    <  1:591364/70‑1 (MQ=255)                                                                    tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAg   <  1:855418/71‑1 (MQ=255)                                                                    tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAg   >  1:2831583/1‑71 (MQ=255)                                                                    tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAg   >  1:1353590/1‑71 (MQ=255)                                                                     aaaTAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGt  >  1:1821709/1‑71 (MQ=255)                                                                      aaTAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGa                      >  1:3001603/1‑50 (MQ=255)                                                                      aaTAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGt  >  1:2918522/1‑70 (MQ=255)                                                                      |                                                                      GAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGT  >  minE/454983‑455121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑