| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 481,654 | T→A | 7.7% | L16Q (CTG→CAG) | tolQ → | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 481,654 | 0 | T | A | 7.7% | 144.3 / 4.1 | 52 | L16Q (CTG→CAG) | tolQ | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Reads supporting (aligned to +/- strand): ref base T (26/22); new base A (2/2); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTATTAT > minE/481587‑481714 | tATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTgg > 1:407996/1‑70 (MQ=255)tATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTgg > 1:456836/1‑70 (MQ=255) ttGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTa < 1:1365291/71‑1 (MQ=255) ttGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCAgg < 1:2036339/68‑1 (MQ=255) ttGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCAGAAGGCTAGCCTTCAggaac < 1:1870057/71‑4 (MQ=38) tCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAAc < 1:3599697/71‑1 (MQ=255) gcgGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACtt < 1:1164664/71‑1 (MQ=255) gcgGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACt < 1:1353986/70‑1 (MQ=255) gAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTAt > 1:2243492/1‑70 (MQ=255) tAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGtt > 1:2141630/1‑71 (MQ=255) tAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGt < 1:2637487/70‑1 (MQ=255) aGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttga > 1:2963653/1‑71 (MQ=255) aGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttga > 1:1536707/1‑71 (MQ=255) aGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCAgg > 1:3318943/1‑53 (MQ=255) aGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCAgg > 1:3232713/1‑53 (MQ=255) cAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgatt > 1:275294/1‑71 (MQ=255) aGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattt < 1:477282/71‑1 (MQ=255) gTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgatttt > 1:3906445/1‑71 (MQ=255) tgactgacATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattttg < 1:3584850/71‑1 (MQ=255) actgacATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattttgat > 1:2577582/1‑71 (MQ=255) actgacATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattttga < 1:2379655/70‑1 (MQ=255) tgacATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTaaa < 1:81429/49‑1 (MQ=255) acATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTaa < 1:238127/46‑1 (MQ=255) acATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGt < 1:1656096/71‑1 (MQ=255) aTGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGttt > 1:2418515/1‑71 (MQ=255) aTGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGttt > 1:3476690/1‑71 (MQ=255) aaTATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTTGTTAAACTTATCATGttgattttgat < 1:2699102/62‑1 (MQ=255) aaTATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgatt > 1:1814924/1‑57 (MQ=255) tatCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTAt < 1:2747008/45‑1 (MQ=255) tatCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTAt < 1:3160771/45‑1 (MQ=255) tatCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCaa < 1:588917/71‑1 (MQ=255) tCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATc > 1:2840926/1‑71 (MQ=255) cTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCg > 1:2500728/1‑70 (MQ=255) gATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGttttt > 1:3036915/1‑61 (MQ=255) gATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATc > 1:932068/1‑71 (MQ=255) gATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATc > 1:3575667/1‑71 (MQ=255) gATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATc > 1:2544461/1‑71 (MQ=255) gATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATc > 1:1175781/1‑71 (MQ=255) ttGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATc > 1:1671283/1‑36 (MQ=255) ttGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgatt < 1:3877902/45‑1 (MQ=255) ttGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgatt < 1:1853649/45‑1 (MQ=255) tGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTg < 1:3296298/70‑1 (MQ=255) tCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc > 1:3490098/1‑70 (MQ=255) tCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc > 1:167930/1‑70 (MQ=255) ccTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCa > 1:195472/1‑71 (MQ=255) cTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGttttt < 1:1550926/52‑1 (MQ=255) ggCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATc < 1:3519691/57‑1 (MQ=255) cTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCaa < 1:878704/48‑1 (MQ=255) cTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCat < 1:2609113/64‑1 (MQ=255) tAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATc > 1:3893511/1‑49 (MQ=255) tAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATtattat > 1:662787/1‑69 (MQ=255) gCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCa > 1:3801503/1‑50 (MQ=255) | TATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTATTAT > minE/481587‑481714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |